Charge-Driven Interaction of Antimicrobial Peptide NK-2 with Phospholipid Membranes

[Image: see text] NK-2, derived from a cationic core region of NK-lysin, displays antimicrobial activity toward negatively charged bacterial membranes. We have studied the interaction of NK-2 with various phospholipid membranes, using a variety of experimental techniques, such as, isothermal titration calorimetry (ITC), ζ potential, and dynamic light scattering. As bacteria mimicking membranes, we have chosen large unilamellar vesicles (LUVs) composed of negatively charged phospholipid and neutral phospholipids. ITC and ζ potential results show the stronger binding affinity of NK-2 to negatively charged membranes than to neutral membranes. Saturation of the isotherm, obtained from ITC, at a given lipid to NK-2 ratio, was found to be consistent with the charge compensation, determined from ζ potential. A surface partition model with electrostatic contribution was used to estimate the intrinsic binding constant and other thermodynamical parameters of binding kinetics of NK-2. The size distribution of negatively charged LUV in the presence of NK-2 was found to increase drastically, indicating the presence of large aggregates. Such a large aggregate has not been observed in neutral membranes, which supports the ITC and ζ potential results.