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Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation

[Image: see text] A chemical printing method based on gold nanoparticle (AuNP)-assisted laser ablation has been developed. By rastering a thin layer of AuNPs coated on a rat kidney tissue section with a UV laser, biomolecules are extracted and immediately transferred/printed onto a supporting glass...

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Autores principales: Chau, Siu-Leung, Tang, Ho-Wai, Cheng, Yu-Hong, Lok, Chun-Nam, Ng, Kwan-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044615/
https://www.ncbi.nlm.nih.gov/pubmed/30023759
http://dx.doi.org/10.1021/acsomega.7b00385
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author Chau, Siu-Leung
Tang, Ho-Wai
Cheng, Yu-Hong
Lok, Chun-Nam
Ng, Kwan-Ming
author_facet Chau, Siu-Leung
Tang, Ho-Wai
Cheng, Yu-Hong
Lok, Chun-Nam
Ng, Kwan-Ming
author_sort Chau, Siu-Leung
collection PubMed
description [Image: see text] A chemical printing method based on gold nanoparticle (AuNP)-assisted laser ablation has been developed. By rastering a thin layer of AuNPs coated on a rat kidney tissue section with a UV laser, biomolecules are extracted and immediately transferred/printed onto a supporting glass substrate. The integrity of the printed sample is preserved, as revealed by imaging mass spectrometric analysis. By studying the mechanism of the extraction/printing process, transiently molten AuNPs were found to be involved in the process, as supported by the color and morphological changes of the AuNP thin film. The success of this molecular printing method was based on the efficient laser–nanomaterial interaction, that is, the strong photoabsorption, laser-induced heating, and phase-transition properties of the AuNPs. It is anticipated that the molecular printing method can be applied to perform site-specific printing, which extracts and transfers biochemicals from different regions of biological tissue sections to different types of supporting materials for subsequent biochemical analysis with the preservation of the original tissue samples.
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spelling pubmed-60446152018-07-16 Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation Chau, Siu-Leung Tang, Ho-Wai Cheng, Yu-Hong Lok, Chun-Nam Ng, Kwan-Ming ACS Omega [Image: see text] A chemical printing method based on gold nanoparticle (AuNP)-assisted laser ablation has been developed. By rastering a thin layer of AuNPs coated on a rat kidney tissue section with a UV laser, biomolecules are extracted and immediately transferred/printed onto a supporting glass substrate. The integrity of the printed sample is preserved, as revealed by imaging mass spectrometric analysis. By studying the mechanism of the extraction/printing process, transiently molten AuNPs were found to be involved in the process, as supported by the color and morphological changes of the AuNP thin film. The success of this molecular printing method was based on the efficient laser–nanomaterial interaction, that is, the strong photoabsorption, laser-induced heating, and phase-transition properties of the AuNPs. It is anticipated that the molecular printing method can be applied to perform site-specific printing, which extracts and transfers biochemicals from different regions of biological tissue sections to different types of supporting materials for subsequent biochemical analysis with the preservation of the original tissue samples. American Chemical Society 2017-09-21 /pmc/articles/PMC6044615/ /pubmed/30023759 http://dx.doi.org/10.1021/acsomega.7b00385 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Chau, Siu-Leung
Tang, Ho-Wai
Cheng, Yu-Hong
Lok, Chun-Nam
Ng, Kwan-Ming
Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation
title Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation
title_full Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation
title_fullStr Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation
title_full_unstemmed Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation
title_short Chemical Printing of Biological Tissue by Gold Nanoparticle-Assisted Laser Ablation
title_sort chemical printing of biological tissue by gold nanoparticle-assisted laser ablation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044615/
https://www.ncbi.nlm.nih.gov/pubmed/30023759
http://dx.doi.org/10.1021/acsomega.7b00385
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