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The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II

BACKGROUND: High-NaCl diet is a contributing factor for cardiac hypertrophy. The role of HSP22 as a protective protein during cardiac hypertrophy due to hypernatremia is unclear. Accordingly, this study aimed to establish a cellular hypernatremic H9C2 model and to compare the expression of HSP22 in...

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Autores principales: Sung, Jae Hwi, Song, Ahran, Park, Taegun, Kim, Eunyoung, Lee, Seunggwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Electrolyte Metabolism 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051945/
https://www.ncbi.nlm.nih.gov/pubmed/30046328
http://dx.doi.org/10.5049/EBP.2018.16.1.1
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author Sung, Jae Hwi
Song, Ahran
Park, Taegun
Kim, Eunyoung
Lee, Seunggwan
author_facet Sung, Jae Hwi
Song, Ahran
Park, Taegun
Kim, Eunyoung
Lee, Seunggwan
author_sort Sung, Jae Hwi
collection PubMed
description BACKGROUND: High-NaCl diet is a contributing factor for cardiac hypertrophy. The role of HSP22 as a protective protein during cardiac hypertrophy due to hypernatremia is unclear. Accordingly, this study aimed to establish a cellular hypernatremic H9C2 model and to compare the expression of HSP22 in Ca(2+) homeostasis between a high-NaCl and angiotensin II-induced hypertrophic cellular H9C2 model. METHODS: Real-time PCR was performed to compare the mRNA expression. Flow cytometry and confocal microscopy were used to analyze the cells. RESULTS: The addition of 30 mM NaCl for 48 h was the most effective condition for the induction of hypertrophic H9C2 cells (termed the in vitro hypernatremic model). Cardiac cellular hypertrophy was induced with 30 mM NaCl and 1 µM angiotensin II for 48 h, without causing abnormal morphological changes or cytotoxicity of the culture conditions. HSP22 contains a similar domain to that found in the consensus sequences of the late embryogenesis abundant protein group 3 from Artemia. The expression of HSP22 gradually decreased in the in vitro hypernatremic model. In contrast to the in vitro hypernatremic model, HSP22 increased after exposure to angiotensin II for 48 h. Intracellular Ca(2+) decreased in the angiotensin II model and further decreased in the in vitro hypernatremic model. Impaired intracellular Ca(2+) homeostasis was more evident in the in vitro hypernatremic model. CONCLUSION: The results showed that NaCl significantly decreased HSP22. Decreased HSP22, due to the hypernatremic condition, affected the Ca(2+) homeostasis in the H9C2 cells. Therefore, hypernatremia induces cellular hypertrophy via impaired Ca(2+) homeostasis. The additional mechanisms of HSP22 need to be explored further.
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spelling pubmed-60519452018-07-25 The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II Sung, Jae Hwi Song, Ahran Park, Taegun Kim, Eunyoung Lee, Seunggwan Electrolyte Blood Press Clinical Article BACKGROUND: High-NaCl diet is a contributing factor for cardiac hypertrophy. The role of HSP22 as a protective protein during cardiac hypertrophy due to hypernatremia is unclear. Accordingly, this study aimed to establish a cellular hypernatremic H9C2 model and to compare the expression of HSP22 in Ca(2+) homeostasis between a high-NaCl and angiotensin II-induced hypertrophic cellular H9C2 model. METHODS: Real-time PCR was performed to compare the mRNA expression. Flow cytometry and confocal microscopy were used to analyze the cells. RESULTS: The addition of 30 mM NaCl for 48 h was the most effective condition for the induction of hypertrophic H9C2 cells (termed the in vitro hypernatremic model). Cardiac cellular hypertrophy was induced with 30 mM NaCl and 1 µM angiotensin II for 48 h, without causing abnormal morphological changes or cytotoxicity of the culture conditions. HSP22 contains a similar domain to that found in the consensus sequences of the late embryogenesis abundant protein group 3 from Artemia. The expression of HSP22 gradually decreased in the in vitro hypernatremic model. In contrast to the in vitro hypernatremic model, HSP22 increased after exposure to angiotensin II for 48 h. Intracellular Ca(2+) decreased in the angiotensin II model and further decreased in the in vitro hypernatremic model. Impaired intracellular Ca(2+) homeostasis was more evident in the in vitro hypernatremic model. CONCLUSION: The results showed that NaCl significantly decreased HSP22. Decreased HSP22, due to the hypernatremic condition, affected the Ca(2+) homeostasis in the H9C2 cells. Therefore, hypernatremia induces cellular hypertrophy via impaired Ca(2+) homeostasis. The additional mechanisms of HSP22 need to be explored further. The Korean Society of Electrolyte Metabolism 2018-06 2018-06-30 /pmc/articles/PMC6051945/ /pubmed/30046328 http://dx.doi.org/10.5049/EBP.2018.16.1.1 Text en Copyright © 2018 The Korean Society of Electrolyte Metabolism http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Article
Sung, Jae Hwi
Song, Ahran
Park, Taegun
Kim, Eunyoung
Lee, Seunggwan
The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
title The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
title_full The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
title_fullStr The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
title_full_unstemmed The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
title_short The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
title_sort different expression patterns of hsp22, a late embryogenesis abundant-like protein, in hypertrophic h9c2 cells induced by nacl and angiotensin ii
topic Clinical Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051945/
https://www.ncbi.nlm.nih.gov/pubmed/30046328
http://dx.doi.org/10.5049/EBP.2018.16.1.1
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