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Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers
BACKGROUND: RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every molecule amplifies equally, causing some to be overrepresente...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044086/ https://www.ncbi.nlm.nih.gov/pubmed/30001700 http://dx.doi.org/10.1186/s12864-018-4933-1 |