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Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats
Spirochaetes are frequently detected in anoxic hydrocarbon- and organohalide-polluted groundwater, but their role in such ecosystems has remained unclear. To address this, we studied a sulfate-reducing, naphthalene-degrading enrichment culture, mainly comprising the sulfate reducer Desulfobacterium...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052044/ https://www.ncbi.nlm.nih.gov/pubmed/29849169 http://dx.doi.org/10.1038/s41396-018-0148-3 |
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author | Dong, Xiyang Greening, Chris Brüls, Thomas Conrad, Ralf Guo, Kun Blaskowski, Svenja Kaschani, Farnusch Kaiser, Markus Laban, Nidal Abu Meckenstock, Rainer U. |
author_facet | Dong, Xiyang Greening, Chris Brüls, Thomas Conrad, Ralf Guo, Kun Blaskowski, Svenja Kaschani, Farnusch Kaiser, Markus Laban, Nidal Abu Meckenstock, Rainer U. |
author_sort | Dong, Xiyang |
collection | PubMed |
description | Spirochaetes are frequently detected in anoxic hydrocarbon- and organohalide-polluted groundwater, but their role in such ecosystems has remained unclear. To address this, we studied a sulfate-reducing, naphthalene-degrading enrichment culture, mainly comprising the sulfate reducer Desulfobacterium N47 and the rod-shaped Spirochete Rectinema cohabitans HM. Genome sequencing and proteome analysis suggested that the Spirochete is an obligate fermenter that catabolizes proteins and carbohydrates, resulting in acetate, ethanol, and molecular hydrogen (H(2)) production. Physiological experiments inferred that hydrogen is an important link between the two bacteria in the enrichment culture, with H(2) derived from fermentation by R. cohabitans used as reductant for sulfate reduction by Desulfobacterium N47. Differential proteomics and physiological experiments showed that R. cohabitans utilizes biomass (proteins and carbohydrates) released from dead cells of Desulfobacterium N47. Further comparative and community genome analyses indicated that other Rectinema phylotypes are widespread in contaminated environments and may perform a hydrogenogenic fermentative lifestyle similar to R. cohabitans. Together, these findings indicate that environmental Spirochaetes scavenge detrital biomass and in turn drive necromass recycling at anoxic hydrocarbon-contaminated sites and potentially other habitats. |
format | Online Article Text |
id | pubmed-6052044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60520442018-07-24 Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats Dong, Xiyang Greening, Chris Brüls, Thomas Conrad, Ralf Guo, Kun Blaskowski, Svenja Kaschani, Farnusch Kaiser, Markus Laban, Nidal Abu Meckenstock, Rainer U. ISME J Article Spirochaetes are frequently detected in anoxic hydrocarbon- and organohalide-polluted groundwater, but their role in such ecosystems has remained unclear. To address this, we studied a sulfate-reducing, naphthalene-degrading enrichment culture, mainly comprising the sulfate reducer Desulfobacterium N47 and the rod-shaped Spirochete Rectinema cohabitans HM. Genome sequencing and proteome analysis suggested that the Spirochete is an obligate fermenter that catabolizes proteins and carbohydrates, resulting in acetate, ethanol, and molecular hydrogen (H(2)) production. Physiological experiments inferred that hydrogen is an important link between the two bacteria in the enrichment culture, with H(2) derived from fermentation by R. cohabitans used as reductant for sulfate reduction by Desulfobacterium N47. Differential proteomics and physiological experiments showed that R. cohabitans utilizes biomass (proteins and carbohydrates) released from dead cells of Desulfobacterium N47. Further comparative and community genome analyses indicated that other Rectinema phylotypes are widespread in contaminated environments and may perform a hydrogenogenic fermentative lifestyle similar to R. cohabitans. Together, these findings indicate that environmental Spirochaetes scavenge detrital biomass and in turn drive necromass recycling at anoxic hydrocarbon-contaminated sites and potentially other habitats. Nature Publishing Group UK 2018-05-30 2018-08 /pmc/articles/PMC6052044/ /pubmed/29849169 http://dx.doi.org/10.1038/s41396-018-0148-3 Text en © International Society for Microbial Ecology 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dong, Xiyang Greening, Chris Brüls, Thomas Conrad, Ralf Guo, Kun Blaskowski, Svenja Kaschani, Farnusch Kaiser, Markus Laban, Nidal Abu Meckenstock, Rainer U. Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
title | Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
title_full | Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
title_fullStr | Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
title_full_unstemmed | Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
title_short | Fermentative Spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
title_sort | fermentative spirochaetes mediate necromass recycling in anoxic hydrocarbon-contaminated habitats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052044/ https://www.ncbi.nlm.nih.gov/pubmed/29849169 http://dx.doi.org/10.1038/s41396-018-0148-3 |
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