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Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice

Exacerbations in Chronic obstructive pulmonary disease (COPD) are often accompanied by pulmonary and systemic inflammation, and are associated with an increased susceptibility to weight loss and muscle wasting. As the emphysematous phenotype in COPD appears prone to skeletal muscle wasting, the aims...

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Autores principales: Ceelen, Judith J. M., Schols, Annemie M. W. J., Kneppers, Anita E. M., Rosenbrand, Roger P. H. A., Drożdż, Magda M., van Hoof, Stefan J., de Theije, Chiel C., Kelders, Marco C. J. M., Verhaegen, Frank, Langen, Ramon C. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050248/
https://www.ncbi.nlm.nih.gov/pubmed/30018383
http://dx.doi.org/10.1038/s41598-018-28579-4
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author Ceelen, Judith J. M.
Schols, Annemie M. W. J.
Kneppers, Anita E. M.
Rosenbrand, Roger P. H. A.
Drożdż, Magda M.
van Hoof, Stefan J.
de Theije, Chiel C.
Kelders, Marco C. J. M.
Verhaegen, Frank
Langen, Ramon C. J.
author_facet Ceelen, Judith J. M.
Schols, Annemie M. W. J.
Kneppers, Anita E. M.
Rosenbrand, Roger P. H. A.
Drożdż, Magda M.
van Hoof, Stefan J.
de Theije, Chiel C.
Kelders, Marco C. J. M.
Verhaegen, Frank
Langen, Ramon C. J.
author_sort Ceelen, Judith J. M.
collection PubMed
description Exacerbations in Chronic obstructive pulmonary disease (COPD) are often accompanied by pulmonary and systemic inflammation, and are associated with an increased susceptibility to weight loss and muscle wasting. As the emphysematous phenotype in COPD appears prone to skeletal muscle wasting, the aims of this study were to evaluate in emphysematous compared to control mice following repetitive exacerbations (1) changes in muscle mass and strength and, (2) whether muscle mass recovery and its underlying processes are impaired. Emphysema was induced by intra-tracheal (IT) elastase instillations, followed by three weekly IT-LPS instillations to mimic repetitive exacerbations. Loss of muscle mass and strength were measured, and related to analyses of muscle protein turnover and myogenesis signaling in tissue collected during and following recovery. Emphysematous mice showed impaired muscle mass recovery in response to pulmonary inflammation-induced muscle atrophy. Proteolysis and protein synthesis signaling remained significantly higher in emphysematous mice during recovery from LPS. Myogenic signaling in skeletal muscle was altered, and fusion capacity of cultured muscle cells treated with plasma derived from LPS-treated emphysematous mice was significantly decreased. In conclusion, repetitive cycles of pulmonary inflammation elicit sustained muscle wasting in emphysematous mice due to impaired muscle mass recovery, which is accompanied by aberrant myogenesis.
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spelling pubmed-60502482018-07-19 Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice Ceelen, Judith J. M. Schols, Annemie M. W. J. Kneppers, Anita E. M. Rosenbrand, Roger P. H. A. Drożdż, Magda M. van Hoof, Stefan J. de Theije, Chiel C. Kelders, Marco C. J. M. Verhaegen, Frank Langen, Ramon C. J. Sci Rep Article Exacerbations in Chronic obstructive pulmonary disease (COPD) are often accompanied by pulmonary and systemic inflammation, and are associated with an increased susceptibility to weight loss and muscle wasting. As the emphysematous phenotype in COPD appears prone to skeletal muscle wasting, the aims of this study were to evaluate in emphysematous compared to control mice following repetitive exacerbations (1) changes in muscle mass and strength and, (2) whether muscle mass recovery and its underlying processes are impaired. Emphysema was induced by intra-tracheal (IT) elastase instillations, followed by three weekly IT-LPS instillations to mimic repetitive exacerbations. Loss of muscle mass and strength were measured, and related to analyses of muscle protein turnover and myogenesis signaling in tissue collected during and following recovery. Emphysematous mice showed impaired muscle mass recovery in response to pulmonary inflammation-induced muscle atrophy. Proteolysis and protein synthesis signaling remained significantly higher in emphysematous mice during recovery from LPS. Myogenic signaling in skeletal muscle was altered, and fusion capacity of cultured muscle cells treated with plasma derived from LPS-treated emphysematous mice was significantly decreased. In conclusion, repetitive cycles of pulmonary inflammation elicit sustained muscle wasting in emphysematous mice due to impaired muscle mass recovery, which is accompanied by aberrant myogenesis. Nature Publishing Group UK 2018-07-17 /pmc/articles/PMC6050248/ /pubmed/30018383 http://dx.doi.org/10.1038/s41598-018-28579-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ceelen, Judith J. M.
Schols, Annemie M. W. J.
Kneppers, Anita E. M.
Rosenbrand, Roger P. H. A.
Drożdż, Magda M.
van Hoof, Stefan J.
de Theije, Chiel C.
Kelders, Marco C. J. M.
Verhaegen, Frank
Langen, Ramon C. J.
Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
title Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
title_full Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
title_fullStr Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
title_full_unstemmed Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
title_short Altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
title_sort altered protein turnover signaling and myogenesis during impaired recovery of inflammation-induced muscle atrophy in emphysematous mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050248/
https://www.ncbi.nlm.nih.gov/pubmed/30018383
http://dx.doi.org/10.1038/s41598-018-28579-4
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