An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display

[Image: see text] RNA–protein interactions have a central role in the living world. In this article, we examined whether primitive peptides (30 residues) consisting of four types of amino acid (Gly, Ala, Asp, and Val) could interact with tRNA as a model of primitive RNAs in the RNA world. By in vitr...

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Autores principales: Kumachi, Shigefumi, Husimi, Yuzuru, Nemoto, Naoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2016
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044570/
https://www.ncbi.nlm.nih.gov/pubmed/30023471
http://dx.doi.org/10.1021/acsomega.6b00015
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author Kumachi, Shigefumi
Husimi, Yuzuru
Nemoto, Naoto
author_facet Kumachi, Shigefumi
Husimi, Yuzuru
Nemoto, Naoto
author_sort Kumachi, Shigefumi
collection PubMed
description [Image: see text] RNA–protein interactions have a central role in the living world. In this article, we examined whether primitive peptides (30 residues) consisting of four types of amino acid (Gly, Ala, Asp, and Val) could interact with tRNA as a model of primitive RNAs in the RNA world. By in vitro selection of binding peptides using the cDNA display method, a characteristic peptide was selected from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Interestingly, the selected peptide bound to a single-stranded region including a loop structure of an RNA molecule with some sequence specificity.
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spelling pubmed-60445702018-07-16 An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display Kumachi, Shigefumi Husimi, Yuzuru Nemoto, Naoto ACS Omega [Image: see text] RNA–protein interactions have a central role in the living world. In this article, we examined whether primitive peptides (30 residues) consisting of four types of amino acid (Gly, Ala, Asp, and Val) could interact with tRNA as a model of primitive RNAs in the RNA world. By in vitro selection of binding peptides using the cDNA display method, a characteristic peptide was selected from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Interestingly, the selected peptide bound to a single-stranded region including a loop structure of an RNA molecule with some sequence specificity. American Chemical Society 2016-07-06 /pmc/articles/PMC6044570/ /pubmed/30023471 http://dx.doi.org/10.1021/acsomega.6b00015 Text en Copyright © 2016 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kumachi, Shigefumi
Husimi, Yuzuru
Nemoto, Naoto
An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
title An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
title_full An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
title_fullStr An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
title_full_unstemmed An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
title_short An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
title_sort rna binding peptide consisting of four types of amino acid by in vitro selection using cdna display
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044570/
https://www.ncbi.nlm.nih.gov/pubmed/30023471
http://dx.doi.org/10.1021/acsomega.6b00015
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