Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections

OBJECTIVE: Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere...

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Autores principales: Kargarpour Kamakoli, Mansour, Khanipour, Sharareh, Hadifar, Shima, Ghajavand, Hasan, Farmanfarmaei, Ghazaleh, Fateh, Abolfazl, Siadat, Seyed Davar, Vaziri, Farzam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050728/
https://www.ncbi.nlm.nih.gov/pubmed/30016972
http://dx.doi.org/10.1186/s13104-018-3579-z
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author Kargarpour Kamakoli, Mansour
Khanipour, Sharareh
Hadifar, Shima
Ghajavand, Hasan
Farmanfarmaei, Ghazaleh
Fateh, Abolfazl
Siadat, Seyed Davar
Vaziri, Farzam
author_facet Kargarpour Kamakoli, Mansour
Khanipour, Sharareh
Hadifar, Shima
Ghajavand, Hasan
Farmanfarmaei, Ghazaleh
Fateh, Abolfazl
Siadat, Seyed Davar
Vaziri, Farzam
author_sort Kargarpour Kamakoli, Mansour
collection PubMed
description OBJECTIVE: Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere with the results or not. RESULTS: Based on the Spoligotyping pattern, among the fourteen patients, 57.1% had different genotypes in clinical samples and their respective cultures. These discrepant patterns were suggestive of polyclonal infections in clinical samples with possible overlapping Spoligotype patterns. We propose that in societies with high mixed infections (e.g. Iran), direct Spoligotyping on clinical samples can be controversial.
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spelling pubmed-60507282018-07-19 Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections Kargarpour Kamakoli, Mansour Khanipour, Sharareh Hadifar, Shima Ghajavand, Hasan Farmanfarmaei, Ghazaleh Fateh, Abolfazl Siadat, Seyed Davar Vaziri, Farzam BMC Res Notes Research Note OBJECTIVE: Based on our recent studies the prevalence of polyclonal infection in tuberculosis clinical specimens is more than 50% in Tehran, Iran. With this background, Spoligotyping was performed on clinical specimens and their respective cultures, and we examined whether mixed infections interfere with the results or not. RESULTS: Based on the Spoligotyping pattern, among the fourteen patients, 57.1% had different genotypes in clinical samples and their respective cultures. These discrepant patterns were suggestive of polyclonal infections in clinical samples with possible overlapping Spoligotype patterns. We propose that in societies with high mixed infections (e.g. Iran), direct Spoligotyping on clinical samples can be controversial. BioMed Central 2018-07-17 /pmc/articles/PMC6050728/ /pubmed/30016972 http://dx.doi.org/10.1186/s13104-018-3579-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Kargarpour Kamakoli, Mansour
Khanipour, Sharareh
Hadifar, Shima
Ghajavand, Hasan
Farmanfarmaei, Ghazaleh
Fateh, Abolfazl
Siadat, Seyed Davar
Vaziri, Farzam
Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
title Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
title_full Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
title_fullStr Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
title_full_unstemmed Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
title_short Challenge in direct Spoligotyping of Mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
title_sort challenge in direct spoligotyping of mycobacterium tuberculosis: a problematic issue in the region with high prevalence of polyclonal infections
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050728/
https://www.ncbi.nlm.nih.gov/pubmed/30016972
http://dx.doi.org/10.1186/s13104-018-3579-z
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