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Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study

The link of nasopharyngeal carcinoma (NPC) with Epstein‐Barr virus (EBV) has been established for decades. Although an abnormal high level of EBV sero‐antibody spectrum and cell‐free circulating EBV DNA loads were exhibited in NPC patients, oral EBV DNA loads, which are primarily responsible for the...

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Autores principales: Xue, Wen‐Qiong, He, Yong‐Qiao, Liao, Xiao‐Yu, Li, Fang‐Fang, Xu, Ya‐Fei, Xu, Feng‐Hua, Li, Xi‐Zhao, Feng, Qi‐Sheng, Chen, Li‐Zhen, Cao, Su‐Mei, Liu, Qing, Zeng, Yi‐Xin, Jia, Wei‐Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051183/
https://www.ncbi.nlm.nih.gov/pubmed/29905022
http://dx.doi.org/10.1002/cam4.1597
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author Xue, Wen‐Qiong
He, Yong‐Qiao
Liao, Xiao‐Yu
Li, Fang‐Fang
Xu, Ya‐Fei
Xu, Feng‐Hua
Li, Xi‐Zhao
Feng, Qi‐Sheng
Chen, Li‐Zhen
Cao, Su‐Mei
Liu, Qing
Zeng, Yi‐Xin
Jia, Wei‐Hua
author_facet Xue, Wen‐Qiong
He, Yong‐Qiao
Liao, Xiao‐Yu
Li, Fang‐Fang
Xu, Ya‐Fei
Xu, Feng‐Hua
Li, Xi‐Zhao
Feng, Qi‐Sheng
Chen, Li‐Zhen
Cao, Su‐Mei
Liu, Qing
Zeng, Yi‐Xin
Jia, Wei‐Hua
author_sort Xue, Wen‐Qiong
collection PubMed
description The link of nasopharyngeal carcinoma (NPC) with Epstein‐Barr virus (EBV) has been established for decades. Although an abnormal high level of EBV sero‐antibody spectrum and cell‐free circulating EBV DNA loads were exhibited in NPC patients, oral EBV DNA loads, which are primarily responsible for the EBV transmission, has not been previously studied in NPC patients. We conducted an epidemiological study to measure the oral EBV loads, viral components, and the relationship with the serum antibody titers in a large case‐control population (968 cases and 1656 controls) and a family‐based population (91 cases and 165 unaffected family members). EBV DNA loads were detected by quantitative PCR approach targeting the BamHI‐W region. Although a large individualized variation existed, we still observed a decreased oral EBV DNA loads in the population of NPC patients compared to that of healthy controls (ORs were 1.00, 0.69, 0.62, 0.33 classified by the quartiles of viral loads, P (trend) < .001) and family members. In contrast, the elevated levels of oral EBV loads were present in asymptomatic males and elders, suggesting a different important source for EBV transmission. Notably, oral EBV loads were inversely associated with serum antibody titers of VCA‐IgA, EA‐IgA (All P (trend) < .001) in the cases but not in the controls. Our study provides the first epidemiological data of oral EBV loads and viral components in NPC patients and controls in the highest risk area of Southern China, indicating that NPC status is unlikely to be an important determinant of EBV transmission.
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spelling pubmed-60511832018-07-20 Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study Xue, Wen‐Qiong He, Yong‐Qiao Liao, Xiao‐Yu Li, Fang‐Fang Xu, Ya‐Fei Xu, Feng‐Hua Li, Xi‐Zhao Feng, Qi‐Sheng Chen, Li‐Zhen Cao, Su‐Mei Liu, Qing Zeng, Yi‐Xin Jia, Wei‐Hua Cancer Med Cancer Prevention The link of nasopharyngeal carcinoma (NPC) with Epstein‐Barr virus (EBV) has been established for decades. Although an abnormal high level of EBV sero‐antibody spectrum and cell‐free circulating EBV DNA loads were exhibited in NPC patients, oral EBV DNA loads, which are primarily responsible for the EBV transmission, has not been previously studied in NPC patients. We conducted an epidemiological study to measure the oral EBV loads, viral components, and the relationship with the serum antibody titers in a large case‐control population (968 cases and 1656 controls) and a family‐based population (91 cases and 165 unaffected family members). EBV DNA loads were detected by quantitative PCR approach targeting the BamHI‐W region. Although a large individualized variation existed, we still observed a decreased oral EBV DNA loads in the population of NPC patients compared to that of healthy controls (ORs were 1.00, 0.69, 0.62, 0.33 classified by the quartiles of viral loads, P (trend) < .001) and family members. In contrast, the elevated levels of oral EBV loads were present in asymptomatic males and elders, suggesting a different important source for EBV transmission. Notably, oral EBV loads were inversely associated with serum antibody titers of VCA‐IgA, EA‐IgA (All P (trend) < .001) in the cases but not in the controls. Our study provides the first epidemiological data of oral EBV loads and viral components in NPC patients and controls in the highest risk area of Southern China, indicating that NPC status is unlikely to be an important determinant of EBV transmission. John Wiley and Sons Inc. 2018-06-14 /pmc/articles/PMC6051183/ /pubmed/29905022 http://dx.doi.org/10.1002/cam4.1597 Text en © 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cancer Prevention
Xue, Wen‐Qiong
He, Yong‐Qiao
Liao, Xiao‐Yu
Li, Fang‐Fang
Xu, Ya‐Fei
Xu, Feng‐Hua
Li, Xi‐Zhao
Feng, Qi‐Sheng
Chen, Li‐Zhen
Cao, Su‐Mei
Liu, Qing
Zeng, Yi‐Xin
Jia, Wei‐Hua
Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study
title Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study
title_full Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study
title_fullStr Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study
title_full_unstemmed Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study
title_short Decreased oral Epstein‐Barr virus DNA loads in patients with nasopharyngeal carcinoma in Southern China: A case‐control and a family‐based study
title_sort decreased oral epstein‐barr virus dna loads in patients with nasopharyngeal carcinoma in southern china: a case‐control and a family‐based study
topic Cancer Prevention
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051183/
https://www.ncbi.nlm.nih.gov/pubmed/29905022
http://dx.doi.org/10.1002/cam4.1597
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