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MiR-150-3p targets SP1 and suppresses the growth of glioma cells

Glioma has been considered as one of the most prevalent and common malignancy of the nervous system; however, the underlying mechanisms that are responsible for the occurrence and development of glioma still remain largely unknown. Amounting evidence highlights the critical regulatory function of mi...

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Detalles Bibliográficos
Autores principales: Tan, Zhigang, Jia, Jiaoying, Jiang, Yugang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048207/
https://www.ncbi.nlm.nih.gov/pubmed/29654167
http://dx.doi.org/10.1042/BSR20180019
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author Tan, Zhigang
Jia, Jiaoying
Jiang, Yugang
author_facet Tan, Zhigang
Jia, Jiaoying
Jiang, Yugang
author_sort Tan, Zhigang
collection PubMed
description Glioma has been considered as one of the most prevalent and common malignancy of the nervous system; however, the underlying mechanisms that are responsible for the occurrence and development of glioma still remain largely unknown. Amounting evidence highlights the critical regulatory function of miRNAs in carcinogenesis. Here, we showed that the expression of miR-150-3p was significantly decreased in glioma tissues and cell lines. Suppressed expression of miR-150-3p was associated with the lymph node metastasis of the glioma patients. Overexpression of miR-150-3p significantly inhibited the proliferation of glioma cells. Molecular study uncovered that the transcription factor specificity protein 1 (SP1) was identified as one of the targets of miR-150-3p. Highly expressed miR-150-3p in glioma cells significantly decreased both the mRNA and protein levels of SP1. Consistently, the abundance of phosphatase and tension homolog deleted on chromosome ten (PTEN), a negative downstream target of SP1, was increased with the ectopic miR-150-3p. Collectively, these results suggested that miR-150-3p suppressed the growth of glioma cells partially via regulating SP1 and possibly PTEN.
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spelling pubmed-60482072018-07-23 MiR-150-3p targets SP1 and suppresses the growth of glioma cells Tan, Zhigang Jia, Jiaoying Jiang, Yugang Biosci Rep Research Articles Glioma has been considered as one of the most prevalent and common malignancy of the nervous system; however, the underlying mechanisms that are responsible for the occurrence and development of glioma still remain largely unknown. Amounting evidence highlights the critical regulatory function of miRNAs in carcinogenesis. Here, we showed that the expression of miR-150-3p was significantly decreased in glioma tissues and cell lines. Suppressed expression of miR-150-3p was associated with the lymph node metastasis of the glioma patients. Overexpression of miR-150-3p significantly inhibited the proliferation of glioma cells. Molecular study uncovered that the transcription factor specificity protein 1 (SP1) was identified as one of the targets of miR-150-3p. Highly expressed miR-150-3p in glioma cells significantly decreased both the mRNA and protein levels of SP1. Consistently, the abundance of phosphatase and tension homolog deleted on chromosome ten (PTEN), a negative downstream target of SP1, was increased with the ectopic miR-150-3p. Collectively, these results suggested that miR-150-3p suppressed the growth of glioma cells partially via regulating SP1 and possibly PTEN. Portland Press Ltd. 2018-05-15 /pmc/articles/PMC6048207/ /pubmed/29654167 http://dx.doi.org/10.1042/BSR20180019 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Tan, Zhigang
Jia, Jiaoying
Jiang, Yugang
MiR-150-3p targets SP1 and suppresses the growth of glioma cells
title MiR-150-3p targets SP1 and suppresses the growth of glioma cells
title_full MiR-150-3p targets SP1 and suppresses the growth of glioma cells
title_fullStr MiR-150-3p targets SP1 and suppresses the growth of glioma cells
title_full_unstemmed MiR-150-3p targets SP1 and suppresses the growth of glioma cells
title_short MiR-150-3p targets SP1 and suppresses the growth of glioma cells
title_sort mir-150-3p targets sp1 and suppresses the growth of glioma cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048207/
https://www.ncbi.nlm.nih.gov/pubmed/29654167
http://dx.doi.org/10.1042/BSR20180019
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