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Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83

Photodynamic therapy (PDT) is a noninvasive, highly selective approach to the treatment of tumors. However, its therapeutic effect is limited by long‐lasting skin phototoxicity. Therefore, to compromise this shortcoming, it is preferable to deliver photosensitizers selectively to tumor cells with th...

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Detalles Bibliográficos
Autores principales: Ye, Ziyu, Liang, Yanfang, Ma, Yan, Lin, Bihua, Cao, Longbin, Wang, Bin, Zhang, Zhao, Yu, Haibo, Li, Jixia, Huang, Mingyuan, Zhou, Keyuan, Zhang, Qunzhou, Liu, Xinguang, Zeng, Jincheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051178/
https://www.ncbi.nlm.nih.gov/pubmed/29856138
http://dx.doi.org/10.1002/cam4.1601
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author Ye, Ziyu
Liang, Yanfang
Ma, Yan
Lin, Bihua
Cao, Longbin
Wang, Bin
Zhang, Zhao
Yu, Haibo
Li, Jixia
Huang, Mingyuan
Zhou, Keyuan
Zhang, Qunzhou
Liu, Xinguang
Zeng, Jincheng
author_facet Ye, Ziyu
Liang, Yanfang
Ma, Yan
Lin, Bihua
Cao, Longbin
Wang, Bin
Zhang, Zhao
Yu, Haibo
Li, Jixia
Huang, Mingyuan
Zhou, Keyuan
Zhang, Qunzhou
Liu, Xinguang
Zeng, Jincheng
author_sort Ye, Ziyu
collection PubMed
description Photodynamic therapy (PDT) is a noninvasive, highly selective approach to the treatment of tumors. However, its therapeutic effect is limited by long‐lasting skin phototoxicity. Therefore, to compromise this shortcoming, it is preferable to deliver photosensitizers selectively to tumor cells with the aid of antibodies specific against tumor‐associated antigens. Cancer/testis antigens 83 (CT83), also called KK‐LC‐1 or CXorf61, recognized by cytotoxic T lymphocytes (CTL), has become a promising target for immunotherapy. Herein, we developed and characterized a novel mouse CT83 mAb 7G4 with a high affinity with Gallium (III) 5, 10, 15‐tris (ethoxycarbonyl) corrole (1‐Ga), a new and promising photosensitizer in PDT. The enzyme‐linked immunosorbent assay (ELISA), flow cytometry and cytotoxicity activity assays revealed that 7G4‐1‐Ga was able to recognize human CT83 with high specificity. Furthermore, 7G4‐1‐Ga showed greater cytotoxicity to CT83‐expressing human cancer cells in vitro than 1‐Ga. These results suggest that the antibody‐conjugated photosensitizer between anti‐CT83 mAb and 1‐Ga may have a good application in PDT, where the destruction of CT83‐expressing tumor is required.
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spelling pubmed-60511782018-07-20 Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83 Ye, Ziyu Liang, Yanfang Ma, Yan Lin, Bihua Cao, Longbin Wang, Bin Zhang, Zhao Yu, Haibo Li, Jixia Huang, Mingyuan Zhou, Keyuan Zhang, Qunzhou Liu, Xinguang Zeng, Jincheng Cancer Med Clinical Cancer Research Photodynamic therapy (PDT) is a noninvasive, highly selective approach to the treatment of tumors. However, its therapeutic effect is limited by long‐lasting skin phototoxicity. Therefore, to compromise this shortcoming, it is preferable to deliver photosensitizers selectively to tumor cells with the aid of antibodies specific against tumor‐associated antigens. Cancer/testis antigens 83 (CT83), also called KK‐LC‐1 or CXorf61, recognized by cytotoxic T lymphocytes (CTL), has become a promising target for immunotherapy. Herein, we developed and characterized a novel mouse CT83 mAb 7G4 with a high affinity with Gallium (III) 5, 10, 15‐tris (ethoxycarbonyl) corrole (1‐Ga), a new and promising photosensitizer in PDT. The enzyme‐linked immunosorbent assay (ELISA), flow cytometry and cytotoxicity activity assays revealed that 7G4‐1‐Ga was able to recognize human CT83 with high specificity. Furthermore, 7G4‐1‐Ga showed greater cytotoxicity to CT83‐expressing human cancer cells in vitro than 1‐Ga. These results suggest that the antibody‐conjugated photosensitizer between anti‐CT83 mAb and 1‐Ga may have a good application in PDT, where the destruction of CT83‐expressing tumor is required. John Wiley and Sons Inc. 2018-06-01 /pmc/articles/PMC6051178/ /pubmed/29856138 http://dx.doi.org/10.1002/cam4.1601 Text en © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Cancer Research
Ye, Ziyu
Liang, Yanfang
Ma, Yan
Lin, Bihua
Cao, Longbin
Wang, Bin
Zhang, Zhao
Yu, Haibo
Li, Jixia
Huang, Mingyuan
Zhou, Keyuan
Zhang, Qunzhou
Liu, Xinguang
Zeng, Jincheng
Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83
title Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83
title_full Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83
title_fullStr Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83
title_full_unstemmed Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83
title_short Targeted photodynamic therapy of cancer using a novel gallium (III) tris (ethoxycarbonyl) corrole conjugated‐mAb directed against cancer/testis antigens 83
title_sort targeted photodynamic therapy of cancer using a novel gallium (iii) tris (ethoxycarbonyl) corrole conjugated‐mab directed against cancer/testis antigens 83
topic Clinical Cancer Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051178/
https://www.ncbi.nlm.nih.gov/pubmed/29856138
http://dx.doi.org/10.1002/cam4.1601
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