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Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
[Image: see text] Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the poly...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044838/ https://www.ncbi.nlm.nih.gov/pubmed/30023545 http://dx.doi.org/10.1021/acsomega.7b00700 |
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author | Kreuzer, Lucas Philipp Männel, Max Julius Schubert, Jonas Höller, Roland P. M. Chanana, Munish |
author_facet | Kreuzer, Lucas Philipp Männel, Max Julius Schubert, Jonas Höller, Roland P. M. Chanana, Munish |
author_sort | Kreuzer, Lucas Philipp |
collection | PubMed |
description | [Image: see text] Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the polymer product. Here, we present a compelling approach for the separation of the two macromolecular species, by taking the catalyst out of the molecular domain and locating it in the colloidal domain, ensuring quasi-homogeneous catalysis as well as easy separation of precious biocatalysts. We report on gold nanoparticles coated with horseradish peroxidase that can catalyze the polymerization of various monomers (e.g., N-isopropylacrylamide), yielding thermoresponsive polymers. Strikingly, these biocatalyst-coated nanoparticles can be recovered completely and reused in more than three independent polymerization cycles, without significant loss of their catalytic activity. |
format | Online Article Text |
id | pubmed-6044838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60448382018-07-16 Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions Kreuzer, Lucas Philipp Männel, Max Julius Schubert, Jonas Höller, Roland P. M. Chanana, Munish ACS Omega [Image: see text] Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the polymer product. Here, we present a compelling approach for the separation of the two macromolecular species, by taking the catalyst out of the molecular domain and locating it in the colloidal domain, ensuring quasi-homogeneous catalysis as well as easy separation of precious biocatalysts. We report on gold nanoparticles coated with horseradish peroxidase that can catalyze the polymerization of various monomers (e.g., N-isopropylacrylamide), yielding thermoresponsive polymers. Strikingly, these biocatalyst-coated nanoparticles can be recovered completely and reused in more than three independent polymerization cycles, without significant loss of their catalytic activity. American Chemical Society 2017-10-27 /pmc/articles/PMC6044838/ /pubmed/30023545 http://dx.doi.org/10.1021/acsomega.7b00700 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Kreuzer, Lucas Philipp Männel, Max Julius Schubert, Jonas Höller, Roland P. M. Chanana, Munish Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions |
title | Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles
as Colloidal Biocatalysts for Polymerization Reactions |
title_full | Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles
as Colloidal Biocatalysts for Polymerization Reactions |
title_fullStr | Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles
as Colloidal Biocatalysts for Polymerization Reactions |
title_full_unstemmed | Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles
as Colloidal Biocatalysts for Polymerization Reactions |
title_short | Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles
as Colloidal Biocatalysts for Polymerization Reactions |
title_sort | enzymatic catalysis at nanoscale: enzyme-coated nanoparticles
as colloidal biocatalysts for polymerization reactions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044838/ https://www.ncbi.nlm.nih.gov/pubmed/30023545 http://dx.doi.org/10.1021/acsomega.7b00700 |
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