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Artificial 3D Culture Systems for T Cell Expansion

[Image: see text] Adoptive cell therapy, i.e., the extraction, manipulation, and administration of ex vivo generated autologous T cells to patients, is an emerging alternative to regular procedures in cancer treatment. Nevertheless, these personalized treatments require laborious and expensive labor...

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Autores principales: Pérez del Río, Eduardo, Martinez Miguel, Marc, Veciana, Jaume, Ratera, Imma, Guasch, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044561/
https://www.ncbi.nlm.nih.gov/pubmed/30023914
http://dx.doi.org/10.1021/acsomega.8b00521
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author Pérez del Río, Eduardo
Martinez Miguel, Marc
Veciana, Jaume
Ratera, Imma
Guasch, Judith
author_facet Pérez del Río, Eduardo
Martinez Miguel, Marc
Veciana, Jaume
Ratera, Imma
Guasch, Judith
author_sort Pérez del Río, Eduardo
collection PubMed
description [Image: see text] Adoptive cell therapy, i.e., the extraction, manipulation, and administration of ex vivo generated autologous T cells to patients, is an emerging alternative to regular procedures in cancer treatment. Nevertheless, these personalized treatments require laborious and expensive laboratory procedures that should be alleviated to enable their incorporation into the clinics. With the objective to improve the ex vivo expansion of large amount of specific T cells, we propose the use of three-dimensional (3D) structures during their activation with artificial antigen-presenting cells, thus resembling the natural environment of the secondary lymphoid organs. Thus, the activation, proliferation, and differentiation of T cells have been analyzed when cultured in the presence of two 3D systems, Matrigel and a 3D polystyrene scaffold, showing an increase in cell proliferation compared to standard suspension systems.
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spelling pubmed-60445612018-07-16 Artificial 3D Culture Systems for T Cell Expansion Pérez del Río, Eduardo Martinez Miguel, Marc Veciana, Jaume Ratera, Imma Guasch, Judith ACS Omega [Image: see text] Adoptive cell therapy, i.e., the extraction, manipulation, and administration of ex vivo generated autologous T cells to patients, is an emerging alternative to regular procedures in cancer treatment. Nevertheless, these personalized treatments require laborious and expensive laboratory procedures that should be alleviated to enable their incorporation into the clinics. With the objective to improve the ex vivo expansion of large amount of specific T cells, we propose the use of three-dimensional (3D) structures during their activation with artificial antigen-presenting cells, thus resembling the natural environment of the secondary lymphoid organs. Thus, the activation, proliferation, and differentiation of T cells have been analyzed when cultured in the presence of two 3D systems, Matrigel and a 3D polystyrene scaffold, showing an increase in cell proliferation compared to standard suspension systems. American Chemical Society 2018-05-16 /pmc/articles/PMC6044561/ /pubmed/30023914 http://dx.doi.org/10.1021/acsomega.8b00521 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Pérez del Río, Eduardo
Martinez Miguel, Marc
Veciana, Jaume
Ratera, Imma
Guasch, Judith
Artificial 3D Culture Systems for T Cell Expansion
title Artificial 3D Culture Systems for T Cell Expansion
title_full Artificial 3D Culture Systems for T Cell Expansion
title_fullStr Artificial 3D Culture Systems for T Cell Expansion
title_full_unstemmed Artificial 3D Culture Systems for T Cell Expansion
title_short Artificial 3D Culture Systems for T Cell Expansion
title_sort artificial 3d culture systems for t cell expansion
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044561/
https://www.ncbi.nlm.nih.gov/pubmed/30023914
http://dx.doi.org/10.1021/acsomega.8b00521
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