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Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2

[Image: see text] The development of novel materials capable of delivering multiple growth factors is urgent and essential for rapid and effective tissue regeneration. In this study, a kind of composite film composed of poly-l-lysine (PLL), heparin (Hep), and Au nanoparitcles (Au nps) has been fabri...

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Autores principales: Qi, Wei, Yan, Jing, Sun, Haifeng, Wang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044765/
https://www.ncbi.nlm.nih.gov/pubmed/30023619
http://dx.doi.org/10.1021/acsomega.6b00420
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author Qi, Wei
Yan, Jing
Sun, Haifeng
Wang, Hua
author_facet Qi, Wei
Yan, Jing
Sun, Haifeng
Wang, Hua
author_sort Qi, Wei
collection PubMed
description [Image: see text] The development of novel materials capable of delivering multiple growth factors is urgent and essential for rapid and effective tissue regeneration. In this study, a kind of composite film composed of poly-l-lysine (PLL), heparin (Hep), and Au nanoparitcles (Au nps) has been fabricated to deliver the basic fibroblast growth factor (bFGF) and bone morphogenetic protein-2 (BMP-2) simultaneously. The films have been found to show enhanced mechanical property due to the incorporation of Au nps. They have also shown good anticoagulation activity with long activated partial thromboplastin time because of the contribution of Hep molecules. Moreover, the osteogenesis studies reveal that the loaded bFGF and BMP-2 in the composite films have a synergistic differentiation effect on mesenchymal stem cells, as indicated by alkaline phosphatase (ALP) activity assay and collagen type I (Col-I) gene expression. In contrast to the (PLL/Hep)(6)/BMP-2/(PLL/Au nps)(6)/(PLL/Hep)(6) and (PLL/Hep)(6)/(PLL/Au nps)(6)/(PLL/Hep)(6)/bFGF films, the (PLL/Hep)(6)/BMP-2/(PLL/Au nps)(6)/(PLL/Hep)(6)/bFGF films have shown higher ALP activity and higher Col-I expression level. Therefore, the developed multifunctional films could be potentially used as osteoinductive coatings of biomaterials. Particularly, this simple and convenient strategy provides an effective approach for the immobilization of multiple growth factors, which may be extended to other bioactive systems for the development of novel multifunctional bioactive surfaces.
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spelling pubmed-60447652018-07-16 Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2 Qi, Wei Yan, Jing Sun, Haifeng Wang, Hua ACS Omega [Image: see text] The development of novel materials capable of delivering multiple growth factors is urgent and essential for rapid and effective tissue regeneration. In this study, a kind of composite film composed of poly-l-lysine (PLL), heparin (Hep), and Au nanoparitcles (Au nps) has been fabricated to deliver the basic fibroblast growth factor (bFGF) and bone morphogenetic protein-2 (BMP-2) simultaneously. The films have been found to show enhanced mechanical property due to the incorporation of Au nps. They have also shown good anticoagulation activity with long activated partial thromboplastin time because of the contribution of Hep molecules. Moreover, the osteogenesis studies reveal that the loaded bFGF and BMP-2 in the composite films have a synergistic differentiation effect on mesenchymal stem cells, as indicated by alkaline phosphatase (ALP) activity assay and collagen type I (Col-I) gene expression. In contrast to the (PLL/Hep)(6)/BMP-2/(PLL/Au nps)(6)/(PLL/Hep)(6) and (PLL/Hep)(6)/(PLL/Au nps)(6)/(PLL/Hep)(6)/bFGF films, the (PLL/Hep)(6)/BMP-2/(PLL/Au nps)(6)/(PLL/Hep)(6)/bFGF films have shown higher ALP activity and higher Col-I expression level. Therefore, the developed multifunctional films could be potentially used as osteoinductive coatings of biomaterials. Particularly, this simple and convenient strategy provides an effective approach for the immobilization of multiple growth factors, which may be extended to other bioactive systems for the development of novel multifunctional bioactive surfaces. American Chemical Society 2017-03-14 /pmc/articles/PMC6044765/ /pubmed/30023619 http://dx.doi.org/10.1021/acsomega.6b00420 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Qi, Wei
Yan, Jing
Sun, Haifeng
Wang, Hua
Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2
title Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2
title_full Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2
title_fullStr Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2
title_full_unstemmed Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2
title_short Multifunctional Nanocomposite Films for Synergistic Delivery of bFGF and BMP-2
title_sort multifunctional nanocomposite films for synergistic delivery of bfgf and bmp-2
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044765/
https://www.ncbi.nlm.nih.gov/pubmed/30023619
http://dx.doi.org/10.1021/acsomega.6b00420
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