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Evaluation of Silk Inverse Opals for “Smart” Tissue Culture
[Image: see text] Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to transduce both the optical inf...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044746/ https://www.ncbi.nlm.nih.gov/pubmed/30023608 http://dx.doi.org/10.1021/acsomega.6b00320 |
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author | Tseng, Peter Zhao, Siwei Golding, Annie Applegate, Matthew B. Mitropoulos, Alexander N. Kaplan, David L. Omenetto, Fiorenzo G. |
author_facet | Tseng, Peter Zhao, Siwei Golding, Annie Applegate, Matthew B. Mitropoulos, Alexander N. Kaplan, David L. Omenetto, Fiorenzo G. |
author_sort | Tseng, Peter |
collection | PubMed |
description | [Image: see text] Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to transduce both the optical information and cells during tissue culture. We show that these substrates can visually track substrate degradation in various proteases during tissue digestion and protein deposition during the growth of mesenchymal stem cells. Uniquely, these substrates can be integrated in multiple steps of tissue culture for simple-to-use, visual, and quantitative detectors of bioactivity. These substrates can also be doped, demonstrated here with gold nanoparticles, to allow additional control of cell functions. |
format | Online Article Text |
id | pubmed-6044746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60447462018-07-16 Evaluation of Silk Inverse Opals for “Smart” Tissue Culture Tseng, Peter Zhao, Siwei Golding, Annie Applegate, Matthew B. Mitropoulos, Alexander N. Kaplan, David L. Omenetto, Fiorenzo G. ACS Omega [Image: see text] Visually tracking the subtle aspects of biological systems in real time during tissue culture remains challenging. Herein, we demonstrate the use of bioactive, cytocompatible, and biodegradable inverse opals from silk as a multifunctional substrate to transduce both the optical information and cells during tissue culture. We show that these substrates can visually track substrate degradation in various proteases during tissue digestion and protein deposition during the growth of mesenchymal stem cells. Uniquely, these substrates can be integrated in multiple steps of tissue culture for simple-to-use, visual, and quantitative detectors of bioactivity. These substrates can also be doped, demonstrated here with gold nanoparticles, to allow additional control of cell functions. American Chemical Society 2017-02-10 /pmc/articles/PMC6044746/ /pubmed/30023608 http://dx.doi.org/10.1021/acsomega.6b00320 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Tseng, Peter Zhao, Siwei Golding, Annie Applegate, Matthew B. Mitropoulos, Alexander N. Kaplan, David L. Omenetto, Fiorenzo G. Evaluation of Silk Inverse Opals for “Smart” Tissue Culture |
title | Evaluation of Silk Inverse Opals for “Smart”
Tissue Culture |
title_full | Evaluation of Silk Inverse Opals for “Smart”
Tissue Culture |
title_fullStr | Evaluation of Silk Inverse Opals for “Smart”
Tissue Culture |
title_full_unstemmed | Evaluation of Silk Inverse Opals for “Smart”
Tissue Culture |
title_short | Evaluation of Silk Inverse Opals for “Smart”
Tissue Culture |
title_sort | evaluation of silk inverse opals for “smart”
tissue culture |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044746/ https://www.ncbi.nlm.nih.gov/pubmed/30023608 http://dx.doi.org/10.1021/acsomega.6b00320 |
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