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The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence
BACKGROUND: Glioblastoma (GBM) has a high rate of local recurrence despite chemoradiotherapy (CRT). Genome-wide expression profiling was performed on patient tumors before and after chemoradiotherapy to identify genes and gene pathways associated with recurrence. RESULTS: Median time to recurrence w...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044384/ https://www.ncbi.nlm.nih.gov/pubmed/30018734 http://dx.doi.org/10.18632/oncotarget.25528 |
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author | Khwaja, Shariq S. Cai, Chunyu Badiyan, Shahed N. Wang, Xiaowei Huang, Jiayi |
author_facet | Khwaja, Shariq S. Cai, Chunyu Badiyan, Shahed N. Wang, Xiaowei Huang, Jiayi |
author_sort | Khwaja, Shariq S. |
collection | PubMed |
description | BACKGROUND: Glioblastoma (GBM) has a high rate of local recurrence despite chemoradiotherapy (CRT). Genome-wide expression profiling was performed on patient tumors before and after chemoradiotherapy to identify genes and gene pathways associated with recurrence. RESULTS: Median time to recurrence was 8.9 months with median time to second surgery of 9.6 months. The microRNA (miRNA) analysis identified 9 oncologic and immune-related miRNAs to be differentially expressed, including the hypoxia-related miR-210 and the immune-modulatory miR-146b. More than 1200 differentially-expressed genes were identified with RNA-sequencing (RNA-seq). Gene set enrichment analysis (GSEA) identified p53 signaling, Notch, Wnt, VEGF, and MEK gene sets enriched in recurrent GBM. Consistent with the miRNA profiling data, the miR-146b target gene set from GSEA analysis was also associated with recurrence. METHODS: Fourteen patients with GBM recurrence after CRT who had available tumor tissue from the initial diagnosis as well as recurrence were selected. Total RNA was isolated from formalin-fixed paraffin-embedded (FFPE) tumor specimens. Genome-wide expression profiling using RT-PCR for miRNA analysis and RNA-seq for messenger RNA (mRNA) analysis were conducted to identify differentially-expressed genes. GSEA was performed on the differential expression data. CONCLUSIONS: Genome-wide expression profiling identifies multiple oncologic and immune-related gene sets associated with GBM recurrence. In particular, immune-related miR-146b is upregulated in recurrence and deserves further investigation. |
format | Online Article Text |
id | pubmed-6044384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-60443842018-07-17 The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence Khwaja, Shariq S. Cai, Chunyu Badiyan, Shahed N. Wang, Xiaowei Huang, Jiayi Oncotarget Research Paper BACKGROUND: Glioblastoma (GBM) has a high rate of local recurrence despite chemoradiotherapy (CRT). Genome-wide expression profiling was performed on patient tumors before and after chemoradiotherapy to identify genes and gene pathways associated with recurrence. RESULTS: Median time to recurrence was 8.9 months with median time to second surgery of 9.6 months. The microRNA (miRNA) analysis identified 9 oncologic and immune-related miRNAs to be differentially expressed, including the hypoxia-related miR-210 and the immune-modulatory miR-146b. More than 1200 differentially-expressed genes were identified with RNA-sequencing (RNA-seq). Gene set enrichment analysis (GSEA) identified p53 signaling, Notch, Wnt, VEGF, and MEK gene sets enriched in recurrent GBM. Consistent with the miRNA profiling data, the miR-146b target gene set from GSEA analysis was also associated with recurrence. METHODS: Fourteen patients with GBM recurrence after CRT who had available tumor tissue from the initial diagnosis as well as recurrence were selected. Total RNA was isolated from formalin-fixed paraffin-embedded (FFPE) tumor specimens. Genome-wide expression profiling using RT-PCR for miRNA analysis and RNA-seq for messenger RNA (mRNA) analysis were conducted to identify differentially-expressed genes. GSEA was performed on the differential expression data. CONCLUSIONS: Genome-wide expression profiling identifies multiple oncologic and immune-related gene sets associated with GBM recurrence. In particular, immune-related miR-146b is upregulated in recurrence and deserves further investigation. Impact Journals LLC 2018-06-26 /pmc/articles/PMC6044384/ /pubmed/30018734 http://dx.doi.org/10.18632/oncotarget.25528 Text en Copyright: © 2018 Khwaja et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Khwaja, Shariq S. Cai, Chunyu Badiyan, Shahed N. Wang, Xiaowei Huang, Jiayi The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence |
title | The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence |
title_full | The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence |
title_fullStr | The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence |
title_full_unstemmed | The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence |
title_short | The immune-related microRNA miR-146b is upregulated in glioblastoma recurrence |
title_sort | immune-related microrna mir-146b is upregulated in glioblastoma recurrence |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044384/ https://www.ncbi.nlm.nih.gov/pubmed/30018734 http://dx.doi.org/10.18632/oncotarget.25528 |
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