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Evaluation of Silk Inverse Opals for “Smart” Tissue Culture

[Image: see text] Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to transduce both the optical inf...

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Autores principales: Tseng, Peter, Zhao, Siwei, Golding, Annie, Applegate, Matthew B., Mitropoulos, Alexander N., Kaplan, David L., Omenetto, Fiorenzo G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044746/
https://www.ncbi.nlm.nih.gov/pubmed/30023608
http://dx.doi.org/10.1021/acsomega.6b00320
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author Tseng, Peter
Zhao, Siwei
Golding, Annie
Applegate, Matthew B.
Mitropoulos, Alexander N.
Kaplan, David L.
Omenetto, Fiorenzo G.
author_facet Tseng, Peter
Zhao, Siwei
Golding, Annie
Applegate, Matthew B.
Mitropoulos, Alexander N.
Kaplan, David L.
Omenetto, Fiorenzo G.
author_sort Tseng, Peter
collection PubMed
description [Image: see text] Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to transduce both the optical information and cells during tissue culture. We show that these substrates can visually track substrate degradation in various proteases during tissue digestion and protein deposition during the growth of mesenchymal stem cells. Uniquely, these substrates can be integrated in multiple steps of tissue culture for simple-to-use, visual, and quantitative detectors of bioactivity. These substrates can also be doped, demonstrated here with gold nanoparticles, to allow additional control of cell functions.
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spelling pubmed-60447462018-07-16 Evaluation of Silk Inverse Opals for “Smart” Tissue Culture Tseng, Peter Zhao, Siwei Golding, Annie Applegate, Matthew B. Mitropoulos, Alexander N. Kaplan, David L. Omenetto, Fiorenzo G. ACS Omega [Image: see text] Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to transduce both the optical information and cells during tissue culture. We show that these substrates can visually track substrate degradation in various proteases during tissue digestion and protein deposition during the growth of mesenchymal stem cells. Uniquely, these substrates can be integrated in multiple steps of tissue culture for simple-to-use, visual, and quantitative detectors of bioactivity. These substrates can also be doped, demonstrated here with gold nanoparticles, to allow additional control of cell functions. American Chemical Society 2017-02-10 /pmc/articles/PMC6044746/ /pubmed/30023608 http://dx.doi.org/10.1021/acsomega.6b00320 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Tseng, Peter
Zhao, Siwei
Golding, Annie
Applegate, Matthew B.
Mitropoulos, Alexander N.
Kaplan, David L.
Omenetto, Fiorenzo G.
Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
title Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
title_full Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
title_fullStr Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
title_full_unstemmed Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
title_short Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
title_sort evaluation of silk inverse opals for “smart” tissue culture
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044746/
https://www.ncbi.nlm.nih.gov/pubmed/30023608
http://dx.doi.org/10.1021/acsomega.6b00320
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