Facile and Green Synthesis of Multicolor Fluorescence Carbon Dots from Curcumin: In Vitro and in Vivo Bioimaging and Other Applications

[Image: see text] Early detection is the critical phase in the prognostic strategy of various life-threatening maladies like infectious diseases and cancer. The mortality rate caused by these diseases could be considerably reduced if they were diagnosed in the early stages of disease development. Carbon dots (C-dots), a relatively new and promising candidate in the fluorescent nanomaterial category, possess a perceptible impact on various bioapplications. Herein, we report a one-step facile hydrothermal synthesis that yields a novel surface-passivated carbon dot (CDP) from curcumin (as a green substrate) displaying high aqueous solubility. The physico-chemical characterization of thus synthesized C-dots was accomplished by an UV–visible spectrophotometer, fluorescence spectrophotometer, zetasizer, TEM, and FE-SEM to understand the formation of carbon dots with a 4–5 nm size near spherical nanoparticle with high colloidal stability. E. coli DH5α was engaged as the Gram-negative test organism and S. aureus as the Gram-positive in the biolabeling of bacteria. Cancer cell lines including colon cancer (HCT-15), lung cancer (A549), and mouse fibroblast (NIH 3T3) were evaluated and resulted in good biolabeling potential and less cytotoxicity. Zebrafish (ASWT) embryos as an animal model system were bioimaged, and in vivo toxicity was inferred. Moreover, the synthesized C-dots were shown to have free radical scavenging activity in a dose-dependent manner. The unpassivated C-dots (CD) were found to sense ferric ions at the micromolar concentration level. The findings of our study suggest that the multifunctional potentiality of CDPs serves as high-performance optical nanoprobes and can be a suitable alternative for various biolabeling and contrasting agents.