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Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection

PURPOSE: The authors previously revealed the association of the follicular fluid (FF) volume with oolemma stretchability following the gonadotropin‐releasing hormone (GnRH) antagonist protocol during intracytoplasmic sperm injection (ICSI). However, the impact of the GnRH agonist protocol on oolemma...

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Autores principales: Inoue, Taketo, Taguchi, Sayumi, Hirao, Kayoko, Tsujimoto, Yoshiko, Yamamoto, Shuji, Uemura, Mikiko, Miyazaki, Kazunori, Yamashita, Yoshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046520/
https://www.ncbi.nlm.nih.gov/pubmed/30013430
http://dx.doi.org/10.1002/rmb2.12102
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author Inoue, Taketo
Taguchi, Sayumi
Hirao, Kayoko
Tsujimoto, Yoshiko
Yamamoto, Shuji
Uemura, Mikiko
Miyazaki, Kazunori
Yamashita, Yoshiki
author_facet Inoue, Taketo
Taguchi, Sayumi
Hirao, Kayoko
Tsujimoto, Yoshiko
Yamamoto, Shuji
Uemura, Mikiko
Miyazaki, Kazunori
Yamashita, Yoshiki
author_sort Inoue, Taketo
collection PubMed
description PURPOSE: The authors previously revealed the association of the follicular fluid (FF) volume with oolemma stretchability following the gonadotropin‐releasing hormone (GnRH) antagonist protocol during intracytoplasmic sperm injection (ICSI). However, the impact of the GnRH agonist protocol on oolemma stretchability remains unclear. METHODS: Data that were obtained from 74 ICSI cycles were reviewed retrospectively. Controlled ovarian stimulation was performed in accordance with the short GnRH agonist protocol. Each follicle was individually aspirated and assigned to one of six groups, according to the FF volume. The oolemma stretchability during ICSI was evaluated by using a mechanical stimulus for oolemma penetration; that is, oolemma penetration with or without aspiration (high vs low stretchability, respectively). RESULTS: The incidence of low oolemma stretchability was significantly higher in the <1.0 mL group than that in the ≥1.0 mL group. The normal fertilization rate was significantly lower in the <1.0 mL group than that in the 2.0‐<3.0 mL group. The rate of blastocyst development was lower in the <1.0 mL group than that in the 3.0‐<4.0 mL group. CONCLUSION: The FF volume potentially was associated with metaphase II oolemma stretchability, fertilization, and blastocyst development.
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spelling pubmed-60465202018-07-16 Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection Inoue, Taketo Taguchi, Sayumi Hirao, Kayoko Tsujimoto, Yoshiko Yamamoto, Shuji Uemura, Mikiko Miyazaki, Kazunori Yamashita, Yoshiki Reprod Med Biol Original Articles PURPOSE: The authors previously revealed the association of the follicular fluid (FF) volume with oolemma stretchability following the gonadotropin‐releasing hormone (GnRH) antagonist protocol during intracytoplasmic sperm injection (ICSI). However, the impact of the GnRH agonist protocol on oolemma stretchability remains unclear. METHODS: Data that were obtained from 74 ICSI cycles were reviewed retrospectively. Controlled ovarian stimulation was performed in accordance with the short GnRH agonist protocol. Each follicle was individually aspirated and assigned to one of six groups, according to the FF volume. The oolemma stretchability during ICSI was evaluated by using a mechanical stimulus for oolemma penetration; that is, oolemma penetration with or without aspiration (high vs low stretchability, respectively). RESULTS: The incidence of low oolemma stretchability was significantly higher in the <1.0 mL group than that in the ≥1.0 mL group. The normal fertilization rate was significantly lower in the <1.0 mL group than that in the 2.0‐<3.0 mL group. The rate of blastocyst development was lower in the <1.0 mL group than that in the 3.0‐<4.0 mL group. CONCLUSION: The FF volume potentially was associated with metaphase II oolemma stretchability, fertilization, and blastocyst development. John Wiley and Sons Inc. 2018-05-11 /pmc/articles/PMC6046520/ /pubmed/30013430 http://dx.doi.org/10.1002/rmb2.12102 Text en © 2018 The Authors Reproductive Medicine and Biology published by John Wiley & Sons Australia, Ltd on behalf of Japan Society for Reproductive Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Inoue, Taketo
Taguchi, Sayumi
Hirao, Kayoko
Tsujimoto, Yoshiko
Yamamoto, Shuji
Uemura, Mikiko
Miyazaki, Kazunori
Yamashita, Yoshiki
Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
title Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
title_full Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
title_fullStr Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
title_full_unstemmed Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
title_short Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
title_sort association of follicular fluid volume with membrane stretchability of human metaphase ii oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046520/
https://www.ncbi.nlm.nih.gov/pubmed/30013430
http://dx.doi.org/10.1002/rmb2.12102
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