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Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers
Lilium, the famous and significant cut flower, emits a variety of volatile organic compounds, which mainly contain monoterpenes, such as myrcene, (E)-β-ocimene, and linalool. To understand the molecular mechanism of monoterpene synthesis in Lilium, we cloned two potential genes in the methylerythrit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046550/ https://www.ncbi.nlm.nih.gov/pubmed/30038631 http://dx.doi.org/10.3389/fpls.2018.00909 |
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author | Zhang, Tengxun Sun, Ming Guo, Yanhong Shi, Xuejun Yang, Yongjuan Chen, Juntong Zheng, Tangchun Han, Yu Bao, Fei Ahmad, Sagheer |
author_facet | Zhang, Tengxun Sun, Ming Guo, Yanhong Shi, Xuejun Yang, Yongjuan Chen, Juntong Zheng, Tangchun Han, Yu Bao, Fei Ahmad, Sagheer |
author_sort | Zhang, Tengxun |
collection | PubMed |
description | Lilium, the famous and significant cut flower, emits a variety of volatile organic compounds, which mainly contain monoterpenes, such as myrcene, (E)-β-ocimene, and linalool. To understand the molecular mechanism of monoterpene synthesis in Lilium, we cloned two potential genes in the methylerythritol 4-phosphate pathway, namely LiDXS and LiDXR, from the strong-flavored oriental Lilium ‘Siberia’ using a homology-based PCR strategy. The expression levels of LiDXS and LiDXR were consistent with the emission and accumulation of monoterpenes in different floral organs and during the floral development, indicating that these two genes may play key roles in monoterpene synthesis. Subcellular localization demonstrated that LiDXS and LiDXR are expressed in the chloroplasts. Ectopic expression in transgenic tobacco suggested that the flowers of LiDXS and LiDXR transgenic lines accumulated substantially more diterpene, sclareol, compared to the plants transformed with empty vector. Surprisingly, increased content of the monoterpene, linalool and sesquiterpene, caryophyllene, were detected in the LiDXR transgenic lines, whereas the emission of caryophyllene, increased in one of the LiDXS transgenic tobacco lines, indicating that these two genes play significant roles in the synthesis of floral volatiles in the transgenic plants. These results demonstrate that LiDXR can contribute to monoterpene biosynthesis in Lilium ‘Siberia’; however, the role of LiDXS in the biosynthesis of monoterpenes needs further study. |
format | Online Article Text |
id | pubmed-6046550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60465502018-07-23 Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers Zhang, Tengxun Sun, Ming Guo, Yanhong Shi, Xuejun Yang, Yongjuan Chen, Juntong Zheng, Tangchun Han, Yu Bao, Fei Ahmad, Sagheer Front Plant Sci Plant Science Lilium, the famous and significant cut flower, emits a variety of volatile organic compounds, which mainly contain monoterpenes, such as myrcene, (E)-β-ocimene, and linalool. To understand the molecular mechanism of monoterpene synthesis in Lilium, we cloned two potential genes in the methylerythritol 4-phosphate pathway, namely LiDXS and LiDXR, from the strong-flavored oriental Lilium ‘Siberia’ using a homology-based PCR strategy. The expression levels of LiDXS and LiDXR were consistent with the emission and accumulation of monoterpenes in different floral organs and during the floral development, indicating that these two genes may play key roles in monoterpene synthesis. Subcellular localization demonstrated that LiDXS and LiDXR are expressed in the chloroplasts. Ectopic expression in transgenic tobacco suggested that the flowers of LiDXS and LiDXR transgenic lines accumulated substantially more diterpene, sclareol, compared to the plants transformed with empty vector. Surprisingly, increased content of the monoterpene, linalool and sesquiterpene, caryophyllene, were detected in the LiDXR transgenic lines, whereas the emission of caryophyllene, increased in one of the LiDXS transgenic tobacco lines, indicating that these two genes play significant roles in the synthesis of floral volatiles in the transgenic plants. These results demonstrate that LiDXR can contribute to monoterpene biosynthesis in Lilium ‘Siberia’; however, the role of LiDXS in the biosynthesis of monoterpenes needs further study. Frontiers Media S.A. 2018-07-09 /pmc/articles/PMC6046550/ /pubmed/30038631 http://dx.doi.org/10.3389/fpls.2018.00909 Text en Copyright © 2018 Zhang, Sun, Guo, Shi, Yang, Chen, Zheng, Han, Bao and Ahmad. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Tengxun Sun, Ming Guo, Yanhong Shi, Xuejun Yang, Yongjuan Chen, Juntong Zheng, Tangchun Han, Yu Bao, Fei Ahmad, Sagheer Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers |
title | Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers |
title_full | Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers |
title_fullStr | Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers |
title_full_unstemmed | Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers |
title_short | Overexpression of LiDXS and LiDXR From Lily (Lilium ‘Siberia’) Enhances the Terpenoid Content in Tobacco Flowers |
title_sort | overexpression of lidxs and lidxr from lily (lilium ‘siberia’) enhances the terpenoid content in tobacco flowers |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046550/ https://www.ncbi.nlm.nih.gov/pubmed/30038631 http://dx.doi.org/10.3389/fpls.2018.00909 |
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