Cargando…
The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II
BACKGROUND: High-NaCl diet is a contributing factor for cardiac hypertrophy. The role of HSP22 as a protective protein during cardiac hypertrophy due to hypernatremia is unclear. Accordingly, this study aimed to establish a cellular hypernatremic H9C2 model and to compare the expression of HSP22 in...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Electrolyte Metabolism
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051945/ https://www.ncbi.nlm.nih.gov/pubmed/30046328 http://dx.doi.org/10.5049/EBP.2018.16.1.1 |
_version_ | 1783340579671769088 |
---|---|
author | Sung, Jae Hwi Song, Ahran Park, Taegun Kim, Eunyoung Lee, Seunggwan |
author_facet | Sung, Jae Hwi Song, Ahran Park, Taegun Kim, Eunyoung Lee, Seunggwan |
author_sort | Sung, Jae Hwi |
collection | PubMed |
description | BACKGROUND: High-NaCl diet is a contributing factor for cardiac hypertrophy. The role of HSP22 as a protective protein during cardiac hypertrophy due to hypernatremia is unclear. Accordingly, this study aimed to establish a cellular hypernatremic H9C2 model and to compare the expression of HSP22 in Ca(2+) homeostasis between a high-NaCl and angiotensin II-induced hypertrophic cellular H9C2 model. METHODS: Real-time PCR was performed to compare the mRNA expression. Flow cytometry and confocal microscopy were used to analyze the cells. RESULTS: The addition of 30 mM NaCl for 48 h was the most effective condition for the induction of hypertrophic H9C2 cells (termed the in vitro hypernatremic model). Cardiac cellular hypertrophy was induced with 30 mM NaCl and 1 µM angiotensin II for 48 h, without causing abnormal morphological changes or cytotoxicity of the culture conditions. HSP22 contains a similar domain to that found in the consensus sequences of the late embryogenesis abundant protein group 3 from Artemia. The expression of HSP22 gradually decreased in the in vitro hypernatremic model. In contrast to the in vitro hypernatremic model, HSP22 increased after exposure to angiotensin II for 48 h. Intracellular Ca(2+) decreased in the angiotensin II model and further decreased in the in vitro hypernatremic model. Impaired intracellular Ca(2+) homeostasis was more evident in the in vitro hypernatremic model. CONCLUSION: The results showed that NaCl significantly decreased HSP22. Decreased HSP22, due to the hypernatremic condition, affected the Ca(2+) homeostasis in the H9C2 cells. Therefore, hypernatremia induces cellular hypertrophy via impaired Ca(2+) homeostasis. The additional mechanisms of HSP22 need to be explored further. |
format | Online Article Text |
id | pubmed-6051945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Society of Electrolyte Metabolism |
record_format | MEDLINE/PubMed |
spelling | pubmed-60519452018-07-25 The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II Sung, Jae Hwi Song, Ahran Park, Taegun Kim, Eunyoung Lee, Seunggwan Electrolyte Blood Press Clinical Article BACKGROUND: High-NaCl diet is a contributing factor for cardiac hypertrophy. The role of HSP22 as a protective protein during cardiac hypertrophy due to hypernatremia is unclear. Accordingly, this study aimed to establish a cellular hypernatremic H9C2 model and to compare the expression of HSP22 in Ca(2+) homeostasis between a high-NaCl and angiotensin II-induced hypertrophic cellular H9C2 model. METHODS: Real-time PCR was performed to compare the mRNA expression. Flow cytometry and confocal microscopy were used to analyze the cells. RESULTS: The addition of 30 mM NaCl for 48 h was the most effective condition for the induction of hypertrophic H9C2 cells (termed the in vitro hypernatremic model). Cardiac cellular hypertrophy was induced with 30 mM NaCl and 1 µM angiotensin II for 48 h, without causing abnormal morphological changes or cytotoxicity of the culture conditions. HSP22 contains a similar domain to that found in the consensus sequences of the late embryogenesis abundant protein group 3 from Artemia. The expression of HSP22 gradually decreased in the in vitro hypernatremic model. In contrast to the in vitro hypernatremic model, HSP22 increased after exposure to angiotensin II for 48 h. Intracellular Ca(2+) decreased in the angiotensin II model and further decreased in the in vitro hypernatremic model. Impaired intracellular Ca(2+) homeostasis was more evident in the in vitro hypernatremic model. CONCLUSION: The results showed that NaCl significantly decreased HSP22. Decreased HSP22, due to the hypernatremic condition, affected the Ca(2+) homeostasis in the H9C2 cells. Therefore, hypernatremia induces cellular hypertrophy via impaired Ca(2+) homeostasis. The additional mechanisms of HSP22 need to be explored further. The Korean Society of Electrolyte Metabolism 2018-06 2018-06-30 /pmc/articles/PMC6051945/ /pubmed/30046328 http://dx.doi.org/10.5049/EBP.2018.16.1.1 Text en Copyright © 2018 The Korean Society of Electrolyte Metabolism http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Article Sung, Jae Hwi Song, Ahran Park, Taegun Kim, Eunyoung Lee, Seunggwan The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II |
title | The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II |
title_full | The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II |
title_fullStr | The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II |
title_full_unstemmed | The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II |
title_short | The Different Expression Patterns of HSP22, a Late Embryogenesis Abundant-like Protein, in Hypertrophic H9C2 Cells Induced by NaCl and Angiotensin II |
title_sort | different expression patterns of hsp22, a late embryogenesis abundant-like protein, in hypertrophic h9c2 cells induced by nacl and angiotensin ii |
topic | Clinical Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051945/ https://www.ncbi.nlm.nih.gov/pubmed/30046328 http://dx.doi.org/10.5049/EBP.2018.16.1.1 |
work_keys_str_mv | AT sungjaehwi thedifferentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT songahran thedifferentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT parktaegun thedifferentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT kimeunyoung thedifferentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT leeseunggwan thedifferentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT sungjaehwi differentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT songahran differentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT parktaegun differentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT kimeunyoung differentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii AT leeseunggwan differentexpressionpatternsofhsp22alateembryogenesisabundantlikeproteininhypertrophich9c2cellsinducedbynaclandangiotensinii |