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In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor
BACKGROUND: Cytokeratin 19 (CK19) is a typical epithelial marker. In this study, we determined whether epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) could enhance CK19 expression in adipose-derived stem cells (ADSCs), thereby inducing the differentiation of ADSCs into epithe...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044214/ https://www.ncbi.nlm.nih.gov/pubmed/29925829 http://dx.doi.org/10.12659/MSM.908647 |
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author | Chen, Shangliang Wang, Mingzhu Chen, Xinglu Chen, Shaolian Liu, Li Zhu, Jianbin Wang, Jinhui Yang, Xiaorong Cai, Xiangsheng |
author_facet | Chen, Shangliang Wang, Mingzhu Chen, Xinglu Chen, Shaolian Liu, Li Zhu, Jianbin Wang, Jinhui Yang, Xiaorong Cai, Xiangsheng |
author_sort | Chen, Shangliang |
collection | PubMed |
description | BACKGROUND: Cytokeratin 19 (CK19) is a typical epithelial marker. In this study, we determined whether epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) could enhance CK19 expression in adipose-derived stem cells (ADSCs), thereby inducing the differentiation of ADSCs into epithelial-like cells. MATERIAL/METHODS: ADSCs were isolated from perinephric fat, and the expression of CD29, CD90, and CD105 was confirmed. Following isolation, ADSCs were cultured in static medium or medium containing EGF or bFGF. RESULTS: Flow cytometry revealed that EGF and bFGF could alter mesenchymal stem cell markers as well as the cell cycle of ADSCs. Western blotting and immunofluorescence revealed that after 14 days, EGF treatment enhanced the expression of CK19 in ADSCs. CONCLUSIONS: Our findings offer important insight for the clinical use of ADSCs in the generation of epithelial-like cells in the future. |
format | Online Article Text |
id | pubmed-6044214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60442142018-07-13 In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor Chen, Shangliang Wang, Mingzhu Chen, Xinglu Chen, Shaolian Liu, Li Zhu, Jianbin Wang, Jinhui Yang, Xiaorong Cai, Xiangsheng Med Sci Monit Lab/In Vitro Research BACKGROUND: Cytokeratin 19 (CK19) is a typical epithelial marker. In this study, we determined whether epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) could enhance CK19 expression in adipose-derived stem cells (ADSCs), thereby inducing the differentiation of ADSCs into epithelial-like cells. MATERIAL/METHODS: ADSCs were isolated from perinephric fat, and the expression of CD29, CD90, and CD105 was confirmed. Following isolation, ADSCs were cultured in static medium or medium containing EGF or bFGF. RESULTS: Flow cytometry revealed that EGF and bFGF could alter mesenchymal stem cell markers as well as the cell cycle of ADSCs. Western blotting and immunofluorescence revealed that after 14 days, EGF treatment enhanced the expression of CK19 in ADSCs. CONCLUSIONS: Our findings offer important insight for the clinical use of ADSCs in the generation of epithelial-like cells in the future. International Scientific Literature, Inc. 2018-06-21 /pmc/articles/PMC6044214/ /pubmed/29925829 http://dx.doi.org/10.12659/MSM.908647 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Lab/In Vitro Research Chen, Shangliang Wang, Mingzhu Chen, Xinglu Chen, Shaolian Liu, Li Zhu, Jianbin Wang, Jinhui Yang, Xiaorong Cai, Xiangsheng In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor |
title | In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor |
title_full | In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor |
title_fullStr | In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor |
title_full_unstemmed | In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor |
title_short | In Vitro Expression of Cytokeratin 19 in Adipose-Derived Stem Cells Is Induced by Epidermal Growth Factor |
title_sort | in vitro expression of cytokeratin 19 in adipose-derived stem cells is induced by epidermal growth factor |
topic | Lab/In Vitro Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044214/ https://www.ncbi.nlm.nih.gov/pubmed/29925829 http://dx.doi.org/10.12659/MSM.908647 |
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