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Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy

Purpose: Unique physiochemical properties of Fe(2)O(3) nanoparticles make them great agents to serve as therapeutic and diagnostic nanoparticles (NPs). In this study, we developed gold coated Fe(2)O(3) nanoparticles for photothermal therapy of breast cancer cells. Methods: Fe(2)O(3) nanoparticles wa...

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Autores principales: Nassireslami, Ehsan, Ajdarzade, Morteza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046429/
https://www.ncbi.nlm.nih.gov/pubmed/30023321
http://dx.doi.org/10.15171/apb.2018.024
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author Nassireslami, Ehsan
Ajdarzade, Morteza
author_facet Nassireslami, Ehsan
Ajdarzade, Morteza
author_sort Nassireslami, Ehsan
collection PubMed
description Purpose: Unique physiochemical properties of Fe(2)O(3) nanoparticles make them great agents to serve as therapeutic and diagnostic nanoparticles (NPs). In this study, we developed gold coated Fe(2)O(3) nanoparticles for photothermal therapy of breast cancer cells. Methods: Fe(2)O(3) nanoparticles was prepared via microemulsion method and their surface was modified via gold. Differential light scattering (DLS) and transmission electron microscopy (TEM) methods were applied to evaluate physicochemical properties of NPs. Gold coated NP was further modified with MUC-1 aptamer as a targeting agent to increase drug delivery into the desired tissue. To evaluate cytotoxicity of prepared cells, MTT assay was employed. Targeting ability of aptamer modified NPs was assessed through confocal microscopy and flow cytometry method. Subsequently, MCF-7 and CHO cells were treated with aptamer modified NPs and were then irradiated via near infrared light (NIR) to produce heat. Results: The morphology of NPs was spherical and monodisperse with the size of 16 nm, which was confirmed via DLS and TEM. Confocal microscopy and flow cytometry results indicated that aptamer modified NPs had higher uptake compared to bare NPs. Finally, NIR exposure results revealed that higher uptake of NPs and application of NIR led to significant death of MCF-7 cells compared to CHO cells. Conclusion: To sum up, aptamer modified Fe(2)O(3) nanoparticles showed higher uptake by cancerous cells and led to eradication of cancerous cells after exposure to NIR light.
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spelling pubmed-60464292018-07-18 Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy Nassireslami, Ehsan Ajdarzade, Morteza Adv Pharm Bull Research Article Purpose: Unique physiochemical properties of Fe(2)O(3) nanoparticles make them great agents to serve as therapeutic and diagnostic nanoparticles (NPs). In this study, we developed gold coated Fe(2)O(3) nanoparticles for photothermal therapy of breast cancer cells. Methods: Fe(2)O(3) nanoparticles was prepared via microemulsion method and their surface was modified via gold. Differential light scattering (DLS) and transmission electron microscopy (TEM) methods were applied to evaluate physicochemical properties of NPs. Gold coated NP was further modified with MUC-1 aptamer as a targeting agent to increase drug delivery into the desired tissue. To evaluate cytotoxicity of prepared cells, MTT assay was employed. Targeting ability of aptamer modified NPs was assessed through confocal microscopy and flow cytometry method. Subsequently, MCF-7 and CHO cells were treated with aptamer modified NPs and were then irradiated via near infrared light (NIR) to produce heat. Results: The morphology of NPs was spherical and monodisperse with the size of 16 nm, which was confirmed via DLS and TEM. Confocal microscopy and flow cytometry results indicated that aptamer modified NPs had higher uptake compared to bare NPs. Finally, NIR exposure results revealed that higher uptake of NPs and application of NIR led to significant death of MCF-7 cells compared to CHO cells. Conclusion: To sum up, aptamer modified Fe(2)O(3) nanoparticles showed higher uptake by cancerous cells and led to eradication of cancerous cells after exposure to NIR light. Tabriz University of Medical Sciences 2018-06 2018-06-19 /pmc/articles/PMC6046429/ /pubmed/30023321 http://dx.doi.org/10.15171/apb.2018.024 Text en ©2018 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
spellingShingle Research Article
Nassireslami, Ehsan
Ajdarzade, Morteza
Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy
title Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy
title_full Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy
title_fullStr Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy
title_full_unstemmed Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy
title_short Gold Coated Superparamagnetic Iron Oxide Nanoparticles as Effective Nanoparticles to Eradicate Breast Cancer Cells via Photothermal Therapy
title_sort gold coated superparamagnetic iron oxide nanoparticles as effective nanoparticles to eradicate breast cancer cells via photothermal therapy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046429/
https://www.ncbi.nlm.nih.gov/pubmed/30023321
http://dx.doi.org/10.15171/apb.2018.024
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