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Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology

[Image: see text] Owing to the dwindling arsenal of antibiotics, new methodologies for their effective and localized delivery are necessary. The use of optical control over delivery of drugs, also known as photopharmacology, has emerged as an important option for the spatiotemporally controlled gene...

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Autores principales: Kumari, Pooja, Kulkarni, Amogh, Sharma, Ajay Kumar, Chakrapani, Harinath
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045474/
https://www.ncbi.nlm.nih.gov/pubmed/30023825
http://dx.doi.org/10.1021/acsomega.7b01906
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author Kumari, Pooja
Kulkarni, Amogh
Sharma, Ajay Kumar
Chakrapani, Harinath
author_facet Kumari, Pooja
Kulkarni, Amogh
Sharma, Ajay Kumar
Chakrapani, Harinath
author_sort Kumari, Pooja
collection PubMed
description [Image: see text] Owing to the dwindling arsenal of antibiotics, new methodologies for their effective and localized delivery are necessary. The use of optical control over delivery of drugs, also known as photopharmacology, has emerged as an important option for the spatiotemporally controlled generation of drugs and bioactive molecules. In the field of antimicrobial photopharmacology, most strategies utilize ultraviolet light for triggering release of the antibiotic. The use of such short wavelength light may have limitations such as phototoxicity. Here, a small molecule that is activated by visible light to release a fluoroquinolone, a broad-spectrum antibiotic, is reported. A boron-dipyrromethene, which is sensitive to cleavage at 470 nm, was used, and levofloxacin was used as a model fluoroquinolone. BDP-Levo was found to undergo cleavage in the presence of visible light to release the active antibiotic. Using growth inhibitory studies in Gram-positive as well as Gram-negative bacteria, the efficacy of BDP-Levo is demonstrated. Together, our study demonstrates that visible light can be used for optical control over antibiotic release and lays the foundation for visible-light-mediated antimicrobial photopharmacology.
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spelling pubmed-60454742018-07-16 Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology Kumari, Pooja Kulkarni, Amogh Sharma, Ajay Kumar Chakrapani, Harinath ACS Omega [Image: see text] Owing to the dwindling arsenal of antibiotics, new methodologies for their effective and localized delivery are necessary. The use of optical control over delivery of drugs, also known as photopharmacology, has emerged as an important option for the spatiotemporally controlled generation of drugs and bioactive molecules. In the field of antimicrobial photopharmacology, most strategies utilize ultraviolet light for triggering release of the antibiotic. The use of such short wavelength light may have limitations such as phototoxicity. Here, a small molecule that is activated by visible light to release a fluoroquinolone, a broad-spectrum antibiotic, is reported. A boron-dipyrromethene, which is sensitive to cleavage at 470 nm, was used, and levofloxacin was used as a model fluoroquinolone. BDP-Levo was found to undergo cleavage in the presence of visible light to release the active antibiotic. Using growth inhibitory studies in Gram-positive as well as Gram-negative bacteria, the efficacy of BDP-Levo is demonstrated. Together, our study demonstrates that visible light can be used for optical control over antibiotic release and lays the foundation for visible-light-mediated antimicrobial photopharmacology. American Chemical Society 2018-02-21 /pmc/articles/PMC6045474/ /pubmed/30023825 http://dx.doi.org/10.1021/acsomega.7b01906 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Kumari, Pooja
Kulkarni, Amogh
Sharma, Ajay Kumar
Chakrapani, Harinath
Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology
title Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology
title_full Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology
title_fullStr Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology
title_full_unstemmed Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology
title_short Visible-Light Controlled Release of a Fluoroquinolone Antibiotic for Antimicrobial Photopharmacology
title_sort visible-light controlled release of a fluoroquinolone antibiotic for antimicrobial photopharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045474/
https://www.ncbi.nlm.nih.gov/pubmed/30023825
http://dx.doi.org/10.1021/acsomega.7b01906
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