New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells

The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing c...

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Detalles Bibliográficos
Autores principales: He, Xiangjun, Li, Yin-Xiong, Feng, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051061/
https://www.ncbi.nlm.nih.gov/pubmed/30057628
http://dx.doi.org/10.1155/2018/9465028
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author He, Xiangjun
Li, Yin-Xiong
Feng, Bo
author_facet He, Xiangjun
Li, Yin-Xiong
Feng, Bo
author_sort He, Xiangjun
collection PubMed
description The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC.
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spelling pubmed-60510612018-07-29 New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells He, Xiangjun Li, Yin-Xiong Feng, Bo Stem Cells Int Review Article The groundbreaking CRISPR technology is revolutionizing biomedical research with its superior simplicity, high efficiency, and robust accuracy. Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC. Hindawi 2018-07-03 /pmc/articles/PMC6051061/ /pubmed/30057628 http://dx.doi.org/10.1155/2018/9465028 Text en Copyright © 2018 Xiangjun He et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
He, Xiangjun
Li, Yin-Xiong
Feng, Bo
New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_full New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_fullStr New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_full_unstemmed New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_short New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells
title_sort new turns for high efficiency knock-in of large dna in human pluripotent stem cells
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051061/
https://www.ncbi.nlm.nih.gov/pubmed/30057628
http://dx.doi.org/10.1155/2018/9465028
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