In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria

[Image: see text] Herein, we introduce a new facile method of luminescent gold nanocluster (Au NC) synthesis on the surface of bacteria for detection, counting, and strain differentiation. The limit of detection was 740 ± 14 colony-forming unit (CFU)/mL for the Gram-negative and was 634 ± 16 CFU/mL...

Descripción completa

Detalles Bibliográficos
Autores principales: Goswami, Upashi, Sahoo, Amaresh Kumar, Chattopadhyay, Arun, Ghosh, Siddhartha Sankar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044939/
https://www.ncbi.nlm.nih.gov/pubmed/30023939
http://dx.doi.org/10.1021/acsomega.8b00504
_version_ 1783339577357893632
author Goswami, Upashi
Sahoo, Amaresh Kumar
Chattopadhyay, Arun
Ghosh, Siddhartha Sankar
author_facet Goswami, Upashi
Sahoo, Amaresh Kumar
Chattopadhyay, Arun
Ghosh, Siddhartha Sankar
author_sort Goswami, Upashi
collection PubMed
description [Image: see text] Herein, we introduce a new facile method of luminescent gold nanocluster (Au NC) synthesis on the surface of bacteria for detection, counting, and strain differentiation. The limit of detection was 740 ± 14 colony-forming unit (CFU)/mL for the Gram-negative and was 634 ± 16 CFU/mL for the Gram-positive bacteria. Brief treatment with lysozyme could differentiate the Gram strains based on their luminescence intensities. The current method could also detect bacterial contaminants from water sources and kanamycin-resistant strains rapidly. This quick synthesis of Au NCs on a bacterial template attributes an easy and rapid method for enumeration and detection of bacterial contaminants and kanamycin-resistant strains.
format Online
Article
Text
id pubmed-6044939
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-60449392018-07-16 In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria Goswami, Upashi Sahoo, Amaresh Kumar Chattopadhyay, Arun Ghosh, Siddhartha Sankar ACS Omega [Image: see text] Herein, we introduce a new facile method of luminescent gold nanocluster (Au NC) synthesis on the surface of bacteria for detection, counting, and strain differentiation. The limit of detection was 740 ± 14 colony-forming unit (CFU)/mL for the Gram-negative and was 634 ± 16 CFU/mL for the Gram-positive bacteria. Brief treatment with lysozyme could differentiate the Gram strains based on their luminescence intensities. The current method could also detect bacterial contaminants from water sources and kanamycin-resistant strains rapidly. This quick synthesis of Au NCs on a bacterial template attributes an easy and rapid method for enumeration and detection of bacterial contaminants and kanamycin-resistant strains. American Chemical Society 2018-06-06 /pmc/articles/PMC6044939/ /pubmed/30023939 http://dx.doi.org/10.1021/acsomega.8b00504 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Goswami, Upashi
Sahoo, Amaresh Kumar
Chattopadhyay, Arun
Ghosh, Siddhartha Sankar
In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria
title In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria
title_full In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria
title_fullStr In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria
title_full_unstemmed In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria
title_short In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria
title_sort in situ synthesis of luminescent au nanoclusters on a bacterial template for rapid detection, quantification, and distinction of kanamycin-resistant bacteria
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044939/
https://www.ncbi.nlm.nih.gov/pubmed/30023939
http://dx.doi.org/10.1021/acsomega.8b00504
work_keys_str_mv AT goswamiupashi insitusynthesisofluminescentaunanoclustersonabacterialtemplateforrapiddetectionquantificationanddistinctionofkanamycinresistantbacteria
AT sahooamareshkumar insitusynthesisofluminescentaunanoclustersonabacterialtemplateforrapiddetectionquantificationanddistinctionofkanamycinresistantbacteria
AT chattopadhyayarun insitusynthesisofluminescentaunanoclustersonabacterialtemplateforrapiddetectionquantificationanddistinctionofkanamycinresistantbacteria
AT ghoshsiddharthasankar insitusynthesisofluminescentaunanoclustersonabacterialtemplateforrapiddetectionquantificationanddistinctionofkanamycinresistantbacteria

Ejemplares similares