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Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis

BACKGROUND: The traditional Chinese formula Da-Cheng-Qi-decoction (DCQD) has been used to treat acute pancreatitis for decades. DCQD could ameliorate the disease severity and the complications of organ injuries, including those of the liver and lungs. However, the pharmacological effects in the kidn...

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Autores principales: Yuan, Ling, Zhu, Lv, Zhang, Yumei, Chen, Huan, Kang, Hongxin, Li, Juan, Zhao, Xianlin, Wan, Meihua, Miao, Yifan, Tang, Wenfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045888/
https://www.ncbi.nlm.nih.gov/pubmed/30013616
http://dx.doi.org/10.1186/s13020-018-0195-8
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author Yuan, Ling
Zhu, Lv
Zhang, Yumei
Chen, Huan
Kang, Hongxin
Li, Juan
Zhao, Xianlin
Wan, Meihua
Miao, Yifan
Tang, Wenfu
author_facet Yuan, Ling
Zhu, Lv
Zhang, Yumei
Chen, Huan
Kang, Hongxin
Li, Juan
Zhao, Xianlin
Wan, Meihua
Miao, Yifan
Tang, Wenfu
author_sort Yuan, Ling
collection PubMed
description BACKGROUND: The traditional Chinese formula Da-Cheng-Qi-decoction (DCQD) has been used to treat acute pancreatitis for decades. DCQD could ameliorate the disease severity and the complications of organ injuries, including those of the liver and lungs. However, the pharmacological effects in the kidney, a target organ, are still unclear. This study aimed to investigate the herbal tissue pharmacology of DCQD for acute kidney injury (AKI) in rats with severe acute pancreatitis (SAP). METHODS: Rats were randomly divided into the sham-operation group (SG), the model group (MG) and the low-, medium- and high-dose treatment groups (LDG, MDG, and HDG, respectively). Sodium taurocholate (3.5%) was retrogradely perfused into the biliopancreatic duct to establish the model of SAP in rats. Different doses of DCQD were administered to the treatment groups 2 h after the induction of SAP. The major components of DCQD in kidney tissues were detected by HPLC–MS/MS. Inflammatory mediators in the kidney tissues, as well as serum creatinine (Scr), blood urea nitrogen (BUN) and pathologic scores, were also evaluated. RESULTS: Ten components of DCQD were detected in the kidneys of the treatment groups, and their concentrations increased dose-dependently. Compared with the SG, the levels of inflammatory mediators, Scr, BUN and pathological scores in the MG were obviously increased (p < 0.05). The high dose of DCQD showed a maximal effect in downregulating the pro-inflammatory mediators interleukin-6 (IL)-6 and tumour necrosis factor-α (TNF-α), upregulating anti-inflammatory mediators IL-4 and IL-10 in the kidney and alleviating the pathological damages. DCQD decreased the pancreas and kidney pathological scores of rats with SAP, especially in the HDG (p < 0.05). Compared with the MG, the level of Scr in the HDG was significantly decreased (p < 0.05). CONCLUSIONS: DCQD ameliorated AKI in rats with SAP via regulating the inflammatory response, which might be closely related to the distribution of its components in the kidney. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0195-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-60458882018-07-16 Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis Yuan, Ling Zhu, Lv Zhang, Yumei Chen, Huan Kang, Hongxin Li, Juan Zhao, Xianlin Wan, Meihua Miao, Yifan Tang, Wenfu Chin Med Research BACKGROUND: The traditional Chinese formula Da-Cheng-Qi-decoction (DCQD) has been used to treat acute pancreatitis for decades. DCQD could ameliorate the disease severity and the complications of organ injuries, including those of the liver and lungs. However, the pharmacological effects in the kidney, a target organ, are still unclear. This study aimed to investigate the herbal tissue pharmacology of DCQD for acute kidney injury (AKI) in rats with severe acute pancreatitis (SAP). METHODS: Rats were randomly divided into the sham-operation group (SG), the model group (MG) and the low-, medium- and high-dose treatment groups (LDG, MDG, and HDG, respectively). Sodium taurocholate (3.5%) was retrogradely perfused into the biliopancreatic duct to establish the model of SAP in rats. Different doses of DCQD were administered to the treatment groups 2 h after the induction of SAP. The major components of DCQD in kidney tissues were detected by HPLC–MS/MS. Inflammatory mediators in the kidney tissues, as well as serum creatinine (Scr), blood urea nitrogen (BUN) and pathologic scores, were also evaluated. RESULTS: Ten components of DCQD were detected in the kidneys of the treatment groups, and their concentrations increased dose-dependently. Compared with the SG, the levels of inflammatory mediators, Scr, BUN and pathological scores in the MG were obviously increased (p < 0.05). The high dose of DCQD showed a maximal effect in downregulating the pro-inflammatory mediators interleukin-6 (IL)-6 and tumour necrosis factor-α (TNF-α), upregulating anti-inflammatory mediators IL-4 and IL-10 in the kidney and alleviating the pathological damages. DCQD decreased the pancreas and kidney pathological scores of rats with SAP, especially in the HDG (p < 0.05). Compared with the MG, the level of Scr in the HDG was significantly decreased (p < 0.05). CONCLUSIONS: DCQD ameliorated AKI in rats with SAP via regulating the inflammatory response, which might be closely related to the distribution of its components in the kidney. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13020-018-0195-8) contains supplementary material, which is available to authorized users. BioMed Central 2018-07-13 /pmc/articles/PMC6045888/ /pubmed/30013616 http://dx.doi.org/10.1186/s13020-018-0195-8 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yuan, Ling
Zhu, Lv
Zhang, Yumei
Chen, Huan
Kang, Hongxin
Li, Juan
Zhao, Xianlin
Wan, Meihua
Miao, Yifan
Tang, Wenfu
Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
title Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
title_full Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
title_fullStr Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
title_full_unstemmed Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
title_short Effect of Da-Cheng-Qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
title_sort effect of da-cheng-qi decoction for treatment of acute kidney injury in rats with severe acute pancreatitis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045888/
https://www.ncbi.nlm.nih.gov/pubmed/30013616
http://dx.doi.org/10.1186/s13020-018-0195-8
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