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New diagnostic SNP molecular markers for the Mytilus species complex

The development of diagnostic markers has been a long-standing interest of population geneticists as it allows clarification of taxonomic uncertainties. Historically, there has been much debate on the taxonomic status of species belonging to the Mytilus species complex (M. edulis, M. galloprovincial...

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Autores principales: Wilson, Joanna, Matejusova, Iveta, McIntosh, Rebecca E., Carboni, Stefano, Bekaert, Michaël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042762/
https://www.ncbi.nlm.nih.gov/pubmed/30001394
http://dx.doi.org/10.1371/journal.pone.0200654
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author Wilson, Joanna
Matejusova, Iveta
McIntosh, Rebecca E.
Carboni, Stefano
Bekaert, Michaël
author_facet Wilson, Joanna
Matejusova, Iveta
McIntosh, Rebecca E.
Carboni, Stefano
Bekaert, Michaël
author_sort Wilson, Joanna
collection PubMed
description The development of diagnostic markers has been a long-standing interest of population geneticists as it allows clarification of taxonomic uncertainties. Historically, there has been much debate on the taxonomic status of species belonging to the Mytilus species complex (M. edulis, M. galloprovincialis and M. trossulus), and whether they are discrete species. We analysed reference pure specimens of M. edulis, M. galloprovincialis and M. trossulus, using Restriction site associated DNA (RAD) sequencing and identified over 6,000 SNP markers separating the three species unambiguously. We developed a panel of diagnostic SNP markers for the genotyping of Mytilus species complex as well as the identification of hybrids and interspecies introgression events in Mytilus species. We validated a panel of twelve diagnostic SNP markers which can be used for species genotyping. Being able to accurately identify species and hybrids within the Mytilus species complex is important for the selective mussel stock management, the exclusion of invasive species, basic physiology and bio-diversity studies.
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spelling pubmed-60427622018-07-26 New diagnostic SNP molecular markers for the Mytilus species complex Wilson, Joanna Matejusova, Iveta McIntosh, Rebecca E. Carboni, Stefano Bekaert, Michaël PLoS One Research Article The development of diagnostic markers has been a long-standing interest of population geneticists as it allows clarification of taxonomic uncertainties. Historically, there has been much debate on the taxonomic status of species belonging to the Mytilus species complex (M. edulis, M. galloprovincialis and M. trossulus), and whether they are discrete species. We analysed reference pure specimens of M. edulis, M. galloprovincialis and M. trossulus, using Restriction site associated DNA (RAD) sequencing and identified over 6,000 SNP markers separating the three species unambiguously. We developed a panel of diagnostic SNP markers for the genotyping of Mytilus species complex as well as the identification of hybrids and interspecies introgression events in Mytilus species. We validated a panel of twelve diagnostic SNP markers which can be used for species genotyping. Being able to accurately identify species and hybrids within the Mytilus species complex is important for the selective mussel stock management, the exclusion of invasive species, basic physiology and bio-diversity studies. Public Library of Science 2018-07-12 /pmc/articles/PMC6042762/ /pubmed/30001394 http://dx.doi.org/10.1371/journal.pone.0200654 Text en © 2018 Wilson et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wilson, Joanna
Matejusova, Iveta
McIntosh, Rebecca E.
Carboni, Stefano
Bekaert, Michaël
New diagnostic SNP molecular markers for the Mytilus species complex
title New diagnostic SNP molecular markers for the Mytilus species complex
title_full New diagnostic SNP molecular markers for the Mytilus species complex
title_fullStr New diagnostic SNP molecular markers for the Mytilus species complex
title_full_unstemmed New diagnostic SNP molecular markers for the Mytilus species complex
title_short New diagnostic SNP molecular markers for the Mytilus species complex
title_sort new diagnostic snp molecular markers for the mytilus species complex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042762/
https://www.ncbi.nlm.nih.gov/pubmed/30001394
http://dx.doi.org/10.1371/journal.pone.0200654
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