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Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis

The author undertook PCR-founded genetic platform to investigate the hierarchical dendrogram of Euclidean genetic distances of one razor clam population, particularly for Solen corneus, which was further associated with those of the other clam population, by engaging with the precisely designed olig...

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Autor principal: Yoon, Jong-Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Developmental Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048308/
https://www.ncbi.nlm.nih.gov/pubmed/30023469
http://dx.doi.org/10.12717/DR.2018.22.2.193
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author Yoon, Jong-Man
author_facet Yoon, Jong-Man
author_sort Yoon, Jong-Man
collection PubMed
description The author undertook PCR-founded genetic platform to investigate the hierarchical dendrogram of Euclidean genetic distances of one razor clam population, particularly for Solen corneus, which was further associated with those of the other clam population, by engaging with the precisely designed oligonucleotide primer sets. Seven oligonucleotides primers were used producing a total of 639 counted bands in population A and 595 in population B, respectively, ranging in size of DNA fragments from larger than approximately 50 bp to less than 1,100 bp. Their primers generated 39 specific fragments (6.10%) in population A and 47 (7.90%) in population B, respectively Comparatively, individuals of one razor clam population were fairly related to that of the other clam population, as shown in the hierarchical dendrogram of Euclidean genetic distances. The analysis of genetic variation between razor clam populations could offer important statistics for fisheries and mariculture. Generally the results showed specific and/or conserved genetic loci between razor clam populations. Specific markers established by the author will be valuable for the genetic analysis, species protection and increase of razor clam individuals in coastal region of the Korean Peninsula.
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spelling pubmed-60483082018-07-18 Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis Yoon, Jong-Man Dev Reprod Short Communication The author undertook PCR-founded genetic platform to investigate the hierarchical dendrogram of Euclidean genetic distances of one razor clam population, particularly for Solen corneus, which was further associated with those of the other clam population, by engaging with the precisely designed oligonucleotide primer sets. Seven oligonucleotides primers were used producing a total of 639 counted bands in population A and 595 in population B, respectively, ranging in size of DNA fragments from larger than approximately 50 bp to less than 1,100 bp. Their primers generated 39 specific fragments (6.10%) in population A and 47 (7.90%) in population B, respectively Comparatively, individuals of one razor clam population were fairly related to that of the other clam population, as shown in the hierarchical dendrogram of Euclidean genetic distances. The analysis of genetic variation between razor clam populations could offer important statistics for fisheries and mariculture. Generally the results showed specific and/or conserved genetic loci between razor clam populations. Specific markers established by the author will be valuable for the genetic analysis, species protection and increase of razor clam individuals in coastal region of the Korean Peninsula. Korean Society of Developmental Biology 2018-06 2018-06-30 /pmc/articles/PMC6048308/ /pubmed/30023469 http://dx.doi.org/10.12717/DR.2018.22.2.193 Text en © Copyright 2018 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Yoon, Jong-Man
Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis
title Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis
title_full Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis
title_fullStr Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis
title_full_unstemmed Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis
title_short Genetic Variations of Intra- and between-razor Clam Solen corneus Population Identified by PCR Analysis
title_sort genetic variations of intra- and between-razor clam solen corneus population identified by pcr analysis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048308/
https://www.ncbi.nlm.nih.gov/pubmed/30023469
http://dx.doi.org/10.12717/DR.2018.22.2.193
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