Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly

[Image: see text] In vitro mutagenesis methods have revolutionized biological research and the biotechnology industry. In this study, we describe a mutagenesis method based on synthesizing a gene using a complete set of forward and reverse spiked oligonucleotides that have been modified to introduce...

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Detalles Bibliográficos
Autores principales: Cárcamo, Edson, Roldán-Salgado, Abigail, Osuna, Joel, Bello-Sanmartin, Iván, Yáñez, Jorge A., Saab-Rincón, Gloria, Viadiu, Héctor, Gaytán, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044943/
https://www.ncbi.nlm.nih.gov/pubmed/30023688
http://dx.doi.org/10.1021/acsomega.7b00508

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