From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative

[Image: see text] The current article describes the synthesis, characterization, and application of a designed hybrid fluorescent BTD–coumarin (2,1,3-benzothiadiazole-coumarin) derivative (named BTD-Lip). The use of BTD-Lip for live-cells staining showed excellent results, and lipid droplets (LDs) c...

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Autores principales: Mota, Alberto A. R., Correa, Jose R., de Andrade, Lorena P., Assumpção, José A. F., de Souza Cintra, Giovana A., Freitas-Junior, Lucio H., da Silva, Wender A., de Oliveira, Heibbe C. B., Neto, Brenno A. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044862/
https://www.ncbi.nlm.nih.gov/pubmed/30023883
http://dx.doi.org/10.1021/acsomega.8b00434
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author Mota, Alberto A. R.
Correa, Jose R.
de Andrade, Lorena P.
Assumpção, José A. F.
de Souza Cintra, Giovana A.
Freitas-Junior, Lucio H.
da Silva, Wender A.
de Oliveira, Heibbe C. B.
Neto, Brenno A. D.
author_facet Mota, Alberto A. R.
Correa, Jose R.
de Andrade, Lorena P.
Assumpção, José A. F.
de Souza Cintra, Giovana A.
Freitas-Junior, Lucio H.
da Silva, Wender A.
de Oliveira, Heibbe C. B.
Neto, Brenno A. D.
author_sort Mota, Alberto A. R.
collection PubMed
description [Image: see text] The current article describes the synthesis, characterization, and application of a designed hybrid fluorescent BTD–coumarin (2,1,3-benzothiadiazole-coumarin) derivative (named BTD-Lip). The use of BTD-Lip for live-cells staining showed excellent results, and lipid droplets (LDs) could be selectively stained. When compared with the commercially available dye (BODIPY) for LD staining, it was noted that the designed hybrid fluorescence was capable of staining a considerable larger number of LDs in both live and fixed cells (ca. 40% more). The new dye was also tested on live Caenorhabditis elegans (complex model) and showed an impressive selectivity inside the worm, whereas the commercial dye showed no selectivity in the complex model.
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spelling pubmed-60448622018-07-16 From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative Mota, Alberto A. R. Correa, Jose R. de Andrade, Lorena P. Assumpção, José A. F. de Souza Cintra, Giovana A. Freitas-Junior, Lucio H. da Silva, Wender A. de Oliveira, Heibbe C. B. Neto, Brenno A. D. ACS Omega [Image: see text] The current article describes the synthesis, characterization, and application of a designed hybrid fluorescent BTD–coumarin (2,1,3-benzothiadiazole-coumarin) derivative (named BTD-Lip). The use of BTD-Lip for live-cells staining showed excellent results, and lipid droplets (LDs) could be selectively stained. When compared with the commercially available dye (BODIPY) for LD staining, it was noted that the designed hybrid fluorescence was capable of staining a considerable larger number of LDs in both live and fixed cells (ca. 40% more). The new dye was also tested on live Caenorhabditis elegans (complex model) and showed an impressive selectivity inside the worm, whereas the commercial dye showed no selectivity in the complex model. American Chemical Society 2018-04-05 /pmc/articles/PMC6044862/ /pubmed/30023883 http://dx.doi.org/10.1021/acsomega.8b00434 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Mota, Alberto A. R.
Correa, Jose R.
de Andrade, Lorena P.
Assumpção, José A. F.
de Souza Cintra, Giovana A.
Freitas-Junior, Lucio H.
da Silva, Wender A.
de Oliveira, Heibbe C. B.
Neto, Brenno A. D.
From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
title From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
title_full From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
title_fullStr From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
title_full_unstemmed From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
title_short From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative
title_sort from live cells to caenorhabditis elegans: selective staining and quantification of lipid structures using a fluorescent hybrid benzothiadiazole derivative
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044862/
https://www.ncbi.nlm.nih.gov/pubmed/30023883
http://dx.doi.org/10.1021/acsomega.8b00434
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