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Functionalized Hydrophilic Superparamagnetic Iron Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in Liver Cancer Treatment
[Image: see text] In this work, we report the synthesis of hydrophilic and surface-functionalized superparamagnetic iron oxide nanoparticles (SPIOs) to utilize them as nanomedicines for treating liver cancer via magnetic fluid hyperthermia (MFH)-based thermotherapy. For this purpose, initially, we h...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044893/ https://www.ncbi.nlm.nih.gov/pubmed/30023884 http://dx.doi.org/10.1021/acsomega.8b00207 |
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author | Kandasamy, Ganeshlenin Sudame, Atul Luthra, Tania Saini, Kalawati Maity, Dipak |
author_facet | Kandasamy, Ganeshlenin Sudame, Atul Luthra, Tania Saini, Kalawati Maity, Dipak |
author_sort | Kandasamy, Ganeshlenin |
collection | PubMed |
description | [Image: see text] In this work, we report the synthesis of hydrophilic and surface-functionalized superparamagnetic iron oxide nanoparticles (SPIOs) to utilize them as nanomedicines for treating liver cancer via magnetic fluid hyperthermia (MFH)-based thermotherapy. For this purpose, initially, we have synthesized the SPIOs through co-precipitation/thermolysis methods, followed by in situ surface functionalization with short-chained molecules, such as 1,4-diaminobenzene (14DAB), 4-aminobenzoic acid (4ABA) and 3,4-diaminobenzoic acid (34DABA) and their combination with terephthalic acid (TA)/2-aminoterephthalic acid (ATA)/trimesic acid (TMA)/pyromellitic acid (PMA) molecules. The as-prepared SPIOs are investigated for their structure, morphology, water dispersibility, and magnetic properties. The heating efficacies of the SPIOs are studied in calorimetric MFH (C-MFH) with respect to their concentrations, surface coatings, dispersion medium, and applied alternating magnetic fields (AMFs). Although all of the as-prepared SPIOs have exhibited superparamagnetic behavior, only 14DAB-, 4ABA-, 34DABA-, and 4ABA-TA-coated SPIOs have shown higher magnetization values (M(s) = 55–71 emu g(–1)) and good water dispersibility. In C-MFH studies, 34DABA-coated SPIO-based aqueous ferrofluid (AFF) has revealed faster thermal response to the applied AMF and reached therapeutic temperature even at the lowest concentration (0.5 mg mL(–1)) compared with 14DAB-, 4ABA-, and 4ABA-TA-coated SPIO-based AFFs. Moreover, 34DABA-coated SPIO-based AFF has exhibited high heating efficacies (i.e., specific absorption rate/intrinsic loss power values of 432.1 W g(Fe)(–1)/5.2 nHm(2) kg(–1) at 0.5 mg mL(–1)), which could be mainly due to (i) enhanced π–π conjugation paths of surface-attached 34DABA coating molecules because of intrafunctional group attractions and (ii) improved anisotropy from the formation of clusters/linear chains of the SPIOs in ferrofluid suspensions, owing to interfunctional group attractions/interparticle interactions. Moreover, the 34DABA-coated SPIOs have demonstrated (i) very good cytocompatibility for 24/48 h incubation periods and (ii) higher killing efficiency of 61–88% (via MFH) in HepG2 liver cancer cells as compared to their treatment with only AMF/water-bath-based thermotherapy. In summary, the 34DABA-coated SPIOs are very promising heat-inducing agents for MFH-based thermotherapy and thus could be used as effective nanomedicines for cancer treatments. |
format | Online Article Text |
id | pubmed-6044893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-60448932018-07-16 Functionalized Hydrophilic Superparamagnetic Iron Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in Liver Cancer Treatment Kandasamy, Ganeshlenin Sudame, Atul Luthra, Tania Saini, Kalawati Maity, Dipak ACS Omega [Image: see text] In this work, we report the synthesis of hydrophilic and surface-functionalized superparamagnetic iron oxide nanoparticles (SPIOs) to utilize them as nanomedicines for treating liver cancer via magnetic fluid hyperthermia (MFH)-based thermotherapy. For this purpose, initially, we have synthesized the SPIOs through co-precipitation/thermolysis methods, followed by in situ surface functionalization with short-chained molecules, such as 1,4-diaminobenzene (14DAB), 4-aminobenzoic acid (4ABA) and 3,4-diaminobenzoic acid (34DABA) and their combination with terephthalic acid (TA)/2-aminoterephthalic acid (ATA)/trimesic acid (TMA)/pyromellitic acid (PMA) molecules. The as-prepared SPIOs are investigated for their structure, morphology, water dispersibility, and magnetic properties. The heating efficacies of the SPIOs are studied in calorimetric MFH (C-MFH) with respect to their concentrations, surface coatings, dispersion medium, and applied alternating magnetic fields (AMFs). Although all of the as-prepared SPIOs have exhibited superparamagnetic behavior, only 14DAB-, 4ABA-, 34DABA-, and 4ABA-TA-coated SPIOs have shown higher magnetization values (M(s) = 55–71 emu g(–1)) and good water dispersibility. In C-MFH studies, 34DABA-coated SPIO-based aqueous ferrofluid (AFF) has revealed faster thermal response to the applied AMF and reached therapeutic temperature even at the lowest concentration (0.5 mg mL(–1)) compared with 14DAB-, 4ABA-, and 4ABA-TA-coated SPIO-based AFFs. Moreover, 34DABA-coated SPIO-based AFF has exhibited high heating efficacies (i.e., specific absorption rate/intrinsic loss power values of 432.1 W g(Fe)(–1)/5.2 nHm(2) kg(–1) at 0.5 mg mL(–1)), which could be mainly due to (i) enhanced π–π conjugation paths of surface-attached 34DABA coating molecules because of intrafunctional group attractions and (ii) improved anisotropy from the formation of clusters/linear chains of the SPIOs in ferrofluid suspensions, owing to interfunctional group attractions/interparticle interactions. Moreover, the 34DABA-coated SPIOs have demonstrated (i) very good cytocompatibility for 24/48 h incubation periods and (ii) higher killing efficiency of 61–88% (via MFH) in HepG2 liver cancer cells as compared to their treatment with only AMF/water-bath-based thermotherapy. In summary, the 34DABA-coated SPIOs are very promising heat-inducing agents for MFH-based thermotherapy and thus could be used as effective nanomedicines for cancer treatments. American Chemical Society 2018-04-10 /pmc/articles/PMC6044893/ /pubmed/30023884 http://dx.doi.org/10.1021/acsomega.8b00207 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Kandasamy, Ganeshlenin Sudame, Atul Luthra, Tania Saini, Kalawati Maity, Dipak Functionalized Hydrophilic Superparamagnetic Iron Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in Liver Cancer Treatment |
title | Functionalized Hydrophilic
Superparamagnetic Iron
Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in
Liver Cancer Treatment |
title_full | Functionalized Hydrophilic
Superparamagnetic Iron
Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in
Liver Cancer Treatment |
title_fullStr | Functionalized Hydrophilic
Superparamagnetic Iron
Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in
Liver Cancer Treatment |
title_full_unstemmed | Functionalized Hydrophilic
Superparamagnetic Iron
Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in
Liver Cancer Treatment |
title_short | Functionalized Hydrophilic
Superparamagnetic Iron
Oxide Nanoparticles for Magnetic Fluid Hyperthermia Application in
Liver Cancer Treatment |
title_sort | functionalized hydrophilic
superparamagnetic iron
oxide nanoparticles for magnetic fluid hyperthermia application in
liver cancer treatment |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044893/ https://www.ncbi.nlm.nih.gov/pubmed/30023884 http://dx.doi.org/10.1021/acsomega.8b00207 |
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