Cargando…

Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice

Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (S...

Descripción completa

Detalles Bibliográficos
Autores principales: Seldeen, Kenneth L., Pang, Manhui, Leiker, Merced M., Bard, Jonathan E., Rodríguez-Gonzalez, Maria, Hernandez, Mireya, Sheridan, Zachary, Nowak, Norma, Troen, Bruce R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046224/
https://www.ncbi.nlm.nih.gov/pubmed/29905532
http://dx.doi.org/10.18632/aging.101471
_version_ 1783339791932194816
author Seldeen, Kenneth L.
Pang, Manhui
Leiker, Merced M.
Bard, Jonathan E.
Rodríguez-Gonzalez, Maria
Hernandez, Mireya
Sheridan, Zachary
Nowak, Norma
Troen, Bruce R.
author_facet Seldeen, Kenneth L.
Pang, Manhui
Leiker, Merced M.
Bard, Jonathan E.
Rodríguez-Gonzalez, Maria
Hernandez, Mireya
Sheridan, Zachary
Nowak, Norma
Troen, Bruce R.
author_sort Seldeen, Kenneth L.
collection PubMed
description Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (STD, 1000 IU) or insufficient (LOW, 125 IU) vitamin D3/kg chow for 12 months (equivalent to 20-30 human years). LOW supplemented mice exhibited a rapid decline of serum 25-OH vitamin D levels by two weeks that remained between 11-15 ng/mL for all time points thereafter. After 12 months LOW mice displayed worse grip endurance (34.6 ± 14.1 versus 147.5 ± 50.6 seconds, p=0.001), uphill sprint speed (16.0 ± 1.0 versus 21.8 ± 2.4 meters/min, p=0.0007), and stride length (4.4 ± 0.3 versus 5.1 ± 0.3, p=0.002). LOW mice also showed less lean body mass after 8 months (57.5% ± 5.1% versus 64.5% ± 4.0%, p=0.023), but not after 12 months of supplementation, as well as greater protein expression of atrophy pathway gene atrogin‑1. Additionally, microRNA sequencing revealed differential expression of mIR‑26a in muscle tissue of LOW mice. These data suggest chronic vitamin D insufficiency may be an important factor contributing to functional decline and sarcopenia.
format Online
Article
Text
id pubmed-6046224
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-60462242018-07-17 Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice Seldeen, Kenneth L. Pang, Manhui Leiker, Merced M. Bard, Jonathan E. Rodríguez-Gonzalez, Maria Hernandez, Mireya Sheridan, Zachary Nowak, Norma Troen, Bruce R. Aging (Albany NY) Research Paper Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (STD, 1000 IU) or insufficient (LOW, 125 IU) vitamin D3/kg chow for 12 months (equivalent to 20-30 human years). LOW supplemented mice exhibited a rapid decline of serum 25-OH vitamin D levels by two weeks that remained between 11-15 ng/mL for all time points thereafter. After 12 months LOW mice displayed worse grip endurance (34.6 ± 14.1 versus 147.5 ± 50.6 seconds, p=0.001), uphill sprint speed (16.0 ± 1.0 versus 21.8 ± 2.4 meters/min, p=0.0007), and stride length (4.4 ± 0.3 versus 5.1 ± 0.3, p=0.002). LOW mice also showed less lean body mass after 8 months (57.5% ± 5.1% versus 64.5% ± 4.0%, p=0.023), but not after 12 months of supplementation, as well as greater protein expression of atrophy pathway gene atrogin‑1. Additionally, microRNA sequencing revealed differential expression of mIR‑26a in muscle tissue of LOW mice. These data suggest chronic vitamin D insufficiency may be an important factor contributing to functional decline and sarcopenia. Impact Journals 2018-06-14 /pmc/articles/PMC6046224/ /pubmed/29905532 http://dx.doi.org/10.18632/aging.101471 Text en Copyright © 2018 Seldeen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Seldeen, Kenneth L.
Pang, Manhui
Leiker, Merced M.
Bard, Jonathan E.
Rodríguez-Gonzalez, Maria
Hernandez, Mireya
Sheridan, Zachary
Nowak, Norma
Troen, Bruce R.
Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
title Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
title_full Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
title_fullStr Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
title_full_unstemmed Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
title_short Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
title_sort chronic vitamin d insufficiency impairs physical performance in c57bl/6j mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046224/
https://www.ncbi.nlm.nih.gov/pubmed/29905532
http://dx.doi.org/10.18632/aging.101471
work_keys_str_mv AT seldeenkennethl chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT pangmanhui chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT leikermercedm chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT bardjonathane chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT rodriguezgonzalezmaria chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT hernandezmireya chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT sheridanzachary chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT nowaknorma chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice
AT troenbrucer chronicvitamindinsufficiencyimpairsphysicalperformanceinc57bl6jmice