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Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom

BACKGROUND: We investigated the hemodynamic changes (Inotropic, chronotropic and arrhythmogenic) in intravenously envenomed anesthetized rats with Hemiscorpius lepturus venom. The neutralizing potencies of different drugs and commercial antivenom were assessed simultaneously. METHODS: Different dose...

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Autores principales: Pourkhalili, Khalil, Fatemikia, Hossein, Kim, Euikyung, Mashayekhy, Navid Reza, Dounighi, Naser Mohammadpour, Hajivandi, Abdollah, Hassan, Yaghoob, Seyedian, Ramin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046103/
https://www.ncbi.nlm.nih.gov/pubmed/30018992
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author Pourkhalili, Khalil
Fatemikia, Hossein
Kim, Euikyung
Mashayekhy, Navid Reza
Dounighi, Naser Mohammadpour
Hajivandi, Abdollah
Hassan, Yaghoob
Seyedian, Ramin
author_facet Pourkhalili, Khalil
Fatemikia, Hossein
Kim, Euikyung
Mashayekhy, Navid Reza
Dounighi, Naser Mohammadpour
Hajivandi, Abdollah
Hassan, Yaghoob
Seyedian, Ramin
author_sort Pourkhalili, Khalil
collection PubMed
description BACKGROUND: We investigated the hemodynamic changes (Inotropic, chronotropic and arrhythmogenic) in intravenously envenomed anesthetized rats with Hemiscorpius lepturus venom. The neutralizing potencies of different drugs and commercial antivenom were assessed simultaneously. METHODS: Different doses of the crude venom (100, 200 and 400μg/rat) were injected during five minutes via the femoral vein and cardiovascular changes were recorded in rats in Razi Institute Corporation, Karaj, Iran in 2017. The drugs (Atropine, lidocaine, propranolol and prazosin) were injected before the venom for determination of the counteracting effects. Different volumes (100, 500 and 1000μl) of the antivenom were pre envenomed to neutralize cardiovascular changes. RESULTS: Temporary hypertension and bradycardia with no arrhythmogenic effects were depicted within twenty minutes. There was a difference in arterial pressure between the venom (400μg/rat) and the vehicle at 8 minutes (114.68±5.1mmHg versus 70.2±4.3mmHg). Elevation of the mean arterial pressure was inhibited by propranolol (2 mg/kg) and neutralized by prazosin (1mg/kg) while lidocaine (4mg/kg) and atropine (1mg/kg) had no effects. Premedication with Iranian commercial antivenom (1000μl) produced surprisingly temporary hypertension compared to the vehicle (140.84±4.5 versus 84.3±3.2). It had no neutralizing properties on blood pressure variation before the venom injection. Volume-expanded hypertension phenomenon was ruled out in a parallel study. CONCLUSION: This venom has vasoconstrictive effects in rats probably due to the presence of norepinephrine like materials in its content or liberated from adrenal gland inhibited by prazosin premedication. The neutralizing effects of antivenom on venom-induced hypertension are questionable.
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spelling pubmed-60461032018-07-17 Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom Pourkhalili, Khalil Fatemikia, Hossein Kim, Euikyung Mashayekhy, Navid Reza Dounighi, Naser Mohammadpour Hajivandi, Abdollah Hassan, Yaghoob Seyedian, Ramin J Arthropod Borne Dis Original Article BACKGROUND: We investigated the hemodynamic changes (Inotropic, chronotropic and arrhythmogenic) in intravenously envenomed anesthetized rats with Hemiscorpius lepturus venom. The neutralizing potencies of different drugs and commercial antivenom were assessed simultaneously. METHODS: Different doses of the crude venom (100, 200 and 400μg/rat) were injected during five minutes via the femoral vein and cardiovascular changes were recorded in rats in Razi Institute Corporation, Karaj, Iran in 2017. The drugs (Atropine, lidocaine, propranolol and prazosin) were injected before the venom for determination of the counteracting effects. Different volumes (100, 500 and 1000μl) of the antivenom were pre envenomed to neutralize cardiovascular changes. RESULTS: Temporary hypertension and bradycardia with no arrhythmogenic effects were depicted within twenty minutes. There was a difference in arterial pressure between the venom (400μg/rat) and the vehicle at 8 minutes (114.68±5.1mmHg versus 70.2±4.3mmHg). Elevation of the mean arterial pressure was inhibited by propranolol (2 mg/kg) and neutralized by prazosin (1mg/kg) while lidocaine (4mg/kg) and atropine (1mg/kg) had no effects. Premedication with Iranian commercial antivenom (1000μl) produced surprisingly temporary hypertension compared to the vehicle (140.84±4.5 versus 84.3±3.2). It had no neutralizing properties on blood pressure variation before the venom injection. Volume-expanded hypertension phenomenon was ruled out in a parallel study. CONCLUSION: This venom has vasoconstrictive effects in rats probably due to the presence of norepinephrine like materials in its content or liberated from adrenal gland inhibited by prazosin premedication. The neutralizing effects of antivenom on venom-induced hypertension are questionable. Tehran University of Medical Sciences 2018-03-18 /pmc/articles/PMC6046103/ /pubmed/30018992 Text en Copyright© Iranian Society of Medical Entomology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Pourkhalili, Khalil
Fatemikia, Hossein
Kim, Euikyung
Mashayekhy, Navid Reza
Dounighi, Naser Mohammadpour
Hajivandi, Abdollah
Hassan, Yaghoob
Seyedian, Ramin
Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom
title Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom
title_full Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom
title_fullStr Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom
title_full_unstemmed Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom
title_short Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom
title_sort hemodynamic changes in experimentally envenomed anaesthetized rats by intravenous injection of hemiscorpius lepturus venom
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046103/
https://www.ncbi.nlm.nih.gov/pubmed/30018992
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