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Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (S...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046224/ https://www.ncbi.nlm.nih.gov/pubmed/29905532 http://dx.doi.org/10.18632/aging.101471 |
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author | Seldeen, Kenneth L. Pang, Manhui Leiker, Merced M. Bard, Jonathan E. Rodríguez-Gonzalez, Maria Hernandez, Mireya Sheridan, Zachary Nowak, Norma Troen, Bruce R. |
author_facet | Seldeen, Kenneth L. Pang, Manhui Leiker, Merced M. Bard, Jonathan E. Rodríguez-Gonzalez, Maria Hernandez, Mireya Sheridan, Zachary Nowak, Norma Troen, Bruce R. |
author_sort | Seldeen, Kenneth L. |
collection | PubMed |
description | Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (STD, 1000 IU) or insufficient (LOW, 125 IU) vitamin D3/kg chow for 12 months (equivalent to 20-30 human years). LOW supplemented mice exhibited a rapid decline of serum 25-OH vitamin D levels by two weeks that remained between 11-15 ng/mL for all time points thereafter. After 12 months LOW mice displayed worse grip endurance (34.6 ± 14.1 versus 147.5 ± 50.6 seconds, p=0.001), uphill sprint speed (16.0 ± 1.0 versus 21.8 ± 2.4 meters/min, p=0.0007), and stride length (4.4 ± 0.3 versus 5.1 ± 0.3, p=0.002). LOW mice also showed less lean body mass after 8 months (57.5% ± 5.1% versus 64.5% ± 4.0%, p=0.023), but not after 12 months of supplementation, as well as greater protein expression of atrophy pathway gene atrogin‑1. Additionally, microRNA sequencing revealed differential expression of mIR‑26a in muscle tissue of LOW mice. These data suggest chronic vitamin D insufficiency may be an important factor contributing to functional decline and sarcopenia. |
format | Online Article Text |
id | pubmed-6046224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-60462242018-07-17 Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice Seldeen, Kenneth L. Pang, Manhui Leiker, Merced M. Bard, Jonathan E. Rodríguez-Gonzalez, Maria Hernandez, Mireya Sheridan, Zachary Nowak, Norma Troen, Bruce R. Aging (Albany NY) Research Paper Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (STD, 1000 IU) or insufficient (LOW, 125 IU) vitamin D3/kg chow for 12 months (equivalent to 20-30 human years). LOW supplemented mice exhibited a rapid decline of serum 25-OH vitamin D levels by two weeks that remained between 11-15 ng/mL for all time points thereafter. After 12 months LOW mice displayed worse grip endurance (34.6 ± 14.1 versus 147.5 ± 50.6 seconds, p=0.001), uphill sprint speed (16.0 ± 1.0 versus 21.8 ± 2.4 meters/min, p=0.0007), and stride length (4.4 ± 0.3 versus 5.1 ± 0.3, p=0.002). LOW mice also showed less lean body mass after 8 months (57.5% ± 5.1% versus 64.5% ± 4.0%, p=0.023), but not after 12 months of supplementation, as well as greater protein expression of atrophy pathway gene atrogin‑1. Additionally, microRNA sequencing revealed differential expression of mIR‑26a in muscle tissue of LOW mice. These data suggest chronic vitamin D insufficiency may be an important factor contributing to functional decline and sarcopenia. Impact Journals 2018-06-14 /pmc/articles/PMC6046224/ /pubmed/29905532 http://dx.doi.org/10.18632/aging.101471 Text en Copyright © 2018 Seldeen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Seldeen, Kenneth L. Pang, Manhui Leiker, Merced M. Bard, Jonathan E. Rodríguez-Gonzalez, Maria Hernandez, Mireya Sheridan, Zachary Nowak, Norma Troen, Bruce R. Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice |
title | Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice |
title_full | Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice |
title_fullStr | Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice |
title_full_unstemmed | Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice |
title_short | Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice |
title_sort | chronic vitamin d insufficiency impairs physical performance in c57bl/6j mice |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046224/ https://www.ncbi.nlm.nih.gov/pubmed/29905532 http://dx.doi.org/10.18632/aging.101471 |
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