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A micron-scale surface topography design reducing cell adhesion to implanted materials

The micron-scale surface topography of implanted materials represents a complementary pathway, independent of the material biochemical properties, regulating the process of biological recognition by cells which mediate the inflammatory response to foreign bodies. Here we explore a rational design of...

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Autores principales: Robotti, Francesco, Bottan, Simone, Fraschetti, Federica, Mallone, Anna, Pellegrini, Giovanni, Lindenblatt, Nicole, Starck, Christoph, Falk, Volkmar, Poulikakos, Dimos, Ferrari, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052105/
https://www.ncbi.nlm.nih.gov/pubmed/30022037
http://dx.doi.org/10.1038/s41598-018-29167-2
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author Robotti, Francesco
Bottan, Simone
Fraschetti, Federica
Mallone, Anna
Pellegrini, Giovanni
Lindenblatt, Nicole
Starck, Christoph
Falk, Volkmar
Poulikakos, Dimos
Ferrari, Aldo
author_facet Robotti, Francesco
Bottan, Simone
Fraschetti, Federica
Mallone, Anna
Pellegrini, Giovanni
Lindenblatt, Nicole
Starck, Christoph
Falk, Volkmar
Poulikakos, Dimos
Ferrari, Aldo
author_sort Robotti, Francesco
collection PubMed
description The micron-scale surface topography of implanted materials represents a complementary pathway, independent of the material biochemical properties, regulating the process of biological recognition by cells which mediate the inflammatory response to foreign bodies. Here we explore a rational design of surface modifications in micron range to optimize a topography comprised of a symmetrical array of hexagonal pits interfering with focal adhesion establishment and maturation. When implemented on silicones and hydrogels in vitro, the anti-adhesive topography significantly reduces the adhesion of macrophages and fibroblasts and their activation toward effectors of fibrosis. In addition, long-term interaction of the cells with anti-adhesive topographies markedly hampers cell proliferation, correlating the physical inhibition of adhesion and complete spreading with the natural progress of the cell cycle. This solution for reduction in cell adhesion can be directly integrated on the outer surface of silicone implants, as well as an additive protective conformal microstructured biocellulose layer for materials that cannot be directly microstructured. Moreover, the original geometry imposed during manufacturing of the microstructured biocellulose membranes are fully retained upon in vivo exposure, suggesting a long lasting performance of these topographical features after implantation.
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spelling pubmed-60521052018-07-23 A micron-scale surface topography design reducing cell adhesion to implanted materials Robotti, Francesco Bottan, Simone Fraschetti, Federica Mallone, Anna Pellegrini, Giovanni Lindenblatt, Nicole Starck, Christoph Falk, Volkmar Poulikakos, Dimos Ferrari, Aldo Sci Rep Article The micron-scale surface topography of implanted materials represents a complementary pathway, independent of the material biochemical properties, regulating the process of biological recognition by cells which mediate the inflammatory response to foreign bodies. Here we explore a rational design of surface modifications in micron range to optimize a topography comprised of a symmetrical array of hexagonal pits interfering with focal adhesion establishment and maturation. When implemented on silicones and hydrogels in vitro, the anti-adhesive topography significantly reduces the adhesion of macrophages and fibroblasts and their activation toward effectors of fibrosis. In addition, long-term interaction of the cells with anti-adhesive topographies markedly hampers cell proliferation, correlating the physical inhibition of adhesion and complete spreading with the natural progress of the cell cycle. This solution for reduction in cell adhesion can be directly integrated on the outer surface of silicone implants, as well as an additive protective conformal microstructured biocellulose layer for materials that cannot be directly microstructured. Moreover, the original geometry imposed during manufacturing of the microstructured biocellulose membranes are fully retained upon in vivo exposure, suggesting a long lasting performance of these topographical features after implantation. Nature Publishing Group UK 2018-07-18 /pmc/articles/PMC6052105/ /pubmed/30022037 http://dx.doi.org/10.1038/s41598-018-29167-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Robotti, Francesco
Bottan, Simone
Fraschetti, Federica
Mallone, Anna
Pellegrini, Giovanni
Lindenblatt, Nicole
Starck, Christoph
Falk, Volkmar
Poulikakos, Dimos
Ferrari, Aldo
A micron-scale surface topography design reducing cell adhesion to implanted materials
title A micron-scale surface topography design reducing cell adhesion to implanted materials
title_full A micron-scale surface topography design reducing cell adhesion to implanted materials
title_fullStr A micron-scale surface topography design reducing cell adhesion to implanted materials
title_full_unstemmed A micron-scale surface topography design reducing cell adhesion to implanted materials
title_short A micron-scale surface topography design reducing cell adhesion to implanted materials
title_sort micron-scale surface topography design reducing cell adhesion to implanted materials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052105/
https://www.ncbi.nlm.nih.gov/pubmed/30022037
http://dx.doi.org/10.1038/s41598-018-29167-2
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