Cargando…
Microencapsulation of Live Cells in Synthetic Polymer Capsules
[Image: see text] In cell therapies, it is advantageous to encapsulate live cells in protective, semipermeable microparticles for controlled release of cytokines, growth factors, monoclonal antibodies, or insulin. Here, a modified electrospraying approach with an organic solution of poly(lactide-co-...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044854/ https://www.ncbi.nlm.nih.gov/pubmed/30023677 http://dx.doi.org/10.1021/acsomega.7b00570 |
_version_ | 1783339557927780352 |
---|---|
author | Esfahani, Reza Roghani Jun, Haysun Rahmani, Sahar Miller, Andrea Lahann, Joerg |
author_facet | Esfahani, Reza Roghani Jun, Haysun Rahmani, Sahar Miller, Andrea Lahann, Joerg |
author_sort | Esfahani, Reza Roghani |
collection | PubMed |
description | [Image: see text] In cell therapies, it is advantageous to encapsulate live cells in protective, semipermeable microparticles for controlled release of cytokines, growth factors, monoclonal antibodies, or insulin. Here, a modified electrospraying approach with an organic solution of poly(lactide-co-glycolide) (PLGA) polymer is used to create synthetic PLGA capsules that effectively protect live cells. Using a design of experiment (DOE) methodology, the effect of governing jetting parameters on encapsulation efficiency, yield, and size is systematically evaluated. On the basis of this analysis, the interaction between bovine serum albumin concentration and core flow rate is the most dominant factor determining core encapsulation efficiency as well as the microcapsule size. However, the interaction between shell solvent ratio and shell flow rate predominantly defines the particle yield. To validate these findings, live cells have been successfully encapsulated in microcapsules using optimized parameters from the DOE analysis and have survived the electrohydrodynamic jetting process. Extending the currently available toolkit for cell microencapsulation, these biodegradable, semi-impermeable cell-laden microcapsules may find a range of applications in areas such as tissue engineering, regenerative medicine, and drug delivery. |
format | Online Article Text |
id | pubmed-6044854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60448542018-07-16 Microencapsulation of Live Cells in Synthetic Polymer Capsules Esfahani, Reza Roghani Jun, Haysun Rahmani, Sahar Miller, Andrea Lahann, Joerg ACS Omega [Image: see text] In cell therapies, it is advantageous to encapsulate live cells in protective, semipermeable microparticles for controlled release of cytokines, growth factors, monoclonal antibodies, or insulin. Here, a modified electrospraying approach with an organic solution of poly(lactide-co-glycolide) (PLGA) polymer is used to create synthetic PLGA capsules that effectively protect live cells. Using a design of experiment (DOE) methodology, the effect of governing jetting parameters on encapsulation efficiency, yield, and size is systematically evaluated. On the basis of this analysis, the interaction between bovine serum albumin concentration and core flow rate is the most dominant factor determining core encapsulation efficiency as well as the microcapsule size. However, the interaction between shell solvent ratio and shell flow rate predominantly defines the particle yield. To validate these findings, live cells have been successfully encapsulated in microcapsules using optimized parameters from the DOE analysis and have survived the electrohydrodynamic jetting process. Extending the currently available toolkit for cell microencapsulation, these biodegradable, semi-impermeable cell-laden microcapsules may find a range of applications in areas such as tissue engineering, regenerative medicine, and drug delivery. American Chemical Society 2017-06-21 /pmc/articles/PMC6044854/ /pubmed/30023677 http://dx.doi.org/10.1021/acsomega.7b00570 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Esfahani, Reza Roghani Jun, Haysun Rahmani, Sahar Miller, Andrea Lahann, Joerg Microencapsulation of Live Cells in Synthetic Polymer Capsules |
title | Microencapsulation of Live Cells in Synthetic Polymer
Capsules |
title_full | Microencapsulation of Live Cells in Synthetic Polymer
Capsules |
title_fullStr | Microencapsulation of Live Cells in Synthetic Polymer
Capsules |
title_full_unstemmed | Microencapsulation of Live Cells in Synthetic Polymer
Capsules |
title_short | Microencapsulation of Live Cells in Synthetic Polymer
Capsules |
title_sort | microencapsulation of live cells in synthetic polymer
capsules |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044854/ https://www.ncbi.nlm.nih.gov/pubmed/30023677 http://dx.doi.org/10.1021/acsomega.7b00570 |
work_keys_str_mv | AT esfahanirezaroghani microencapsulationoflivecellsinsyntheticpolymercapsules AT junhaysun microencapsulationoflivecellsinsyntheticpolymercapsules AT rahmanisahar microencapsulationoflivecellsinsyntheticpolymercapsules AT millerandrea microencapsulationoflivecellsinsyntheticpolymercapsules AT lahannjoerg microencapsulationoflivecellsinsyntheticpolymercapsules |