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Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses
[Image: see text] B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% b...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044859/ https://www.ncbi.nlm.nih.gov/pubmed/30023708 http://dx.doi.org/10.1021/acsomega.7b00608 |
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author | Freire, Marjorie C. L. C. Pol-Fachin, Laércio Coêlho, Danilo F. Viana, Isabelle F. T. Magalhães, Tereza Cordeiro, Marli T. Fischer, Nico Loeffler, Felix F. Jaenisch, Thomas Franca, Rafael F. Marques, Ernesto T. A. Lins, Roberto D. |
author_facet | Freire, Marjorie C. L. C. Pol-Fachin, Laércio Coêlho, Danilo F. Viana, Isabelle F. T. Magalhães, Tereza Cordeiro, Marli T. Fischer, Nico Loeffler, Felix F. Jaenisch, Thomas Franca, Rafael F. Marques, Ernesto T. A. Lins, Roberto D. |
author_sort | Freire, Marjorie C. L. C. |
collection | PubMed |
description | [Image: see text] B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% between the NS1 from ZIKV and the four dengue virus (DENV) serotypes, epitope sequences were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for a dramatically divergent electrostatic surface potential on the epitope regions of ZIKV and DENV2 serotypes. These findings suggest that strategies for differential diagnostics on the basis of short linear NS1 sequences are likely to fail due to immunological cross-reactions. Overall, results provide the molecular basis of differential discrimination between Zika and DENVs by NS1 monoclonal antibodies. |
format | Online Article Text |
id | pubmed-6044859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60448592018-07-16 Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses Freire, Marjorie C. L. C. Pol-Fachin, Laércio Coêlho, Danilo F. Viana, Isabelle F. T. Magalhães, Tereza Cordeiro, Marli T. Fischer, Nico Loeffler, Felix F. Jaenisch, Thomas Franca, Rafael F. Marques, Ernesto T. A. Lins, Roberto D. ACS Omega [Image: see text] B-cell epitope sequences from Zika virus (ZIKV) NS1 protein have been identified using epitope prediction tools. Mapping these sequences onto the NS1 surface reveals two major conformational epitopes and a single linear one. Despite an overall average sequence identity of ca. 55% between the NS1 from ZIKV and the four dengue virus (DENV) serotypes, epitope sequences were found to be highly conserved. Nevertheless, nonconserved epitope-flanking residues are responsible for a dramatically divergent electrostatic surface potential on the epitope regions of ZIKV and DENV2 serotypes. These findings suggest that strategies for differential diagnostics on the basis of short linear NS1 sequences are likely to fail due to immunological cross-reactions. Overall, results provide the molecular basis of differential discrimination between Zika and DENVs by NS1 monoclonal antibodies. American Chemical Society 2017-07-25 /pmc/articles/PMC6044859/ /pubmed/30023708 http://dx.doi.org/10.1021/acsomega.7b00608 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Freire, Marjorie C. L. C. Pol-Fachin, Laércio Coêlho, Danilo F. Viana, Isabelle F. T. Magalhães, Tereza Cordeiro, Marli T. Fischer, Nico Loeffler, Felix F. Jaenisch, Thomas Franca, Rafael F. Marques, Ernesto T. A. Lins, Roberto D. Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses |
title | Mapping Putative B-Cell Zika Virus NS1 Epitopes
Provides Molecular Basis for Anti-NS1 Antibody Discrimination between
Zika and Dengue Viruses |
title_full | Mapping Putative B-Cell Zika Virus NS1 Epitopes
Provides Molecular Basis for Anti-NS1 Antibody Discrimination between
Zika and Dengue Viruses |
title_fullStr | Mapping Putative B-Cell Zika Virus NS1 Epitopes
Provides Molecular Basis for Anti-NS1 Antibody Discrimination between
Zika and Dengue Viruses |
title_full_unstemmed | Mapping Putative B-Cell Zika Virus NS1 Epitopes
Provides Molecular Basis for Anti-NS1 Antibody Discrimination between
Zika and Dengue Viruses |
title_short | Mapping Putative B-Cell Zika Virus NS1 Epitopes
Provides Molecular Basis for Anti-NS1 Antibody Discrimination between
Zika and Dengue Viruses |
title_sort | mapping putative b-cell zika virus ns1 epitopes
provides molecular basis for anti-ns1 antibody discrimination between
zika and dengue viruses |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044859/ https://www.ncbi.nlm.nih.gov/pubmed/30023708 http://dx.doi.org/10.1021/acsomega.7b00608 |
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