Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

OBJECTIVE: Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere...

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Detalles Bibliográficos
Autores principales: Kargarpour Kamakoli, Mansour, Khanipour, Sharareh, Hadifar, Shima, Ghajavand, Hasan, Farmanfarmaei, Ghazaleh, Fateh, Abolfazl, Siadat, Seyed Davar, Vaziri, Farzam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050728/
https://www.ncbi.nlm.nih.gov/pubmed/30016972
http://dx.doi.org/10.1186/s13104-018-3579-z
Descripción
Sumario:OBJECTIVE: Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere with the results or not. RESULTS: Based on the Spoligotyping pattern, among the fourteen patients, 57.1% had different genotypes in clinical samples and their respective cultures. These discrepant patterns were suggestive of polyclonal infections in clinical samples with possible overlapping Spoligotype patterns. We propose that in societies with high mixed infections (e.g. Iran), direct Spoligotyping on clinical samples can be controversial.

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