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Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species

Pseudomonas syringae employs a battery of type three secretion effectors to subvert plant immune responses. In turn, plants have developed receptors that recognize some of the bacterial effectors. Two strain-specific HopQ1 effector variants (for Hrp outer protein Q) from the pathovars phaseolicola 1...

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Autores principales: Zembek, Patrycja, Danilecka, Aleksandra, Hoser, Rafał, Eschen-Lippold, Lennart, Benicka, Marta, Grech-Baran, Marta, Rymaszewski, Wojciech, Barymow-Filoniuk, Izabela, Morgiewicz, Karolina, Kwiatkowski, Jakub, Piechocki, Marcin, Poznanski, Jaroslaw, Lee, Justin, Hennig, Jacek, Krzymowska, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048448/
https://www.ncbi.nlm.nih.gov/pubmed/30042777
http://dx.doi.org/10.3389/fpls.2018.00978
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author Zembek, Patrycja
Danilecka, Aleksandra
Hoser, Rafał
Eschen-Lippold, Lennart
Benicka, Marta
Grech-Baran, Marta
Rymaszewski, Wojciech
Barymow-Filoniuk, Izabela
Morgiewicz, Karolina
Kwiatkowski, Jakub
Piechocki, Marcin
Poznanski, Jaroslaw
Lee, Justin
Hennig, Jacek
Krzymowska, Magdalena
author_facet Zembek, Patrycja
Danilecka, Aleksandra
Hoser, Rafał
Eschen-Lippold, Lennart
Benicka, Marta
Grech-Baran, Marta
Rymaszewski, Wojciech
Barymow-Filoniuk, Izabela
Morgiewicz, Karolina
Kwiatkowski, Jakub
Piechocki, Marcin
Poznanski, Jaroslaw
Lee, Justin
Hennig, Jacek
Krzymowska, Magdalena
author_sort Zembek, Patrycja
collection PubMed
description Pseudomonas syringae employs a battery of type three secretion effectors to subvert plant immune responses. In turn, plants have developed receptors that recognize some of the bacterial effectors. Two strain-specific HopQ1 effector variants (for Hrp outer protein Q) from the pathovars phaseolicola 1448A (Pph) and tomato DC3000 (Pto) showed considerable differences in their ability to evoke disease symptoms in Nicotiana benthamiana. Surprisingly, the variants differ by only six amino acids located mostly in the N-terminal disordered region of HopQ1. We found that the presence of serine 87 and leucine 91 renders PtoHopQ1 susceptible to N-terminal processing by plant proteases. Substitutions at these two positions did not strongly affect PtoHopQ1 virulence properties in a susceptible host but they reduced bacterial growth and accelerated onset of cell death in a resistant host, suggesting that N-terminal mutations rendered PtoHopQ1 susceptible to processing in planta and, thus, represent a mechanism of recognition avoidance. Furthermore, we found that co-expression of HopR1, another effector encoded within the same gene cluster masks HopQ1 recognition in a strain-dependent manner. Together, these data suggest that HopQ1 is under high host-pathogen co-evolutionary selection pressure and P. syringae may have evolved differential effector processing or masking as two independent strategies to evade HopQ1 recognition, thus revealing another level of complexity in plant – microbe interactions.
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spelling pubmed-60484482018-07-24 Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species Zembek, Patrycja Danilecka, Aleksandra Hoser, Rafał Eschen-Lippold, Lennart Benicka, Marta Grech-Baran, Marta Rymaszewski, Wojciech Barymow-Filoniuk, Izabela Morgiewicz, Karolina Kwiatkowski, Jakub Piechocki, Marcin Poznanski, Jaroslaw Lee, Justin Hennig, Jacek Krzymowska, Magdalena Front Plant Sci Plant Science Pseudomonas syringae employs a battery of type three secretion effectors to subvert plant immune responses. In turn, plants have developed receptors that recognize some of the bacterial effectors. Two strain-specific HopQ1 effector variants (for Hrp outer protein Q) from the pathovars phaseolicola 1448A (Pph) and tomato DC3000 (Pto) showed considerable differences in their ability to evoke disease symptoms in Nicotiana benthamiana. Surprisingly, the variants differ by only six amino acids located mostly in the N-terminal disordered region of HopQ1. We found that the presence of serine 87 and leucine 91 renders PtoHopQ1 susceptible to N-terminal processing by plant proteases. Substitutions at these two positions did not strongly affect PtoHopQ1 virulence properties in a susceptible host but they reduced bacterial growth and accelerated onset of cell death in a resistant host, suggesting that N-terminal mutations rendered PtoHopQ1 susceptible to processing in planta and, thus, represent a mechanism of recognition avoidance. Furthermore, we found that co-expression of HopR1, another effector encoded within the same gene cluster masks HopQ1 recognition in a strain-dependent manner. Together, these data suggest that HopQ1 is under high host-pathogen co-evolutionary selection pressure and P. syringae may have evolved differential effector processing or masking as two independent strategies to evade HopQ1 recognition, thus revealing another level of complexity in plant – microbe interactions. Frontiers Media S.A. 2018-07-10 /pmc/articles/PMC6048448/ /pubmed/30042777 http://dx.doi.org/10.3389/fpls.2018.00978 Text en Copyright © 2018 Zembek, Danilecka, Hoser, Eschen-Lippold, Benicka, Grech-Baran, Rymaszewski, Barymow-Filoniuk, Morgiewicz, Kwiatkowski, Piechocki, Poznanski, Lee, Hennig and Krzymowska. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zembek, Patrycja
Danilecka, Aleksandra
Hoser, Rafał
Eschen-Lippold, Lennart
Benicka, Marta
Grech-Baran, Marta
Rymaszewski, Wojciech
Barymow-Filoniuk, Izabela
Morgiewicz, Karolina
Kwiatkowski, Jakub
Piechocki, Marcin
Poznanski, Jaroslaw
Lee, Justin
Hennig, Jacek
Krzymowska, Magdalena
Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
title Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
title_full Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
title_fullStr Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
title_full_unstemmed Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
title_short Two Strategies of Pseudomonas syringae to Avoid Recognition of the HopQ1 Effector in Nicotiana Species
title_sort two strategies of pseudomonas syringae to avoid recognition of the hopq1 effector in nicotiana species
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048448/
https://www.ncbi.nlm.nih.gov/pubmed/30042777
http://dx.doi.org/10.3389/fpls.2018.00978
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