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Streamlined Preparation of Immobilized Candida antarctica Lipase B
[Image: see text] Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L(–1)) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catal...
Autores principales: | Cassimjee, Karim Engelmark, Hendil-Forssell, Peter, Volkov, Alexey, Krog, Anne, Malmo, Jostein, Aune, Trond Erik V., Knecht, Wolfgang, Miskelly, Iain R., Moody, Thomas S., Svedendahl Humble, Maria |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045393/ https://www.ncbi.nlm.nih.gov/pubmed/30023589 http://dx.doi.org/10.1021/acsomega.7b01510 |
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