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A novel multiplex assay of SNP-STR markers for forensic purpose

Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase...

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Autores principales: Wei, Tian, Liao, Fei, Wang, Yaowu, Pan, Chao, Xiao, Chao, Huang, Daixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051632/
https://www.ncbi.nlm.nih.gov/pubmed/30020969
http://dx.doi.org/10.1371/journal.pone.0200700
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author Wei, Tian
Liao, Fei
Wang, Yaowu
Pan, Chao
Xiao, Chao
Huang, Daixin
author_facet Wei, Tian
Liao, Fei
Wang, Yaowu
Pan, Chao
Xiao, Chao
Huang, Daixin
author_sort Wei, Tian
collection PubMed
description Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase chain reaction (PCR) assay was developed for amplifying these SNP-STRs and the Amelogenin gender marker according to the principle of amplification refractory mutation system (ARMS). This novel multiplex set allows detection of the minor DNA contributor in a DNA mixture of any gender and cellular origin with high resolution (beyond a DNA ratio of 1:20). In addition, SNP-STR haplotype frequencies were estimated based on a survey of 350 unrelated individuals from Chinese Han population, and the combined power of discrimination (PD) and power of exclusion (PE) of the eight SNP-STRs were calculated as 0.99999999965 and 0.9996, which were obviously higher than that of the eight STR loci: 0.9999999954 and 0.9989 respectively. The results indicated that the SNP-STR compound markers have higher application value in forensic identification compared to standard autosomal STRs, especially in the analysis of imbalanced DNA mixtures.
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spelling pubmed-60516322018-07-27 A novel multiplex assay of SNP-STR markers for forensic purpose Wei, Tian Liao, Fei Wang, Yaowu Pan, Chao Xiao, Chao Huang, Daixin PLoS One Research Article Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase chain reaction (PCR) assay was developed for amplifying these SNP-STRs and the Amelogenin gender marker according to the principle of amplification refractory mutation system (ARMS). This novel multiplex set allows detection of the minor DNA contributor in a DNA mixture of any gender and cellular origin with high resolution (beyond a DNA ratio of 1:20). In addition, SNP-STR haplotype frequencies were estimated based on a survey of 350 unrelated individuals from Chinese Han population, and the combined power of discrimination (PD) and power of exclusion (PE) of the eight SNP-STRs were calculated as 0.99999999965 and 0.9996, which were obviously higher than that of the eight STR loci: 0.9999999954 and 0.9989 respectively. The results indicated that the SNP-STR compound markers have higher application value in forensic identification compared to standard autosomal STRs, especially in the analysis of imbalanced DNA mixtures. Public Library of Science 2018-07-18 /pmc/articles/PMC6051632/ /pubmed/30020969 http://dx.doi.org/10.1371/journal.pone.0200700 Text en © 2018 Wei et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wei, Tian
Liao, Fei
Wang, Yaowu
Pan, Chao
Xiao, Chao
Huang, Daixin
A novel multiplex assay of SNP-STR markers for forensic purpose
title A novel multiplex assay of SNP-STR markers for forensic purpose
title_full A novel multiplex assay of SNP-STR markers for forensic purpose
title_fullStr A novel multiplex assay of SNP-STR markers for forensic purpose
title_full_unstemmed A novel multiplex assay of SNP-STR markers for forensic purpose
title_short A novel multiplex assay of SNP-STR markers for forensic purpose
title_sort novel multiplex assay of snp-str markers for forensic purpose
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051632/
https://www.ncbi.nlm.nih.gov/pubmed/30020969
http://dx.doi.org/10.1371/journal.pone.0200700
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