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Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells
[Image: see text] Herein, we report the surface functionality of dicationic cysteamine conjugated cholic acid (DCaC), dicationic cysteamine conjugated deoxycholic acid (DCaDC), and dicationic cysteamine conjugated lithocholic acid (DCaLC) templated gold nanoparticles (AuNPs) on mammalian cells. The...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044882/ https://www.ncbi.nlm.nih.gov/pubmed/30023697 http://dx.doi.org/10.1021/acsomega.7b00353 |
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author | Muthukumarasamyvel, Thangavel Rajendran, Ganapathy Santhana Panneer, Devendrapandi Kasthuri, Jayapalan Kathiravan, Krishnan Rajendiran, Nagappan |
author_facet | Muthukumarasamyvel, Thangavel Rajendran, Ganapathy Santhana Panneer, Devendrapandi Kasthuri, Jayapalan Kathiravan, Krishnan Rajendiran, Nagappan |
author_sort | Muthukumarasamyvel, Thangavel |
collection | PubMed |
description | [Image: see text] Herein, we report the surface functionality of dicationic cysteamine conjugated cholic acid (DCaC), dicationic cysteamine conjugated deoxycholic acid (DCaDC), and dicationic cysteamine conjugated lithocholic acid (DCaLC) templated gold nanoparticles (AuNPs) on mammalian cells. The haemocompatibility of the synthesized NPs was evaluated by in vitro hemolysis and erythrocyte sedimentation rate using human red blood cells (RBCs). In all of the systems, no toxicity was observed on human erythrocytes (RBCs) up to the concentration of 120 μg/mL. The anticancer activity of these dicationic amphiphile-stabilized AuNPs on A549 lung cancer cells was demonstrated by in vitro cell viability assay, intracellular reactive oxygen species estimation by DCFH-DA, apoptosis analysis using AO-EtBr fluorescence staining, DNA fragmentation analysis by agarose gel electrophoresis, and western blot analysis of caspase-3 expression. These results suggest that the cytotoxicity of AuNPs to A549 cells increase with the dose and hydrophobicity of amphiphiles and were found to be in the order: DCaLC-AuNPs > DCaDC-AuNPs > DCaC-AuNPs. |
format | Online Article Text |
id | pubmed-6044882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60448822018-07-16 Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells Muthukumarasamyvel, Thangavel Rajendran, Ganapathy Santhana Panneer, Devendrapandi Kasthuri, Jayapalan Kathiravan, Krishnan Rajendiran, Nagappan ACS Omega [Image: see text] Herein, we report the surface functionality of dicationic cysteamine conjugated cholic acid (DCaC), dicationic cysteamine conjugated deoxycholic acid (DCaDC), and dicationic cysteamine conjugated lithocholic acid (DCaLC) templated gold nanoparticles (AuNPs) on mammalian cells. The haemocompatibility of the synthesized NPs was evaluated by in vitro hemolysis and erythrocyte sedimentation rate using human red blood cells (RBCs). In all of the systems, no toxicity was observed on human erythrocytes (RBCs) up to the concentration of 120 μg/mL. The anticancer activity of these dicationic amphiphile-stabilized AuNPs on A549 lung cancer cells was demonstrated by in vitro cell viability assay, intracellular reactive oxygen species estimation by DCFH-DA, apoptosis analysis using AO-EtBr fluorescence staining, DNA fragmentation analysis by agarose gel electrophoresis, and western blot analysis of caspase-3 expression. These results suggest that the cytotoxicity of AuNPs to A549 cells increase with the dose and hydrophobicity of amphiphiles and were found to be in the order: DCaLC-AuNPs > DCaDC-AuNPs > DCaC-AuNPs. American Chemical Society 2017-07-25 /pmc/articles/PMC6044882/ /pubmed/30023697 http://dx.doi.org/10.1021/acsomega.7b00353 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Muthukumarasamyvel, Thangavel Rajendran, Ganapathy Santhana Panneer, Devendrapandi Kasthuri, Jayapalan Kathiravan, Krishnan Rajendiran, Nagappan Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells |
title | Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized
Gold Nanoparticles on A549 Lung Cancer Cells |
title_full | Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized
Gold Nanoparticles on A549 Lung Cancer Cells |
title_fullStr | Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized
Gold Nanoparticles on A549 Lung Cancer Cells |
title_full_unstemmed | Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized
Gold Nanoparticles on A549 Lung Cancer Cells |
title_short | Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized
Gold Nanoparticles on A549 Lung Cancer Cells |
title_sort | role of surface hydrophobicity of dicationic amphiphile-stabilized
gold nanoparticles on a549 lung cancer cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044882/ https://www.ncbi.nlm.nih.gov/pubmed/30023697 http://dx.doi.org/10.1021/acsomega.7b00353 |
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