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Rehydration with fructose worsens dehydration-induced renal damage

BACKGROUND: Increasing evidence suggests heat stress induced chronic kidney disease (CKD) may be mediated by endogenous fructose generation and may be exacerbated by rehydration by fructose-containing solutions. We have recently reported a model of CKD induced by heat stress. Here we test the hypoth...

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Autores principales: Milagres, Tamara, García-Arroyo, Fernando E., Lanaspa, Miguel A., Garcia, Gabriela, Ishimoto, Takuji, Andres-Hernando, Ana, Kuwabara, Masanari, Jensen, Thomas, Sato, Yuka, Glaser, Jason, Sánchez-Lozada, Laura G., Johnson, Richard J., Roncal-Jimenez, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045876/
https://www.ncbi.nlm.nih.gov/pubmed/30005632
http://dx.doi.org/10.1186/s12882-018-0963-9
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author Milagres, Tamara
García-Arroyo, Fernando E.
Lanaspa, Miguel A.
Garcia, Gabriela
Ishimoto, Takuji
Andres-Hernando, Ana
Kuwabara, Masanari
Jensen, Thomas
Sato, Yuka
Glaser, Jason
Sánchez-Lozada, Laura G.
Johnson, Richard J.
Roncal-Jimenez, Carlos
author_facet Milagres, Tamara
García-Arroyo, Fernando E.
Lanaspa, Miguel A.
Garcia, Gabriela
Ishimoto, Takuji
Andres-Hernando, Ana
Kuwabara, Masanari
Jensen, Thomas
Sato, Yuka
Glaser, Jason
Sánchez-Lozada, Laura G.
Johnson, Richard J.
Roncal-Jimenez, Carlos
author_sort Milagres, Tamara
collection PubMed
description BACKGROUND: Increasing evidence suggests heat stress induced chronic kidney disease (CKD) may be mediated by endogenous fructose generation and may be exacerbated by rehydration by fructose-containing solutions. We have recently reported a model of CKD induced by heat stress. Here we test the hypothesis that rehydration with fructose may induce worse kidney injury than rehydration with equal amounts of water, and we also test if this fructose-induced injury is associated with activation of inflammasomes in the kidney. METHODS: Mice were recurrently exposed to heat (39.5 C(0) for 30 min/h, 5 times daily for 5 wks) with rehydration consisting of 6 ml each night of water (Heat, n = 7) or fructose (Heat+F, 10%, n = 7), and were compared to control mice on water (Control, n = 7) or fructose (Fructose, n = 7). Various markers of renal injury were assessed. RESULTS: Compared to control animals, there was a progressive worsening of renal injury (inflammation and fibrosis) with fructose alone, heat stress alone, and heat stress with fructose rehydration (P < 0.01 by ANOVA). The combination of heat stress with rehydration with fructose was associated with increased intrarenal expression of the inflammasome markers, NLRP3 and IL-18, compared to heat stress alone. In addition, heat stress with or without fructose was associated with increased expression of caspase − 3 and monocyte chemoattractant protein-1 levels. Fructose administration was also associated with an increase in serum copeptin levels (a biomarker of vasopressin) and elevated copeptin was also observed in mice undergoing heat stress alone. CONCLUSIONS: These studies suggest that heat stress may activate intrarenal inflammasomes leading to inflammation and renal injury, and provide evidence that rehydration with fructose may accelerate the renal injury and inflammatory response.
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spelling pubmed-60458762018-07-16 Rehydration with fructose worsens dehydration-induced renal damage Milagres, Tamara García-Arroyo, Fernando E. Lanaspa, Miguel A. Garcia, Gabriela Ishimoto, Takuji Andres-Hernando, Ana Kuwabara, Masanari Jensen, Thomas Sato, Yuka Glaser, Jason Sánchez-Lozada, Laura G. Johnson, Richard J. Roncal-Jimenez, Carlos BMC Nephrol Research Article BACKGROUND: Increasing evidence suggests heat stress induced chronic kidney disease (CKD) may be mediated by endogenous fructose generation and may be exacerbated by rehydration by fructose-containing solutions. We have recently reported a model of CKD induced by heat stress. Here we test the hypothesis that rehydration with fructose may induce worse kidney injury than rehydration with equal amounts of water, and we also test if this fructose-induced injury is associated with activation of inflammasomes in the kidney. METHODS: Mice were recurrently exposed to heat (39.5 C(0) for 30 min/h, 5 times daily for 5 wks) with rehydration consisting of 6 ml each night of water (Heat, n = 7) or fructose (Heat+F, 10%, n = 7), and were compared to control mice on water (Control, n = 7) or fructose (Fructose, n = 7). Various markers of renal injury were assessed. RESULTS: Compared to control animals, there was a progressive worsening of renal injury (inflammation and fibrosis) with fructose alone, heat stress alone, and heat stress with fructose rehydration (P < 0.01 by ANOVA). The combination of heat stress with rehydration with fructose was associated with increased intrarenal expression of the inflammasome markers, NLRP3 and IL-18, compared to heat stress alone. In addition, heat stress with or without fructose was associated with increased expression of caspase − 3 and monocyte chemoattractant protein-1 levels. Fructose administration was also associated with an increase in serum copeptin levels (a biomarker of vasopressin) and elevated copeptin was also observed in mice undergoing heat stress alone. CONCLUSIONS: These studies suggest that heat stress may activate intrarenal inflammasomes leading to inflammation and renal injury, and provide evidence that rehydration with fructose may accelerate the renal injury and inflammatory response. BioMed Central 2018-07-13 /pmc/articles/PMC6045876/ /pubmed/30005632 http://dx.doi.org/10.1186/s12882-018-0963-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Milagres, Tamara
García-Arroyo, Fernando E.
Lanaspa, Miguel A.
Garcia, Gabriela
Ishimoto, Takuji
Andres-Hernando, Ana
Kuwabara, Masanari
Jensen, Thomas
Sato, Yuka
Glaser, Jason
Sánchez-Lozada, Laura G.
Johnson, Richard J.
Roncal-Jimenez, Carlos
Rehydration with fructose worsens dehydration-induced renal damage
title Rehydration with fructose worsens dehydration-induced renal damage
title_full Rehydration with fructose worsens dehydration-induced renal damage
title_fullStr Rehydration with fructose worsens dehydration-induced renal damage
title_full_unstemmed Rehydration with fructose worsens dehydration-induced renal damage
title_short Rehydration with fructose worsens dehydration-induced renal damage
title_sort rehydration with fructose worsens dehydration-induced renal damage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045876/
https://www.ncbi.nlm.nih.gov/pubmed/30005632
http://dx.doi.org/10.1186/s12882-018-0963-9
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