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Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions

[Image: see text] Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the poly...

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Autores principales: Kreuzer, Lucas Philipp, Männel, Max Julius, Schubert, Jonas, Höller, Roland P. M., Chanana, Munish
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044838/
https://www.ncbi.nlm.nih.gov/pubmed/30023545
http://dx.doi.org/10.1021/acsomega.7b00700
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author Kreuzer, Lucas Philipp
Männel, Max Julius
Schubert, Jonas
Höller, Roland P. M.
Chanana, Munish
author_facet Kreuzer, Lucas Philipp
Männel, Max Julius
Schubert, Jonas
Höller, Roland P. M.
Chanana, Munish
author_sort Kreuzer, Lucas Philipp
collection PubMed
description [Image: see text] Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the polymer product. Here, we present a compelling approach for the separation of the two macromolecular species, by taking the catalyst out of the molecular domain and locating it in the colloidal domain, ensuring quasi-homogeneous catalysis as well as easy separation of precious biocatalysts. We report on gold nanoparticles coated with horseradish peroxidase that can catalyze the polymerization of various monomers (e.g., N-isopropylacrylamide), yielding thermoresponsive polymers. Strikingly, these biocatalyst-coated nanoparticles can be recovered completely and reused in more than three independent polymerization cycles, without significant loss of their catalytic activity.
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spelling pubmed-60448382018-07-16 Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions Kreuzer, Lucas Philipp Männel, Max Julius Schubert, Jonas Höller, Roland P. M. Chanana, Munish ACS Omega [Image: see text] Enzyme-catalyzed controlled radical polymerization represents a powerful approach for the polymerization of a wide variety of water-soluble monomers. However, in such an enzyme-based polymerization system, the macromolecular catalyst (i.e., enzyme) has to be separated from the polymer product. Here, we present a compelling approach for the separation of the two macromolecular species, by taking the catalyst out of the molecular domain and locating it in the colloidal domain, ensuring quasi-homogeneous catalysis as well as easy separation of precious biocatalysts. We report on gold nanoparticles coated with horseradish peroxidase that can catalyze the polymerization of various monomers (e.g., N-isopropylacrylamide), yielding thermoresponsive polymers. Strikingly, these biocatalyst-coated nanoparticles can be recovered completely and reused in more than three independent polymerization cycles, without significant loss of their catalytic activity. American Chemical Society 2017-10-27 /pmc/articles/PMC6044838/ /pubmed/30023545 http://dx.doi.org/10.1021/acsomega.7b00700 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kreuzer, Lucas Philipp
Männel, Max Julius
Schubert, Jonas
Höller, Roland P. M.
Chanana, Munish
Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
title Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
title_full Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
title_fullStr Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
title_full_unstemmed Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
title_short Enzymatic Catalysis at Nanoscale: Enzyme-Coated Nanoparticles as Colloidal Biocatalysts for Polymerization Reactions
title_sort enzymatic catalysis at nanoscale: enzyme-coated nanoparticles as colloidal biocatalysts for polymerization reactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044838/
https://www.ncbi.nlm.nih.gov/pubmed/30023545
http://dx.doi.org/10.1021/acsomega.7b00700
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