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Streamlined Preparation of Immobilized Candida antarctica Lipase B
[Image: see text] Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L(–1)) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catal...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045393/ https://www.ncbi.nlm.nih.gov/pubmed/30023589 http://dx.doi.org/10.1021/acsomega.7b01510 |
| _version_ | 1783339659815813120 |
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| author | Cassimjee, Karim Engelmark Hendil-Forssell, Peter Volkov, Alexey Krog, Anne Malmo, Jostein Aune, Trond Erik V. Knecht, Wolfgang Miskelly, Iain R. Moody, Thomas S. Svedendahl Humble, Maria |
| author_facet | Cassimjee, Karim Engelmark Hendil-Forssell, Peter Volkov, Alexey Krog, Anne Malmo, Jostein Aune, Trond Erik V. Knecht, Wolfgang Miskelly, Iain R. Moody, Thomas S. Svedendahl Humble, Maria |
| author_sort | Cassimjee, Karim Engelmark |
| collection | PubMed |
| description | [Image: see text] Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L(–1)) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435. |
| format | Online Article Text |
| id | pubmed-6045393 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| publisher | American Chemical Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-60453932018-07-16 Streamlined Preparation of Immobilized Candida antarctica Lipase B Cassimjee, Karim Engelmark Hendil-Forssell, Peter Volkov, Alexey Krog, Anne Malmo, Jostein Aune, Trond Erik V. Knecht, Wolfgang Miskelly, Iain R. Moody, Thomas S. Svedendahl Humble, Maria ACS Omega [Image: see text] Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L(–1)) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435. American Chemical Society 2017-12-06 /pmc/articles/PMC6045393/ /pubmed/30023589 http://dx.doi.org/10.1021/acsomega.7b01510 Text en Copyright © 2017 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
| spellingShingle | Cassimjee, Karim Engelmark Hendil-Forssell, Peter Volkov, Alexey Krog, Anne Malmo, Jostein Aune, Trond Erik V. Knecht, Wolfgang Miskelly, Iain R. Moody, Thomas S. Svedendahl Humble, Maria Streamlined Preparation of Immobilized Candida antarctica Lipase B |
| title | Streamlined Preparation of Immobilized Candida antarctica Lipase B |
| title_full | Streamlined Preparation of Immobilized Candida antarctica Lipase B |
| title_fullStr | Streamlined Preparation of Immobilized Candida antarctica Lipase B |
| title_full_unstemmed | Streamlined Preparation of Immobilized Candida antarctica Lipase B |
| title_short | Streamlined Preparation of Immobilized Candida antarctica Lipase B |
| title_sort | streamlined preparation of immobilized candida antarctica lipase b |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045393/ https://www.ncbi.nlm.nih.gov/pubmed/30023589 http://dx.doi.org/10.1021/acsomega.7b01510 |
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