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Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains

[Image: see text] A main challenge in understanding the structure of a cell membrane and its interactions with drugs is the ability to chemically study the different molecular species on the nanoscale. We have achieved this for a model system consisting of mixed monolayers (MLs) of the biologically...

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Autores principales: Kästner, Bernd, Johnson, C. Magnus, Hermann, Peter, Kruskopf, Mattias, Pierz, Klaus, Hoehl, Arne, Hornemann, Andrea, Ulrich, Georg, Fehmel, Jakob, Patoka, Piotr, Rühl, Eckart, Ulm, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044929/
https://www.ncbi.nlm.nih.gov/pubmed/30023886
http://dx.doi.org/10.1021/acsomega.7b01931
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author Kästner, Bernd
Johnson, C. Magnus
Hermann, Peter
Kruskopf, Mattias
Pierz, Klaus
Hoehl, Arne
Hornemann, Andrea
Ulrich, Georg
Fehmel, Jakob
Patoka, Piotr
Rühl, Eckart
Ulm, Gerhard
author_facet Kästner, Bernd
Johnson, C. Magnus
Hermann, Peter
Kruskopf, Mattias
Pierz, Klaus
Hoehl, Arne
Hornemann, Andrea
Ulrich, Georg
Fehmel, Jakob
Patoka, Piotr
Rühl, Eckart
Ulm, Gerhard
author_sort Kästner, Bernd
collection PubMed
description [Image: see text] A main challenge in understanding the structure of a cell membrane and its interactions with drugs is the ability to chemically study the different molecular species on the nanoscale. We have achieved this for a model system consisting of mixed monolayers (MLs) of the biologically relevant phospholipid 1,2-distearoyl-sn-glycero-phosphatidylcholine and the antibiotic surfactin. By employing nano-infrared (IR) microscopy and spectroscopy in combination with atomic force microscopy imaging, it was possible to identify and chemically detect domain formation of the two constituents as well as to obtain IR spectra of these species with a spatial resolution on the nanoscale. A novel method to enhance the near-field imaging contrast of organic MLs by plasmon interferometry is proposed and demonstrated. In this technique, the organic layer is deposited on gold and ML graphene substrates, the latter of which supports propagating surface plasmons. Plasmon reflections arising from changes in the dielectric environment provided by the organic layer lead to an additional contrast mechanism. Using this approach, the interfacial region between surfactin and the phospholipid has been mapped and a transition region is identified.
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spelling pubmed-60449292018-07-16 Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains Kästner, Bernd Johnson, C. Magnus Hermann, Peter Kruskopf, Mattias Pierz, Klaus Hoehl, Arne Hornemann, Andrea Ulrich, Georg Fehmel, Jakob Patoka, Piotr Rühl, Eckart Ulm, Gerhard ACS Omega [Image: see text] A main challenge in understanding the structure of a cell membrane and its interactions with drugs is the ability to chemically study the different molecular species on the nanoscale. We have achieved this for a model system consisting of mixed monolayers (MLs) of the biologically relevant phospholipid 1,2-distearoyl-sn-glycero-phosphatidylcholine and the antibiotic surfactin. By employing nano-infrared (IR) microscopy and spectroscopy in combination with atomic force microscopy imaging, it was possible to identify and chemically detect domain formation of the two constituents as well as to obtain IR spectra of these species with a spatial resolution on the nanoscale. A novel method to enhance the near-field imaging contrast of organic MLs by plasmon interferometry is proposed and demonstrated. In this technique, the organic layer is deposited on gold and ML graphene substrates, the latter of which supports propagating surface plasmons. Plasmon reflections arising from changes in the dielectric environment provided by the organic layer lead to an additional contrast mechanism. Using this approach, the interfacial region between surfactin and the phospholipid has been mapped and a transition region is identified. American Chemical Society 2018-04-12 /pmc/articles/PMC6044929/ /pubmed/30023886 http://dx.doi.org/10.1021/acsomega.7b01931 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kästner, Bernd
Johnson, C. Magnus
Hermann, Peter
Kruskopf, Mattias
Pierz, Klaus
Hoehl, Arne
Hornemann, Andrea
Ulrich, Georg
Fehmel, Jakob
Patoka, Piotr
Rühl, Eckart
Ulm, Gerhard
Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains
title Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains
title_full Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains
title_fullStr Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains
title_full_unstemmed Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains
title_short Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains
title_sort infrared nanospectroscopy of phospholipid and surfactin monolayer domains
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044929/
https://www.ncbi.nlm.nih.gov/pubmed/30023886
http://dx.doi.org/10.1021/acsomega.7b01931
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