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The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese

Many long non-coding RNAs (lncRNAs), including lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), are involved in various cardiac diseases. We evaluated the effects of tag single nucleotide polymorphisms (tag-SNPs) on MALAT1 gene in a Chinese population of children with congenit...

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Autores principales: Li, Qian, Zhu, Wenying, Zhang, Bei, Wu, Yiping, Yan, Sen, Yuan, Yufeng, Zhang, Haiyan, Li, Jie, Sun, Kai, Wang, Hua, Yu, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048208/
https://www.ncbi.nlm.nih.gov/pubmed/29559566
http://dx.doi.org/10.1042/BSR20171381
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author Li, Qian
Zhu, Wenying
Zhang, Bei
Wu, Yiping
Yan, Sen
Yuan, Yufeng
Zhang, Haiyan
Li, Jie
Sun, Kai
Wang, Hua
Yu, Tingting
author_facet Li, Qian
Zhu, Wenying
Zhang, Bei
Wu, Yiping
Yan, Sen
Yuan, Yufeng
Zhang, Haiyan
Li, Jie
Sun, Kai
Wang, Hua
Yu, Tingting
author_sort Li, Qian
collection PubMed
description Many long non-coding RNAs (lncRNAs), including lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), are involved in various cardiac diseases. We evaluated the effects of tag single nucleotide polymorphisms (tag-SNPs) on MALAT1 gene in a Chinese population of children with congenital heart disease (CHD). In the present study, 713 CHD patients and 730 gender- and age-matched children without CHD were genotyped for MALAT1 tag-SNPs rs11227209, rs619586, and rs3200401. Further investigation of SNP’s function was performed by luciferase assay. Statistical analyses, including uni- and multivariate logistic regression were performed to quantitate the association between these tag SNPs and CHD. We discovered that MALAT1 rs619586 GG allele was significantly associated with lower risk of CHD (odds ratio (OR) = 0.77, 95% confidence interval (CI) = 0.59–0.92, P=0.014) in additive model. Functional investigation indicated that G allele of rs619586 could trigger higher expression of MALAT1. We demonstrated that the functional MALAT1 polymorphism rs619586 A>G was significantly associated with CHD susceptibility in Chinese population, potentially via regulating MALAT1 expression.
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spelling pubmed-60482082018-07-23 The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese Li, Qian Zhu, Wenying Zhang, Bei Wu, Yiping Yan, Sen Yuan, Yufeng Zhang, Haiyan Li, Jie Sun, Kai Wang, Hua Yu, Tingting Biosci Rep Research Articles Many long non-coding RNAs (lncRNAs), including lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), are involved in various cardiac diseases. We evaluated the effects of tag single nucleotide polymorphisms (tag-SNPs) on MALAT1 gene in a Chinese population of children with congenital heart disease (CHD). In the present study, 713 CHD patients and 730 gender- and age-matched children without CHD were genotyped for MALAT1 tag-SNPs rs11227209, rs619586, and rs3200401. Further investigation of SNP’s function was performed by luciferase assay. Statistical analyses, including uni- and multivariate logistic regression were performed to quantitate the association between these tag SNPs and CHD. We discovered that MALAT1 rs619586 GG allele was significantly associated with lower risk of CHD (odds ratio (OR) = 0.77, 95% confidence interval (CI) = 0.59–0.92, P=0.014) in additive model. Functional investigation indicated that G allele of rs619586 could trigger higher expression of MALAT1. We demonstrated that the functional MALAT1 polymorphism rs619586 A>G was significantly associated with CHD susceptibility in Chinese population, potentially via regulating MALAT1 expression. Portland Press Ltd. 2018-05-22 /pmc/articles/PMC6048208/ /pubmed/29559566 http://dx.doi.org/10.1042/BSR20171381 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Li, Qian
Zhu, Wenying
Zhang, Bei
Wu, Yiping
Yan, Sen
Yuan, Yufeng
Zhang, Haiyan
Li, Jie
Sun, Kai
Wang, Hua
Yu, Tingting
The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese
title The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese
title_full The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese
title_fullStr The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese
title_full_unstemmed The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese
title_short The MALAT1 gene polymorphism and its relationship with the onset of congenital heart disease in Chinese
title_sort malat1 gene polymorphism and its relationship with the onset of congenital heart disease in chinese
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048208/
https://www.ncbi.nlm.nih.gov/pubmed/29559566
http://dx.doi.org/10.1042/BSR20171381
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