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Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone

The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for bioana...

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Autores principales: Temiz, Yuksel, Delamarche, Emmanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045673/
https://www.ncbi.nlm.nih.gov/pubmed/30006576
http://dx.doi.org/10.1038/s41598-018-28983-w
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author Temiz, Yuksel
Delamarche, Emmanuel
author_facet Temiz, Yuksel
Delamarche, Emmanuel
author_sort Temiz, Yuksel
collection PubMed
description The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for bioanalysis. However, filling microfluidic devices with liquid can be prone to failure. For this reason, we have implemented a simple, yet efficient method for monitoring liquid displacement in microfluidic chips using capacitive sensing and a compact (75 mm × 30 mm × 10 mm), low-cost ($60), and battery-powered (10-hour autonomy) device communicating with a smartphone. We demonstrated the concept using a capillary-driven microfluidic chip comprising two equivalent flow paths, each with a total volume of 420 nL. Capacitance measurements from a pair of electrodes patterned longitudinally along the flow paths yielded 17 pL resolution in monitoring liquid displacement at a sampling rate of 1 data/s (~1 nL/min resolution in the flow rate). We characterized the system using human serum, biological buffers, and water, and implemented an algorithm to provide real-time information on flow conditions occurring in a microfluidic chip and interactive guidance to the user.
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spelling pubmed-60456732018-07-16 Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone Temiz, Yuksel Delamarche, Emmanuel Sci Rep Article The ever-increasing need for portable, easy-to-use, cost-effective, and connected point-of-care diagnostics (POCD) has been one of the main drivers of recent research on lab-on-a-chip (LoC) devices. A majority of these devices use microfluidics to manipulate precisely samples and reagents for bioanalysis. However, filling microfluidic devices with liquid can be prone to failure. For this reason, we have implemented a simple, yet efficient method for monitoring liquid displacement in microfluidic chips using capacitive sensing and a compact (75 mm × 30 mm × 10 mm), low-cost ($60), and battery-powered (10-hour autonomy) device communicating with a smartphone. We demonstrated the concept using a capillary-driven microfluidic chip comprising two equivalent flow paths, each with a total volume of 420 nL. Capacitance measurements from a pair of electrodes patterned longitudinally along the flow paths yielded 17 pL resolution in monitoring liquid displacement at a sampling rate of 1 data/s (~1 nL/min resolution in the flow rate). We characterized the system using human serum, biological buffers, and water, and implemented an algorithm to provide real-time information on flow conditions occurring in a microfluidic chip and interactive guidance to the user. Nature Publishing Group UK 2018-07-13 /pmc/articles/PMC6045673/ /pubmed/30006576 http://dx.doi.org/10.1038/s41598-018-28983-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Temiz, Yuksel
Delamarche, Emmanuel
Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
title Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
title_full Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
title_fullStr Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
title_full_unstemmed Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
title_short Sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
title_sort sub-nanoliter, real-time flow monitoring in microfluidic chips using a portable device and smartphone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045673/
https://www.ncbi.nlm.nih.gov/pubmed/30006576
http://dx.doi.org/10.1038/s41598-018-28983-w
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