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Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice
Environmental air pollutants including ozone cause severe lung injury and aggravate respiratory diseases such as asthma and COPD. Here we compared the effect of ozone on respiratory epithelium injury, inflammation, hyperreactivity and airway remodeling in mice upon acute (1ppm, 1 h) and chronic expo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045627/ https://www.ncbi.nlm.nih.gov/pubmed/30006538 http://dx.doi.org/10.1038/s41598-018-28261-9 |
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author | Michaudel, Chloé Fauconnier, Louis Julé, Yvon Ryffel, Bernhard |
author_facet | Michaudel, Chloé Fauconnier, Louis Julé, Yvon Ryffel, Bernhard |
author_sort | Michaudel, Chloé |
collection | PubMed |
description | Environmental air pollutants including ozone cause severe lung injury and aggravate respiratory diseases such as asthma and COPD. Here we compared the effect of ozone on respiratory epithelium injury, inflammation, hyperreactivity and airway remodeling in mice upon acute (1ppm, 1 h) and chronic exposure (1.5ppm, 2 h, twice weekly for 6 weeks). Acute ozone exposure caused respiratory epithelial disruption with protein leak and neutrophil recruitment in the broncho-alveolar space, leading to lung inflammation and airway hyperresponsiveness (AHR) to methacholine. All these parameters were increased upon chronic ozone exposure, including collagen deposition. The structure of the airways as assessed by automatic numerical image analysis showed significant differences: While acute ozone exposure increased bronchial and lumen circularity but decreased epithelial thickness and area, chronic ozone exposure revealed epithelial injury with reduced height, distended bronchioles, enlarged alveolar space and increased collagen deposition, indicative of peribronchiolar fibrosis and emphysema as characterized by a significant increase in the density and diameter of airspaces with decreased airspace numbers. In conclusion, morphometric numerical analysis enables an automatic and unbiased assessment of small airway remodeling. The structural changes of the small airways correlated with functional changes allowing to follow the progression from acute to chronic ozone induced respiratory pathology. |
format | Online Article Text |
id | pubmed-6045627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60456272018-07-16 Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice Michaudel, Chloé Fauconnier, Louis Julé, Yvon Ryffel, Bernhard Sci Rep Article Environmental air pollutants including ozone cause severe lung injury and aggravate respiratory diseases such as asthma and COPD. Here we compared the effect of ozone on respiratory epithelium injury, inflammation, hyperreactivity and airway remodeling in mice upon acute (1ppm, 1 h) and chronic exposure (1.5ppm, 2 h, twice weekly for 6 weeks). Acute ozone exposure caused respiratory epithelial disruption with protein leak and neutrophil recruitment in the broncho-alveolar space, leading to lung inflammation and airway hyperresponsiveness (AHR) to methacholine. All these parameters were increased upon chronic ozone exposure, including collagen deposition. The structure of the airways as assessed by automatic numerical image analysis showed significant differences: While acute ozone exposure increased bronchial and lumen circularity but decreased epithelial thickness and area, chronic ozone exposure revealed epithelial injury with reduced height, distended bronchioles, enlarged alveolar space and increased collagen deposition, indicative of peribronchiolar fibrosis and emphysema as characterized by a significant increase in the density and diameter of airspaces with decreased airspace numbers. In conclusion, morphometric numerical analysis enables an automatic and unbiased assessment of small airway remodeling. The structural changes of the small airways correlated with functional changes allowing to follow the progression from acute to chronic ozone induced respiratory pathology. Nature Publishing Group UK 2018-07-13 /pmc/articles/PMC6045627/ /pubmed/30006538 http://dx.doi.org/10.1038/s41598-018-28261-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Michaudel, Chloé Fauconnier, Louis Julé, Yvon Ryffel, Bernhard Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
title | Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
title_full | Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
title_fullStr | Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
title_full_unstemmed | Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
title_short | Functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
title_sort | functional and morphological differences of the lung upon acute and chronic ozone exposure in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045627/ https://www.ncbi.nlm.nih.gov/pubmed/30006538 http://dx.doi.org/10.1038/s41598-018-28261-9 |
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