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Targeted metabolomic approach in men with carotid plaque

BACKGROUND: The aim of the study was to analyse the presence of several metabolites related to atherosclerosis in the plasma of patients with unstable carotid plaque and in the plasma of healthy subjects. MATERIALS AND METHODS: We included 20 patients who had undergone carotid endarterectomy and 20...

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Autores principales: Auguet, Teresa, Aragonès, Gemma, Colom, Marina, Aguilar, Carmen, Martín-Paredero, Vicente, Canela, Núria, Ruyra, Xavier, Richart, Cristóbal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047792/
https://www.ncbi.nlm.nih.gov/pubmed/30011297
http://dx.doi.org/10.1371/journal.pone.0200547
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author Auguet, Teresa
Aragonès, Gemma
Colom, Marina
Aguilar, Carmen
Martín-Paredero, Vicente
Canela, Núria
Ruyra, Xavier
Richart, Cristóbal
author_facet Auguet, Teresa
Aragonès, Gemma
Colom, Marina
Aguilar, Carmen
Martín-Paredero, Vicente
Canela, Núria
Ruyra, Xavier
Richart, Cristóbal
author_sort Auguet, Teresa
collection PubMed
description BACKGROUND: The aim of the study was to analyse the presence of several metabolites related to atherosclerosis in the plasma of patients with unstable carotid plaque and in the plasma of healthy subjects. MATERIALS AND METHODS: We included 20 patients who had undergone carotid endarterectomy and 20 healthy subjects as a control group. All the subjects recruited were male. We used a metabolomic approach with liquid chromatography coupled to mass spectrometry to evaluate plasma metabolite levels in the metabolic pathway involved in the progression of atherosclerotic plaque. RESULTS: We observed that circulating levels of 20-HETE were significantly higher in patients with atheroma plaque than in healthy subjects (p = 0.018). No differences were found with regard to the other metabolites analysed. We also conducted a random forest analysis and found that 20-HETE was the main differentiator in the list of selected metabolites. In addition, plasma levels of 20-HETE correlated positively with body mass index (r = 0.427, p = 0.007) and diastolic blood pressure (r = 0.365, p = 0.028). CONCLUSION: This study confirms that of all the molecules studied only 20-HETE is related to carotid plaque. Further studies are needed to compare patients with stable carotid plaque vs. patients with unstable carotid plaque in order to confirm that 20-HETE could be a potential factor related to carotid plaque.
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spelling pubmed-60477922018-07-26 Targeted metabolomic approach in men with carotid plaque Auguet, Teresa Aragonès, Gemma Colom, Marina Aguilar, Carmen Martín-Paredero, Vicente Canela, Núria Ruyra, Xavier Richart, Cristóbal PLoS One Research Article BACKGROUND: The aim of the study was to analyse the presence of several metabolites related to atherosclerosis in the plasma of patients with unstable carotid plaque and in the plasma of healthy subjects. MATERIALS AND METHODS: We included 20 patients who had undergone carotid endarterectomy and 20 healthy subjects as a control group. All the subjects recruited were male. We used a metabolomic approach with liquid chromatography coupled to mass spectrometry to evaluate plasma metabolite levels in the metabolic pathway involved in the progression of atherosclerotic plaque. RESULTS: We observed that circulating levels of 20-HETE were significantly higher in patients with atheroma plaque than in healthy subjects (p = 0.018). No differences were found with regard to the other metabolites analysed. We also conducted a random forest analysis and found that 20-HETE was the main differentiator in the list of selected metabolites. In addition, plasma levels of 20-HETE correlated positively with body mass index (r = 0.427, p = 0.007) and diastolic blood pressure (r = 0.365, p = 0.028). CONCLUSION: This study confirms that of all the molecules studied only 20-HETE is related to carotid plaque. Further studies are needed to compare patients with stable carotid plaque vs. patients with unstable carotid plaque in order to confirm that 20-HETE could be a potential factor related to carotid plaque. Public Library of Science 2018-07-16 /pmc/articles/PMC6047792/ /pubmed/30011297 http://dx.doi.org/10.1371/journal.pone.0200547 Text en © 2018 Auguet et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Auguet, Teresa
Aragonès, Gemma
Colom, Marina
Aguilar, Carmen
Martín-Paredero, Vicente
Canela, Núria
Ruyra, Xavier
Richart, Cristóbal
Targeted metabolomic approach in men with carotid plaque
title Targeted metabolomic approach in men with carotid plaque
title_full Targeted metabolomic approach in men with carotid plaque
title_fullStr Targeted metabolomic approach in men with carotid plaque
title_full_unstemmed Targeted metabolomic approach in men with carotid plaque
title_short Targeted metabolomic approach in men with carotid plaque
title_sort targeted metabolomic approach in men with carotid plaque
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047792/
https://www.ncbi.nlm.nih.gov/pubmed/30011297
http://dx.doi.org/10.1371/journal.pone.0200547
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