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Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water

[Image: see text] Globally, the need for “on-site” algal-toxin monitoring has become increasingly urgent due to the amplified demand for fresh-water and for safe, “toxin-free” shellfish and fish stocks. Herein, we describe the first reported, Lab-On-A-Disc (LOAD) based-platform developed to detect m...

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Autores principales: Maguire, Ivan, Fitzgerald, Jenny, Heery, Brendan, Nwankire, Charles, O’Kennedy, Richard, Ducrée, Jens, Regan, Fiona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045346/
https://www.ncbi.nlm.nih.gov/pubmed/30023955
http://dx.doi.org/10.1021/acsomega.8b00240
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author Maguire, Ivan
Fitzgerald, Jenny
Heery, Brendan
Nwankire, Charles
O’Kennedy, Richard
Ducrée, Jens
Regan, Fiona
author_facet Maguire, Ivan
Fitzgerald, Jenny
Heery, Brendan
Nwankire, Charles
O’Kennedy, Richard
Ducrée, Jens
Regan, Fiona
author_sort Maguire, Ivan
collection PubMed
description [Image: see text] Globally, the need for “on-site” algal-toxin monitoring has become increasingly urgent due to the amplified demand for fresh-water and for safe, “toxin-free” shellfish and fish stocks. Herein, we describe the first reported, Lab-On-A-Disc (LOAD) based-platform developed to detect microcystin levels in situ, with initial detectability of saxitoxin and domoic acid also reported. Using recombinant antibody technology, the LOAD platform combines immunofluorescence with centrifugally driven microfluidic liquid handling to achieve a next-generation disposable device capable of multianalyte sampling. A low-complexity “LED-photodiode” based optical sensing system was tailor-made for the platform, which allows the fluorescence signal of the toxin-specific reaction to be quantified. This system can rapidly and accurately detect the presence of microcystin-LR, domoic acid, and saxitoxin in 30 min, with a minimum of less than 5 min end-user interaction for maximum reproducibility. This method provides a robust “point of need” diagnostic alternative to the current laborious and costly methods used for qualitative toxin monitoring.
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spelling pubmed-60453462018-07-16 Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water Maguire, Ivan Fitzgerald, Jenny Heery, Brendan Nwankire, Charles O’Kennedy, Richard Ducrée, Jens Regan, Fiona ACS Omega [Image: see text] Globally, the need for “on-site” algal-toxin monitoring has become increasingly urgent due to the amplified demand for fresh-water and for safe, “toxin-free” shellfish and fish stocks. Herein, we describe the first reported, Lab-On-A-Disc (LOAD) based-platform developed to detect microcystin levels in situ, with initial detectability of saxitoxin and domoic acid also reported. Using recombinant antibody technology, the LOAD platform combines immunofluorescence with centrifugally driven microfluidic liquid handling to achieve a next-generation disposable device capable of multianalyte sampling. A low-complexity “LED-photodiode” based optical sensing system was tailor-made for the platform, which allows the fluorescence signal of the toxin-specific reaction to be quantified. This system can rapidly and accurately detect the presence of microcystin-LR, domoic acid, and saxitoxin in 30 min, with a minimum of less than 5 min end-user interaction for maximum reproducibility. This method provides a robust “point of need” diagnostic alternative to the current laborious and costly methods used for qualitative toxin monitoring. American Chemical Society 2018-06-20 /pmc/articles/PMC6045346/ /pubmed/30023955 http://dx.doi.org/10.1021/acsomega.8b00240 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Maguire, Ivan
Fitzgerald, Jenny
Heery, Brendan
Nwankire, Charles
O’Kennedy, Richard
Ducrée, Jens
Regan, Fiona
Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
title Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
title_full Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
title_fullStr Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
title_full_unstemmed Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
title_short Novel Microfluidic Analytical Sensing Platform for the Simultaneous Detection of Three Algal Toxins in Water
title_sort novel microfluidic analytical sensing platform for the simultaneous detection of three algal toxins in water
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045346/
https://www.ncbi.nlm.nih.gov/pubmed/30023955
http://dx.doi.org/10.1021/acsomega.8b00240
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