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Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites

Tetranychus urticae (two-spotted spider mite) is a striking example of polyphagy among herbivores with an extreme record of pesticide resistance and one of the most significant pests in agriculture. The T. urticae genome contains a large number of cysteine- and serine-proteases indicating their impo...

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Autores principales: Arnaiz, Ana, Talavera-Mateo, Lucia, Gonzalez-Melendi, Pablo, Martinez, Manuel, Diaz, Isabel, Santamaria, M. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048452/
https://www.ncbi.nlm.nih.gov/pubmed/30042779
http://dx.doi.org/10.3389/fpls.2018.00986
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author Arnaiz, Ana
Talavera-Mateo, Lucia
Gonzalez-Melendi, Pablo
Martinez, Manuel
Diaz, Isabel
Santamaria, M. E.
author_facet Arnaiz, Ana
Talavera-Mateo, Lucia
Gonzalez-Melendi, Pablo
Martinez, Manuel
Diaz, Isabel
Santamaria, M. E.
author_sort Arnaiz, Ana
collection PubMed
description Tetranychus urticae (two-spotted spider mite) is a striking example of polyphagy among herbivores with an extreme record of pesticide resistance and one of the most significant pests in agriculture. The T. urticae genome contains a large number of cysteine- and serine-proteases indicating their importance in the spider mite physiology. This work is focused on the potential role of the Kunitz trypsin inhibitor (KTI) family on plant defense responses against spider mites. The molecular characterization of two of these genes, AtKTI4 and AtKTI5, combined with feeding bioassays using T-DNA insertion lines for both genes was carried out. Spider mite performance assays showed that independent KTI silencing Arabidopsis lines conferred higher susceptibility to T. urticae than WT plants. Additionally, transient overexpression of these inhibitors in Nicotiana benthamiana demonstrated their ability to inhibit not only serine- but also cysteine-proteases, indicating the bifunctional inhibitory role against both types of enzymes. These inhibitory properties could be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the spider mite gut, as well as in other proteolytic processes.
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spelling pubmed-60484522018-07-24 Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites Arnaiz, Ana Talavera-Mateo, Lucia Gonzalez-Melendi, Pablo Martinez, Manuel Diaz, Isabel Santamaria, M. E. Front Plant Sci Plant Science Tetranychus urticae (two-spotted spider mite) is a striking example of polyphagy among herbivores with an extreme record of pesticide resistance and one of the most significant pests in agriculture. The T. urticae genome contains a large number of cysteine- and serine-proteases indicating their importance in the spider mite physiology. This work is focused on the potential role of the Kunitz trypsin inhibitor (KTI) family on plant defense responses against spider mites. The molecular characterization of two of these genes, AtKTI4 and AtKTI5, combined with feeding bioassays using T-DNA insertion lines for both genes was carried out. Spider mite performance assays showed that independent KTI silencing Arabidopsis lines conferred higher susceptibility to T. urticae than WT plants. Additionally, transient overexpression of these inhibitors in Nicotiana benthamiana demonstrated their ability to inhibit not only serine- but also cysteine-proteases, indicating the bifunctional inhibitory role against both types of enzymes. These inhibitory properties could be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the spider mite gut, as well as in other proteolytic processes. Frontiers Media S.A. 2018-07-10 /pmc/articles/PMC6048452/ /pubmed/30042779 http://dx.doi.org/10.3389/fpls.2018.00986 Text en Copyright © 2018 Arnaiz, Talavera-Mateo, Gonzalez-Melendi, Martinez, Diaz and Santamaria. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Arnaiz, Ana
Talavera-Mateo, Lucia
Gonzalez-Melendi, Pablo
Martinez, Manuel
Diaz, Isabel
Santamaria, M. E.
Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
title Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
title_full Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
title_fullStr Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
title_full_unstemmed Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
title_short Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
title_sort arabidopsis kunitz trypsin inhibitors in defense against spider mites
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048452/
https://www.ncbi.nlm.nih.gov/pubmed/30042779
http://dx.doi.org/10.3389/fpls.2018.00986
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