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A novel multiplex assay of SNP-STR markers for forensic purpose
Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051632/ https://www.ncbi.nlm.nih.gov/pubmed/30020969 http://dx.doi.org/10.1371/journal.pone.0200700 |
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author | Wei, Tian Liao, Fei Wang, Yaowu Pan, Chao Xiao, Chao Huang, Daixin |
author_facet | Wei, Tian Liao, Fei Wang, Yaowu Pan, Chao Xiao, Chao Huang, Daixin |
author_sort | Wei, Tian |
collection | PubMed |
description | Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase chain reaction (PCR) assay was developed for amplifying these SNP-STRs and the Amelogenin gender marker according to the principle of amplification refractory mutation system (ARMS). This novel multiplex set allows detection of the minor DNA contributor in a DNA mixture of any gender and cellular origin with high resolution (beyond a DNA ratio of 1:20). In addition, SNP-STR haplotype frequencies were estimated based on a survey of 350 unrelated individuals from Chinese Han population, and the combined power of discrimination (PD) and power of exclusion (PE) of the eight SNP-STRs were calculated as 0.99999999965 and 0.9996, which were obviously higher than that of the eight STR loci: 0.9999999954 and 0.9989 respectively. The results indicated that the SNP-STR compound markers have higher application value in forensic identification compared to standard autosomal STRs, especially in the analysis of imbalanced DNA mixtures. |
format | Online Article Text |
id | pubmed-6051632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60516322018-07-27 A novel multiplex assay of SNP-STR markers for forensic purpose Wei, Tian Liao, Fei Wang, Yaowu Pan, Chao Xiao, Chao Huang, Daixin PLoS One Research Article Like DIP-STR markers (deletion/insertion polymorphism-short tandem repeat combinations), SNP-STR markers (single nucleotide polymorphism-STR combinations) are also valuable in forensic DNA mixture analysis. In this study, eight SNP-STRs were selected, and a stable and sensitive multiplex polymerase chain reaction (PCR) assay was developed for amplifying these SNP-STRs and the Amelogenin gender marker according to the principle of amplification refractory mutation system (ARMS). This novel multiplex set allows detection of the minor DNA contributor in a DNA mixture of any gender and cellular origin with high resolution (beyond a DNA ratio of 1:20). In addition, SNP-STR haplotype frequencies were estimated based on a survey of 350 unrelated individuals from Chinese Han population, and the combined power of discrimination (PD) and power of exclusion (PE) of the eight SNP-STRs were calculated as 0.99999999965 and 0.9996, which were obviously higher than that of the eight STR loci: 0.9999999954 and 0.9989 respectively. The results indicated that the SNP-STR compound markers have higher application value in forensic identification compared to standard autosomal STRs, especially in the analysis of imbalanced DNA mixtures. Public Library of Science 2018-07-18 /pmc/articles/PMC6051632/ /pubmed/30020969 http://dx.doi.org/10.1371/journal.pone.0200700 Text en © 2018 Wei et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wei, Tian Liao, Fei Wang, Yaowu Pan, Chao Xiao, Chao Huang, Daixin A novel multiplex assay of SNP-STR markers for forensic purpose |
title | A novel multiplex assay of SNP-STR markers for forensic purpose |
title_full | A novel multiplex assay of SNP-STR markers for forensic purpose |
title_fullStr | A novel multiplex assay of SNP-STR markers for forensic purpose |
title_full_unstemmed | A novel multiplex assay of SNP-STR markers for forensic purpose |
title_short | A novel multiplex assay of SNP-STR markers for forensic purpose |
title_sort | novel multiplex assay of snp-str markers for forensic purpose |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051632/ https://www.ncbi.nlm.nih.gov/pubmed/30020969 http://dx.doi.org/10.1371/journal.pone.0200700 |
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