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Cytotoxic Screening of Marine Organisms from Persian Gulf
BACKGROUND: Marine organisms produce a variety of compounds with pharmacological activities, including anticancer effects. They contain several secondary metabolites with interesting biological activities. This study attempted to find cytotoxicity of Hexane, Dichloromethane and Butanol partitions of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050974/ https://www.ncbi.nlm.nih.gov/pubmed/30069439 http://dx.doi.org/10.4103/abr.abr_9_18 |
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author | Vaseghi, Golnaz Hajakbari, Farideh Sajjadi, Shabnam Dana, Nasim Ghasemi, Ahmad Yegdaneh, Afsaneh |
author_facet | Vaseghi, Golnaz Hajakbari, Farideh Sajjadi, Shabnam Dana, Nasim Ghasemi, Ahmad Yegdaneh, Afsaneh |
author_sort | Vaseghi, Golnaz |
collection | PubMed |
description | BACKGROUND: Marine organisms produce a variety of compounds with pharmacological activities, including anticancer effects. They contain several secondary metabolites with interesting biological activities. This study attempted to find cytotoxicity of Hexane, Dichloromethane and Butanol partitions of Holothuria leucospilota and Echinometra mathaei. MATERIALS AND METHODS: H. leucospilota and E. mathaei were collected from Persian Gulf. The animals were extracted by maceration with methanol-ethyl acetate (1:1). The H. leucospilota extract was partitioned by Kupchan method to hexane, dichloromethane, butanol, and water partitions. The cytotoxic activity of the extracts was investigated against HeLa (cervical cancer) and human umbilical vein endothelial cells cell lines by mitochondrial tetrazolium test assay after 72 h. RESULTS: The cell survivals of HeLa cell were decreased by increasing the concentration of extracts. A significant reduction in cell viability at the doses of 30 (μg/ml) of dichloromethane (DCM) partition, 0.3, 3, and 30 (μg/ml) of ButOH partitions of sea cucumber, and 0.5 (μg/ml) of E. mathaei was observed. The median growth inhibitory concentration value of Hex, DCM, ButoH, and water partitions were 0.301, 0.21, 2.29, and 0.229 μg/ml, respectively. CONCLUSION: This study reveals that different partitions of H. leucospilota and total extract of E. mathaei have cytotoxic activity against cancer cell lines. More study is necessary to find the active metabolites in the more active partitions. |
format | Online Article Text |
id | pubmed-6050974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60509742018-08-01 Cytotoxic Screening of Marine Organisms from Persian Gulf Vaseghi, Golnaz Hajakbari, Farideh Sajjadi, Shabnam Dana, Nasim Ghasemi, Ahmad Yegdaneh, Afsaneh Adv Biomed Res Original Article BACKGROUND: Marine organisms produce a variety of compounds with pharmacological activities, including anticancer effects. They contain several secondary metabolites with interesting biological activities. This study attempted to find cytotoxicity of Hexane, Dichloromethane and Butanol partitions of Holothuria leucospilota and Echinometra mathaei. MATERIALS AND METHODS: H. leucospilota and E. mathaei were collected from Persian Gulf. The animals were extracted by maceration with methanol-ethyl acetate (1:1). The H. leucospilota extract was partitioned by Kupchan method to hexane, dichloromethane, butanol, and water partitions. The cytotoxic activity of the extracts was investigated against HeLa (cervical cancer) and human umbilical vein endothelial cells cell lines by mitochondrial tetrazolium test assay after 72 h. RESULTS: The cell survivals of HeLa cell were decreased by increasing the concentration of extracts. A significant reduction in cell viability at the doses of 30 (μg/ml) of dichloromethane (DCM) partition, 0.3, 3, and 30 (μg/ml) of ButOH partitions of sea cucumber, and 0.5 (μg/ml) of E. mathaei was observed. The median growth inhibitory concentration value of Hex, DCM, ButoH, and water partitions were 0.301, 0.21, 2.29, and 0.229 μg/ml, respectively. CONCLUSION: This study reveals that different partitions of H. leucospilota and total extract of E. mathaei have cytotoxic activity against cancer cell lines. More study is necessary to find the active metabolites in the more active partitions. Medknow Publications & Media Pvt Ltd 2018-07-02 /pmc/articles/PMC6050974/ /pubmed/30069439 http://dx.doi.org/10.4103/abr.abr_9_18 Text en Copyright: © 2018 Advanced Biomedical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Vaseghi, Golnaz Hajakbari, Farideh Sajjadi, Shabnam Dana, Nasim Ghasemi, Ahmad Yegdaneh, Afsaneh Cytotoxic Screening of Marine Organisms from Persian Gulf |
title | Cytotoxic Screening of Marine Organisms from Persian Gulf |
title_full | Cytotoxic Screening of Marine Organisms from Persian Gulf |
title_fullStr | Cytotoxic Screening of Marine Organisms from Persian Gulf |
title_full_unstemmed | Cytotoxic Screening of Marine Organisms from Persian Gulf |
title_short | Cytotoxic Screening of Marine Organisms from Persian Gulf |
title_sort | cytotoxic screening of marine organisms from persian gulf |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050974/ https://www.ncbi.nlm.nih.gov/pubmed/30069439 http://dx.doi.org/10.4103/abr.abr_9_18 |
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